Influence of Radiofrequency Emf on the Yeast Sacharomyces Cerevisiae as Model Eukaryotic System

Gromozova, E. N.; Voychuk, S.I.

doi:10.1007/978-0-387-28417-0_13

Cited by 5 publications

(8 citation statements)

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“…However, analysis of experimental data has shown alteration of the statistical distribution type in September-October, 2005. The probable impact of volunteer's age and gender was discussed previously [8] and was rejected in the course of multifactorial statistic analysis. We have fi xed a linear trend in the amplitude parameters of the volunteers over 11 years, which (in accordance with the mathematical statistical rules) is inessential for the discussed distribution of statistical moments.…”

Section: Resultsmentioning

confidence: 99%

“…On the one hand, this period allows accumulation of the volume of data suffi cient for statistical analysis (at least 270 measurements), on the other hand, seasonal and annual trends of the measured parameters are taken into consideration. We deliberately do not analyze the relationships between the studied parameter and this or that human organ or system (these problem have been discussed previously [8]), as the data presented in this paper are essential for not only medical, but also biological, population, and ecological levels of the biosphere adaptation to cosmogeophysical factors.…”

Section: Methodsmentioning

confidence: 99%

“…Three-color staining (MTC-1, MTC-2, MTC-3) was characteristic of the selected cell structures, the color changes depending on variations in the space weather parameters. A detailed description of the experiment technology and equipment is presented in previous reports [1,[5][6][7][8][9] and in sites of the helio-ecological sector of Institute of Geomagnetism and Radiowave Propagation IZMI-RAN (http://helioecology.webnode.com).…”

Section: Methodsmentioning

confidence: 99%

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Effects of Space Weather on Biomedical Parameters during the Solar Activity Cycles 23-24

et al. 2015

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The results of long-term (1998-2012) biomedical monitoring of the biotropic effects of space weather are discussed. A drastic change in statistical distribution parameters in the middle of 2005 was revealed that did not conform to usual sinusoidal distribution of the biomedical data reflecting changes in the number of solar spots over a solar activity cycle. The dynamics of space weather of 2001-2012 is analyzed. The authors hypothesize that the actual change in statistical distributions corresponds to the adaptation reaction of the biosphere to nonstandard geophysical characteristics of the 24th solar activity cycle and the probable long-term decrease in solar activity up to 2067.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effects of Space Weather on Biomedical Parameters during the Solar Activity Cycles 23-24

et al. 2015

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“…Investigators have demonstrated that CuO-NPs are toxic to Escherichia coli HB101 (3, 4), prokaryotic alga Microcystis aeruginosa (5), airway epithelial (HEp-2) cells (6), the crustacean Thamnocephalus platyurus (7), Lemna gibba (8), mice (9), and other organisms. A considerable amount of research has been done about the toxicity of CuO-NPs, and comparative interspecies sensitivity was found to vary greatly; however, the mechanism of CuO-NP toxicity at the cellular level is still unclear (10-12).The yeast Saccharomyces cerevisiae as a unicellular eukaryotic model organism in studying toxicity of chemicals is typical and widely used because its structure and functional organization are similar to those of higher organisms and because the genetic background of yeast is clear (13,14). S. cerevisiae is increasingly used in the toxicity assessment of chemicals such as heavy metals (15,16) and .…”

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confidence: 99%

“…The yeast Saccharomyces cerevisiae as a unicellular eukaryotic model organism in studying toxicity of chemicals is typical and widely used because its structure and functional organization are similar to those of higher organisms and because the genetic background of yeast is clear (13,14). S. cerevisiae is increasingly used in the toxicity assessment of chemicals such as heavy metals (15,16) and .…”

