Influence of race, sex, and blood pressure on erythrocyte sodium transport in humans.

Smith, Jean B.; Wade, Mary B.; Fineberg, Naomi S.; Weinberger, Myron H.

doi:10.1161/01.hyp.12.3.251

Cited by 33 publications

(10 citation statements)

References 43 publications

(28 reference statements)

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“…When we restricted our analysis to Caucasian subjects, similar relationships were seen between AER and blood pressure (but not SLC) as in the whole group. The observation that Afro-Caribbean subjects had lower SLC than Caucasian subjects, confirms a finding previous, ly noted by other groups [21,22].…”

Section: Discussionsupporting

confidence: 89%

The relationship of urinary albumin excretion rate to ambulatory blood pressure and erythrocyte sodium-lithium countertransport in NIDDM

et al. 1995

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SummaryIncreased erythrocyte sodium-lithium countertransport rate is found in non-diabetic subjects with essential hypertension, and in insulin-dependent diabetic subjects with nephropathy. However, relationships between these variables in non-insulin-dependent diabetic subjects are ill-defined. In order to characterise the relationships between blood pressure, urinary albumin excretion, and erythrocyte sodium-lithium countertransport, 66subjects with non-insulin-dependent diabetes were studied. Urinary albumin excretion rate correlated with mean 24-h ambulatory systolic blood pressure (r = 0.57; p < 0.001), but not with sodium-lithium countertransport (r = 0.06; p = 0.31). No significant relationship was observed between 24-h systolic blood pressure and erythrocyte sodium-lithium countertransport (r= 0.16; p = 0.17). The principal differences between microalbuminuric and normoalbuminuric subjects In conclusion, albumin excretion rates in non-insulin-dependent diabetic subjects are linked to hypertension and glycaemic exposure, but show no relationship to erythrocyte sodium-lithium countertransport. [Diabetologia (1995) 38: 356-362]

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Section: Discussionsupporting

confidence: 89%

The relationship of urinary albumin excretion rate to ambulatory blood pressure and erythrocyte sodium-lithium countertransport in NIDDM

et al. 1995

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“…Because RBC Na + content is directly related to passive membrane cation permeability and inversely related to activity of the Na + -K + pump, 23 it is possible that the fall in RBC Na + content we observed is the result of an alteration in either or both parameters. Previous in vitro studies clearly demonstrate that RBC membrane cholesterol enrichment decreases both passive cation leak permeability 22 and Na + , K + -ATPase activity, 24 and membrane cholesterol depletion stimulates Na + -K + pump activity.…”

Section: Discussionmentioning

confidence: 93%

Effects of lovastatin treatment on red blood cell and platelet cation transport.

et al. 1991

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Hypercholesterolemia frequently accompanies hypertension, and it has been suggested that by affecting membrane lipid composition, hypercholesterolemia may cause or accentuate abnormalities in several red blood cell transports associated with hypertension. Such an effect might obfuscate the relation of membrane markers to hypertension and decrease their usefulness in genetic studies of the heritable basis of hypertension. To determine if changing plasma lipids affects membrane transport, we studied the effects of the cholesterol-lowering agent lovastatin on red blood cell lithium-sodium countertransport and sodium-potassium-chloride cotransport, red blood cell sodium and water content, and platelet amiloride-sensitive volume responsiveness to cytoplasmic acidification, an indirect measure of sodium-proton exchange that has been proposed as a new membrane marker for hypertension. In a 24 -week, placebo-controlled, double-blinded, randomized trial, lovastatin significantly lowered total and low density lipoprotein cholesterol and raised high density lipoprotein cholesterol. Red blood cell lithium-sodium countertransport and sodium-potassium-chloride cotransport were not significantly altered. Red blood cell sodium content decreased significantly in the lovastatintreated group, probably as a result of an increase in red blood cell sodium-potassium pump activity. Platelet amiloride-sensitive responses to cytoplasmic acidification were significantly depressed by lovastatin treatment, suggesting that lowering plasma cholesterol may suppress platelet sodium-proton exchange. It has been hypothesized that the hypeiiipidemias frequently observed in essential hypertensive patients may alter membrane lipid composition and affect membrane cation transport activities. Our observations on the effects of lovastatin treatment suggest that the abnormalities in lithium-sodium countertransport and sodium-potassiumchloride cotransport associated with human essential hypertension are unlikely to result from altered membrane cholesterol content or membrane cholesterol/phospholipid ratio. Altered membrane lipid composition may affect the sodium-potassium pump or sodium-proton antiport. olism cause or at least contribute to the increased Vnu,, for RBC Li + -Na + countertransport in hypertension.8 However, it is equally plausible that high total or low HDL cholesterol and hypertension are both features of a common underlying defect, such as that which may cause the syndrome characterized by Williams et al 9 as familial dyslipidemic hypertension. If an increased V,^ for RBC Li + -Na + countertransport is a marker for a genetic predisposition to familial dyslipidemic hypertension or related hypercholesterolemia syndromes, 10 then increased RBC Li + -Na + countertransport, hyperlipidemia, and hypertension could all be interrelated, but lipid levels per se would not necessarily contribute to elevated RBC Li + -Na + countertransport activity.We measured RBC Li + -Na + countertransport activity and plasma lipid levels in patients before and aft...

