Influence of pub gene Expression on Differentiation of Mouse Embryonic Stem Cells into Derivatives of Ecto-, Meso-, and Endoderm in vitro

Новосадова, Е. В.; Manuilova, Ekaterina; Arsenieva, E. L.; Лебедев, А.Н.; Khaidarova, N. V.; Tarantul, V. Z.; Гривенников, И. А.

doi:10.32607/20758251-2009-1-2-93-97

Cited by 5 publications

(1 citation statement)

References 12 publications

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“…Overexpression of TRIM14 (T-cell receptor interacting molecule 14) leads to increased levels of HSP90AB1 (Nenasheva et al 2014). Trim14 increases mesodermal differentiation and inhibits ectodermal differentiation of mouse ES cells (Novosadova et al 2009). …”

Section: Inductors Of Hsp90(ab1)mentioning

confidence: 99%

HSP90AB1: Helping the good and the bad

Haase

Fitze

2016

Gene

111

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HSP90AB1 (heat shock protein 90 kDA alpha, class B, member 1), also known as HSP90beta, is a member of the large family of HSPs which function as molecular chaperones. Chaperones, by binding to client proteins, support proper protein folding and maintain protein stability, especially after exposure to various kinds of cellular stress. Client proteins belong to various protein families including kinases, ubiquitin ligases and transcription factors. HSP90 proteins act as dimers and bind clients with the help of co-chaperones. The cochaperones influence many functions including client binding, ATPase activity or ATP binding of HSP90. HSPs are necessary for a large scale of cellular processes and therefore essential for cell survival. Since client proteins can be mutant proteins that would be degraded without the help of chaperones, HSPs also promote tumor formation and cancer cell proliferation. As such, they are also targets for new therapeutic approaches in cancer treatment. This review focuses on recent studies on HSP90AB1, if possible in comparison with its close homologue HSP90AA1.

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Section: Inductors Of Hsp90(ab1)mentioning

confidence: 99%

HSP90AB1: Helping the good and the bad

Haase

Fitze

2016

Gene

111

View full text Add to dashboard Cite

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Trim14 overexpression causes the same transcriptional changes in mouse embryonic stem cells and human HEK293 cells

Nenasheva

Ковалева

Khaidarova

et al. 2013

In Vitro Cell.Dev.Biol.-Animal

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The trim14 (pub, KIAA0129) gene encodes the TRIM14 protein which is a member of the tripartite motif (TRIM) family. Previously, we revealed high expression levels of trim14 in HIV- or SIV-associated lymphomas and demonstrated the influence of trim14 on mesodermal differentiation of mouse embryonic stem cells (mESC). In the present work, to elucidate the role of trim14 in normal and pathological processes in the cell, we used two different types of cells transfected with trim14: mESC and human HEK293. Using subtractive hybridization and real-time PCR, we found a number of genes which expression was elevated in trim14-transfected mESC: hsp90ab1, prr13, pu.1, tnfrsf13c (baff-r), tnfrsf13b (taci), hlx1, hbp1, junb, and pdgfrb. A further analysis of the trim14-transfected mESC at the initial stage of differentiation (embryoid bodies (EB) formation) showed essential changes in the expression of these upregulated genes. The transfection of trim14 into HEK293 also induced an enhanced expression of the several genes upregulated in trim14-transfected mESC (hsp90ab1, prr13, pu.1, tnfrsf13c (baff-r), tnfrsf13b (taci), and hlx1). Summarizing, we found similar genes that participated in trim14-directed processes both in mESC and in HEK293. These results demonstrate the presence of the similar mechanism of trim14 gene action in different types of mammalian cells.

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The Transcriptional Changes of trim Genes Associated with Parkinson’s Disease on a Model of Human Induced Pluripotent Stem Cells

et al. 2016

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Over the last few years, in vitro models, based on patient-derived induced pluripotent stem cells (iPSCs), have received considerable attention for modeling different neurodegenerative disorders. Using this model, we analyzed transcription of 15 tripartite motif (trim) genes in iPSCs, derived from the different groups: Parkinson's disease (PD) patients bearing mutations in different genes, patient with the sporadic form of PD, and the healthy individuals. The transcription was observed during neuronal differentiation of the cells in vitro into neuronal stem cells and terminally differentiated neurons. The transcription of over 50 % of these genes, belonging to different sub-groups of the TRIM family, varied between PD patients and healthy individuals during the reprogramming of fibroblasts into iPSCs and the following neuronal differentiation. Moreover, the transcription of the trim6 and trim24 genes was different between cells, derived from PD patients, and control cells at all stages. The transcription of the four trim genes (trim5α, 26, 27, 31) remained unchanged during almost all investigated stages, compared with the controls. We suppose that the revealed changes in the transcription of several trim genes reflect their possible role in neurodegenerative processes at the early stages of PD. These genes may act as a gear unit between the PD progression and the deregulation of the immune system.

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Influence of pub gene Expression on Differentiation of Mouse Embryonic Stem Cells into Derivatives of Ecto-, Meso-, and Endoderm in vitro

Cited by 5 publications

References 12 publications

HSP90AB1: Helping the good and the bad

HSP90AB1: Helping the good and the bad

Trim14 overexpression causes the same transcriptional changes in mouse embryonic stem cells and human HEK293 cells

The Transcriptional Changes of trim Genes Associated with Parkinson’s Disease on a Model of Human Induced Pluripotent Stem Cells

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