Influence of Quercetin on Biochemical and Morphological Changes in Rat Testes After 30 Days Long Central Deprivation of Luteinizing Hormone

Stetsuk, Ye. V.; Шепітько, Володимир Іванович; Akimov, O. Ye.; Yakushko, O. S.; Solovyova, N. V.

doi:10.26724/2079-8334-2021-3-77-243-248

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…W hen we studied the macrophage system of the interstitium of the testes of the experimental group, as in our previous studies, we clearly traced two populations of cells by the type of localization in the connective tissue [17]. Parietal macrophages were detected near the basement membrane of the convoluted tubule, their number increased in comparison with both the control group and the previous study periods.…”

Section: Resultssupporting

confidence: 58%

Influence of quercetin on morphological changes in rats testes after 180 days during central deprivation of luteinizing hormone

Stetsuk

Шепітько

2021

Rep. of Morph.

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A relevant and popular area of research is the protective effect of the bioflavonoid quercetin, which makes it possible to use it to correct testicular dysfunction of various origins. The aim of the study was to determine the effect of quercetin on the microscopic organization of rat testicles, nitric oxide production and the intensity of oxidative stress in rat testicles on the 180th day of the experiment, during experimental central deprivation of luteinizing hormone synthesis caused by triptorelin solution. The experiment was performed on 20 adult male white rats. Rats were divided into 2 groups of 10 animals in each group: control group (I), group with central deprivation of LH synthesis + quercetin (II). Animals from the group with central deprivation of LH synthesis were injected subcutaneously with triptorelin acetate at a dose of 0.3 mg of active substance per kg and quercetin 100 mg per kg of body weight 3 times a week, while the control group was injected with saline. Ultrathin sections made by ultramicrotome were contrasted with 1 % aqueous uranyl acetate and lead citrate according to the Reynolds method and examined by electron microscopy. According to standard methods, the material was poured into paraffin blocks, from which sections with a thickness of 4 μm were made and stained with hematoxylin and eosin. Histological specimens were examined using a Вiorex 3 light microscope with a digital microfilter with software adapted for these studies. All biochemical studies were performed in 10 % of testicular tissue homogenate using a Ulab 101 spectrophotometer. Statistical processing of the study results was performed using Microsoft Office Excel software and Real Statistics 2019 extension. The nonparametric Mann-Whitney test was used to determine the statistical significance of differences between groups. Our study of the interstitial space in the testes of white rats showed the heterogeneity of populations of endocrinocytes and macrophages and the variability of structural and functional parameters. In the tissues of the testes in conditions of prolonged central deprivation of testosterone synthesis develops oxidative stress, which on the 180th day of the experiment leads to the development of edema of the interstitial space, followed by tissue fibrosis. Changes in the polarization of macrophages in our opinion may cause oxidative stress in the testes, as evidenced by increased iNOS activity and decreased arginase activity, but use of quercetin protects rat testicular tissue from oxidative damage caused by triptorelin by increasing direct antioxidant system and effects on the lipoxygenase pathway of arachidonic acid metabolism.

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Section: Resultssupporting

confidence: 58%

Influence of quercetin on morphological changes in rats testes after 180 days during central deprivation of luteinizing hormone

Stetsuk

Шепітько

2021

Rep. of Morph.

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“…Rats were divided into 2 groups with 10 animals in each group: the control group (I), the group with central deprivation of LH synthesis + quercetin (II). Animals from the group with central deprivation of testosterone synthesis were injected subcutaneously with triptorelin acetate at a dose of 0.3 mg of the active substance per kg [5,19] and quercetin 100 mg per kg body weight 3 times a week [20], while the control group was administered saline. Experiment conducted for 90 days.…”

Section: Methodsmentioning

confidence: 99%

The effect of quercetin on the morphogenesis of the interstitial space in the testes of rats after 90 days with central blockade of luteinizing hormone

et al. 2023

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Leydig cells are responsible for the production of testosterone in the male testicles when stimulated by luteinizing hormone (LH). Dysfunction of Leydig cells, which occurs during inflammation or oxidative stress, is one of the main causes of male infertility. The purpose of the study is to determine the effect of quercetin on the microscopic organization of rat testes, nitric oxide production and the intensity of oxidative stress in rat testes on the 90th day of the experiment, during the experimental central deprivation of LH synthesis caused by the administration of triptorelin acetate solution. The experiment was conducted on 20 sexually mature male white rats. Rats were divided into 2 groups of 10 animals in each group: control group (I), group with central deprivation of LH + quercetin synthesis (II). Animals from the group with central blockade of LH synthesis were injected subcutaneously with triptorelin acetate at a dose of 0.3 mg of the active substance per kg and quercetin at 100 mg per kg of body weight 3 times a week, while the control group was injected with saline. Our study of the interstitial space in the testes of white rats showed heterogeneity of macrophage populations and variability of structural and functional parameters. Central blockade of LH synthesis by the administration of treptorelin with the parallel administration of quercitin to the studied animals on the 90th day of the experiment causes changes in the structure of the interstitial space of rat testes, which is characterized by high variability both in the populations of interstitial endocrinocytes and macrophages. Biochemical indicators on the 90th day of the experiment indicate an increase in NO production in conditions of central blocking of the synthesis of luteinizing hormone by more than three times, which is ensured by the activity of the inducible isoform of NOS (iNOS). At the same time, the increase in the activity of iNOS with a decrease in the activity of the arginase pathway leads to the polarization of macrophages according to the pro-inflammatory type. The introduction of quercetin protects the testicular tissue of rats from oxidative damage caused by the administration of triptorelin on the 90th day of the experiment by increasing antioxidant protection and reducing reactive oxygen species in the tissue.

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Influence of Quercetin on Biochemical and Morphological Changes in Rat Testes After 30 Days Long Central Deprivation of Luteinizing Hormone

Cited by 2 publications

References 13 publications

Influence of quercetin on morphological changes in rats testes after 180 days during central deprivation of luteinizing hormone

Influence of quercetin on morphological changes in rats testes after 180 days during central deprivation of luteinizing hormone

The effect of quercetin on the morphogenesis of the interstitial space in the testes of rats after 90 days with central blockade of luteinizing hormone

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