Influence of protein supplements on the secretion of leukaemia inhibitory factor by mitomycin-pretreated Vero cells: possible application to the in vitro production of bovine blastocysts with high cryotolerance

Carnegie, Jacqueline A.; Morgan, J.; McDiarmid, N.; Durnford, R.

doi:10.1530/jrf.0.1170041

Cited by 17 publications

(10 citation statements)

References 3 publications

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“…In the present experiment, there was no significant increase in cleavage and morula production rate when the presumptive zygotes were cultured in LIF media, but a significantly improved blastocyst production and hatching rate was observed when compared with the control group. Similar results were reported by Carnegie et al (1999) while using high LIF secreting coculture system for bovine embryo production, which Figure Y Phylogenetic relationship of the leukaemia inhibitory factor (LIF) nucleotide sequences from different species using DNASTAR Version 4.0, Inc., USA following the alignment of the partial ORF sequences using ClustalW method (nucleotide p distance). resulted in improvement of blastocyst formation and also in a better pregnancy rate after transfer of cryopreserved embryos into recipient cows.…”

Section: Discussionsupporting

confidence: 77%

Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRβ) in buffalo preimplantation embryos produced in vitro: markers of successful embryo implantation

Eswari

Kumar

Sharma

2012

Zygote

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Summary The objective of this study was to evaluate the effect of supplementation of recombinant leukaemia inhibitory factor (LIF) in culture media on blastocyst development, total cell number and blastocyst hatching rates and the reverse transcription-polymerase chain reaction analysis of preimplantation buffalo embryos to determine whether they contain the LIF-encoding mRNA and its beta receptor (LIFRβ) genes in different stages of preimplantation buffalo embryos. Cumulus-oocyte complexes retrieved from slaughterhouse buffalo ovaries were matured in vitro and fertilized using frozen buffalo semen. After 18 h of co-incubation with sperm, the presumptive zygotes were cultured in modified synthetic oviductal fluid without (control) or with rhLIF (100 ng/ml). There was no significant difference in the overall cleavage rate up to morula stage however the development of blastocysts, hatching rate and total cell numbers were significantly higher in the LIF-treated group than control. Transcripts for LIFRβ were detected from immature, in vitro-matured oocytes and in the embryos up to blastocyst stage, while transcripts for the LIF were detected from 8-16-cell stage up to blastocyst, which indicated that embryo-derived LIF can act in an autocrine manner on differentiation process and blastocyst formation. This study indicated that the addition of LIF to the embryo culture medium improved development of blastocysts, functional (hatching) and morphological (number of cells) quality of the blastocysts produced in vitro. The stage-specific expression pattern of LIF and LIFRβ mRNA transcripts in buffalo embryos indicated that LIF might play an important role in the preimplantation development and subsequent implantation of buffalo embryos.

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Section: Discussionsupporting

confidence: 77%

Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRβ) in buffalo preimplantation embryos produced in vitro: markers of successful embryo implantation

Eswari

Kumar

Sharma

2012

Zygote

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“…Vero cell secretions include the growth factors PDGF, IL-6 and LIF [31], all of which are also found in the reproductive tract [36][37][38][39]. An interesting study by Carnegie et al suggests that in vitro production of blastocysts with high cryotolerance can be modulated by levels of LIF secretion by Vero cells [40].…”

Section: Discussionmentioning

confidence: 99%

Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect post-thaw development and apoptosis in cryopreserved embryos

Desai

Kattal

AbdelHafez

et al. 2007

J Assist Reprod Genet

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“…When the maturation of bovine oocytes was stimulated, the development of embryos accelerated and their morphological quality was improved, which resulted in a higher yield of embryos suitable for cryopreservation (de Matos et al, 1996;Mtango et al, 2003). Culture conditions have been shown to be a very important factor for survival of embryos after cryopreservation (Carnegie et al,1999;Hochi et al, 1999;Otoi et al, 2000;Cho et al, 2002;Abe and Hoschi 2003;Galli et al, 2003;. It could be shown that, that it is possible to obtain more developed embryos of higher morphological quality that were more resistant to the cryopreservation procedure by modification of the culture condition.…”

mentioning

confidence: 99%

Collection of oocytes from donors in the growth phase of follicular development can enhance the production of bovine embryos for cryopreservation

Machatkova¹,

Hanzalova²,

Horakova³

et al. 2006

Vet. Med. - Czech

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232The application of biotechnology in cattle breeding has allowed us to reduce the generation interval and to increase selection pressure in animal populations on the basis of more objective evaluation of the genotype. It facilitates more effective utilization of the reproductive potential of animals, makes a broader range of parental combinations available and thus allows for modification of the animal population in a desired way.The prerequisite for achieving these goals involves efficient cryopreservation methods for long-term storage of embryos, exchange of genetic material and creation of genetic resources. Today the embryos obtained by superovulation can be fro- ABSTRACT: The present study was designed to compare the efficiency of bovine embryo production for cryopreservation between oocytes collected from donors in the growth phase of follicular development (GPFD) and those recovered from donors in the undefined phase (UPFD). Cyclic cows, Czech Siemental or Holstein dairy breeds, 4-6 years of age, slaughtered at the local abbatoir were used. They were divided into two groups based on ovarian morphology: I. GPFD donors with ovaries corresponding to the growth phase of the first follicular wave (estrus cycle days 3-4; n = 52), and II. UPFD donors with ovaries in any other phase of follicular development (undefined estrus cycle days; n = 89). A total of 3 771 oocytes were collected and 1 134 embryos were prepared as two separate populations by standard protocol. In total 352 excellent or good quality embryos at the early, advanced or expanded blastocyst stage from both donor groups were pooled and used for cryotolerance assessment. They were frozen on day 7 (D7) or day 8 (D8) after fertilization by the standard procedure. After thawing, the embryos were cultured for 72 h to the hatching stage. The percentages of both D7 embryos and advanced blastocysts were significantly higher (P ≤ 0.01) for oocytes collected from GPFD donors than for oocytes collected from UPFD donors (33.7 vs 28.6% and 43.0 vs 29.5%, respectively). The percentages of excellent or good quality embryos obtained from both D7 embryos and fertilized oocytes were significantly higher (P ≤ 0.01) for oocytes collected from GPFD donors than for oocytes collected from UPFD donors (63.6 vs 49.4% and 21.4 vs 14.1%, respectively). The post-thaw survival rates were significantly higher (P ≤ 0.01) for D7 than D8 embryos (80.4 vs 66.3%). In relation to the developmental stage, the development and hatching rates were significantly higher (P ≤ 0.01) for D7 than D8 early blastocysts (75.0 vs 41.2% and 50.0 vs 5.9%, respectively) and for D7 than D8 advanced blastocysts, (73.7 vs 57.1 and 52.6 vs 28.6%, respectively). No differences were found between D7 and D8 expanded blastocysts after freezing-thawing. In conclusion, the collection of oocytes from donors in the growth phase positively influenced the in vitro production of bovine embryos for cryopreservation. The development of embryos produced with oocytes from GPFD donor group was accelerated and more ex...

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Influence of protein supplements on the secretion of leukaemia inhibitory factor by mitomycin-pretreated Vero cells: possible application to the in vitro production of bovine blastocysts with high cryotolerance

Cited by 17 publications

References 3 publications

Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRβ) in buffalo preimplantation embryos produced in vitro: markers of successful embryo implantation

Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRβ) in buffalo preimplantation embryos produced in vitro: markers of successful embryo implantation

Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect post-thaw development and apoptosis in cryopreserved embryos

Collection of oocytes from donors in the growth phase of follicular development can enhance the production of bovine embryos for cryopreservation

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