Influence of protein sources on performance and plasma amino acid patterns of ruminants

Importance of the Isovalerate Carboxylation Pathway of Leucine Biosynthesis in the Rumen

“…Neutral and acidic amino acids in the protein hydrolysates were separated by column chromatography on ion-exchange resins by use of methods similar to those of Moore et al (29) and Zacharius and Talley (44). Basic amino acids were separated by use of a method similar to that described by Theurer (38).…”