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confidence: 99%

Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted

Bao

Fang

et al. 2015

Appl Environ Microbiol

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bTo develop applicable and susceptible models to evaluate the toxicity of nanoparticles, the antimicrobial effects of CuO nanoparticles (CuO-NPs) on various Saccharomyces cerevisiae (S. cerevisiae) strains (wild type, single-gene-deleted mutants, and multiple-gene-deleted mutants) were determined and compared. Further experiments were also conducted to analyze the mechanisms associated with toxicity using copper salt, bulk CuO (bCuO), carbon-shelled copper nanoparticles (C/Cu-NPs), and carbon nanoparticles (C-NPs) for comparisons. The results indicated that the growth inhibition rates of CuO-NPs for the wild-type and the single-gene-deleted strains were comparable, while for the multiple-gene deletion mutant, significantly higher toxicity was observed (P < 0.05). When the toxicity of the CuO-NPs to yeast cells was compared with the toxicities of copper salt and bCuO, we concluded that the toxicity of CuO-NPs should be attributed to soluble copper rather than to the nanoparticles. The striking difference in adverse effects of C-NPs and C/Cu-NPs with equivalent surface areas also proved this. A toxicity assay revealed that the multiple-gene-deleted mutant was significantly more sensitive to CuO-NPs than the wild type. Specifically, compared with the wild-type strain, copper was readily taken up by mutant strains when cell permeability genes were knocked out, and the mutants with deletions of genes regulated under oxidative stress (OS) were likely producing more reactive oxygen species (ROS). Hence, as mechanism-based gene inactivation could increase the susceptibility of yeast, the multiple-gene-deleted mutants should be improved model organisms to investigate the toxicity of nanoparticles. With recent developments in nanotechnology around the world, there are mounting concerns regarding the potential environmental and human health risks caused by exposure to engineered nanomaterials (ENMs). Relative to the rapid development of nanotechnology, ecotoxicology and environmental hazard assessments have obviously fallen behind and still represent huge knowledge gaps (1). It is thus urgent to establish approaches to speed up safety analysis, and the insights gained from these approaches could give us a better understanding of the hazardous effects ENMs at the biological level and assist the development of safe-design approaches.As one of the increasingly used metal oxide nanoparticles (NPs), CuO-NPs have been applied to superconducting materials, sensing materials, glass, and ceramics and in other primarily antimicrobial applications (2). The extensive production and usage of CuO-NPs increase the harmful effects to organisms. Investigators have demonstrated that CuO-NPs are toxic to Escherichia coli HB101 (3, 4), prokaryotic alga Microcystis aeruginosa (5), airway epithelial (HEp-2) cells (6), the crustacean Thamnocephalus platyurus (7), Lemna gibba (8), mice (9), and other organisms. A considerable amount of research has been done about the toxicity of CuO-NPs, and comparative interspecies sensitivity was found to vary...

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Toxicological effects of graphene oxide on Saccharomyces cerevisiae

et al. 2017

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Using as an experimental model, the potential toxicity of graphene oxide (GO) was evaluated following exposure to 0-600 mg L for 24 h. The results showed that cell proliferation was observably inhibited and the IC value was 352.704 mg L. Mortality showed a concentration-dependent increase, and was 19.3% at 600 mg L. A small number of cells were deformed and shrunken after exposure. The percentage of late apoptosis/necrosis showed a significant increase ( 0.01) at 600 mg L (19.16%) compared with the control (1.14%). The mitochondrial transmembrane potential was significantly decreased ( 0.01) at 50-600 mg L, indicating that the apoptosis was related to mitochondrial impairment. Moreover, ROS was observably increased ( 0.01) at 200, 400 and 600 mg L. The expressions of apoptosis-related genes (SOD, Yca1, Nma111 and Nuc1) were significantly changed. The results presented so far indicate that GO has the potential to cause adverse effects on organisms when released into the environment.

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Influence of Radiofrequency Emf on the Yeast Sacharomyces Cerevisiae as Model Eukaryotic System

Cited by 5 publications

References 0 publications

Effects of Space Weather on Biomedical Parameters during the Solar Activity Cycles 23-24

Effects of Space Weather on Biomedical Parameters during the Solar Activity Cycles 23-24

Assessment of the Toxicity of CuO Nanoparticles by Using Saccharomyces cerevisiae Mutants with Multiple Genes Deleted

Toxicological effects of graphene oxide on Saccharomyces cerevisiae

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