Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ

Abstract

The present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions. After 1 min of pellicle formation on bovine enamel slabs, 12 subjects rinsed with 8 ml of the fluoride test solutions (NaF, Na2PO3F, AmF, SnF2,) with 500 ppm fluoride concentration each for 1 min. Additionally, rinsing without a solution (control) and rinsing with 1563 ppm SnCl2 solution took place for 1 min. Afterwards, fluorescence microscopy took place to visualize bacterial adhesion and glucan formation (8 h oral exposition) with DAPI and ConA and the BacLight method. TEM was performed to visualize the pellicle ultrastructure together with EDX to detect stannous ions. The rinsing solutions with pure SnF2 and SnCl2 reduced significantly the initial bacterial colonization (DAPI). While, NaF and Na2PO3F showed no significant effect compared to the control. There was no significant difference between AmF, SnF2 and SnCl2. All tested experimental solutions showed no reducing effect on the glucan formation. Considerable alterations of the pellicle ultrastructure resulted from rinsing with the Sn-containing solutions. SnF2 appears to be the most effective type of fluoride to reduce initial bacterial colonization in situ. The observed effects primarily have to be attributed to the stannous ions’ content.

Impact of a toothpaste with microcrystalline hydroxyapatite on the occurrence of early childhood caries: a 1-year randomized clinical trial

“…Amine fluoride shows (in contrast to sodium fluoride or sodium monofluorophosphate) antibacterial properties due to the ammonium salt (i.e. the counterion of the fluoride) 52 , 54 . Taken together, this proves that the use of the control toothpastes with 500 ppm fluoride provided as amine fluoride has caries preventing effects on the primary dentition.…”

“…• Age 3-7 years • Complete set of fully erupted primary molars (teeth 55,54,64,65,75,74,84,85) • Presence of a restoration (filling) due to caries on at least 1 primary molar • Minimum of 4 primary molars without a restoration or sealed fissure/pit Exclusion criteria.…”

“…Due to the antibacterial fluoride counter ion (i.e. the ammonium salt), amine fluoride shows, in contrast to sodium fluoride and sodium monofluorophosphate, additional antibacterial/antibiofilm properties [52][53][54] . Additionally, a recent in situ study showed an effective remineralization of early caries lesions by a HAP toothpaste.…”

Recent Development of Active Ingredients in Mouthwashes and Toothpastes for Periodontal Diseases

“…The increase of pellicle thickness reduced the bacterial adhesion (antiadhesion effect) and the bacterial viability. Thus, the protective properties of the pellicle are shown to be improved by both stannous-containing solutions—SnF 2 and SnCl 2 [ 133 ].…”

Verwendung von Fluoriden zur Kariesprävention

“…Zudem kann Fluorid mit der Anheftung von Mikroorganismen an der Zahnoberfläche interferieren [ 16 , 17 ]. Insbesondere Zinn- und Aminfluoride zeigen diese Wirkung [ 18 ]. Dadurch kann die Entstehung einer kariesfördernden Plaque reduziert werden.…”