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“…Furthermore, black women have the highest prevalence of hypertension. 26 In a recent report, Smith et al 27 reported that the black female hypertensive population was distinctive in their values for several of the Na + ,K + -ATPase pump parameters. This group had higher sodium pump sites per erythrocytes compared with other black groups.…”

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confidence: 99%

“…This subgroup also displayed the highest salt sensitivity of blood pressure compared with all other hypertensive groups. 28 Smith et al 27 did not measure the affinity constant for ouabain binding to erythrocytes. Hypertension is a multifactorial disease, and we do not know if our finding of decreased ouabain affinity of the enzyme is one of the risk factors that predispose these children to essential hypertension in adulthood, but our biochemical data are in excellent agreement with the epidemiological data.…”

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confidence: 99%

Race, sex, and family history of hypertension and erythrocyte sodium pump [3H]ouabain binding.

1990

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We studied the binding properties of [ 3 H]ouabain to erythrocytes from normotensive children (n=83) between the ages of 10 and 18 years (mean resting arterial pressure: 102/57 mm Hg) from normotensive and essential hypertensive parents. Arterial blood pressures of 101/57 and 104/57 mm Hg (subjects with normotensive and hypertensive parents, respectively) were not significantly different between the two groups. Forty-four children had normotensive parents and 39 had hypertensive parents, 51 were white and 32 were black, and 41 were girls and 42 were boys. By using the [ 3 H]ouabain-binding technique, we determined the density of sodium pump sites and the equilibrium dissociation constants in erythrocytes from these children. Possible effects of race, sex, or parental hypertension status on pump sites and dissociation constants were tested with a three-way analysis of variance (ANOVA). Race had a major effect on the dissociation constant: blacks had a significantly higher value than did whites (p=0.002). We also found a race by sex by parental hypertension status interaction (p=0.04) with black girls with hypertensive parents having the highest value. There was no effect of race, sex, or status on sodium pump site density. Age, height, weight, resting arterial blood pressure, and plasma Na + and K + concentrations did not correlate with the dissociation constants. These data suggest that, among the groups we studied, black girls with hypertensive parents had erythrocytes with the lowest binding affinity for ouabain. In addition, race had a strong effect on the binding affinity of ouabain to human erythrocytes, with blacks having lower affinities than whites. Because the black population has a much higher prevalence of hypertension than the white population and black women have the highest prevalence of hypertension, this decrease in the affinity of ouabain-binding to the erythrocyte sodium pump molecule may be a possible predictor of adult hypertension. (Hypertension 1990;15:146- Parts of this study were presented at the 60th Scientific Sessions of the American Heart Association in Anaheim, California, November 16-19, 1987. Supported by research grants HL-35788 and HL-32270 from the National Institutes of Health and a Grant-in-Aid from the American Heart Association, Tennessee Affiliate.Address for correspondence: Dr. Emel Songu-Mize, Department of Pharmacology, University of Tennessee, Memphis, 874 Union Ave., Rm 100, Memphis, TN 38163.Received September 20,1988; accepted in revised form October 13, 1989. transport pathways in other tissues, such as the vasculature, have also been noted in some animal models of hypertension. 13- 16 In some reports, normotensive offspring of hypertensive individuals manifested some of these sodium transport abnormalities. -1718 In spite of extensive knowledge related to cation transport in hypertensive persons, findings related to ouabain-sensitive or the sodium pumpdriven transport system are less definitive than findings for other transport systems. Moreover, binding prop...

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Influence of race, sex, and blood pressure on erythrocyte sodium transport in humans.

Cited by 33 publications

References 43 publications

The relationship of urinary albumin excretion rate to ambulatory blood pressure and erythrocyte sodium-lithium countertransport in NIDDM

The relationship of urinary albumin excretion rate to ambulatory blood pressure and erythrocyte sodium-lithium countertransport in NIDDM

Effects of lovastatin treatment on red blood cell and platelet cation transport.

Race, sex, and family history of hypertension and erythrocyte sodium pump [3H]ouabain binding.

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