Influence of Preservatives and Topical Steroids on Ciliary Beat Frequency In Vitro

Hofmann, Thiemo; Gugatschga, Markus; Koidl, Bernd; Wolf, Gerald

doi:10.1001/archotol.130.4.440

Cited by 38 publications

(44 citation statements)

References 23 publications

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“…Although a long application history has demonstrated both safety and effectiveness of these two preservatives [6], several studies have demonstrated that BKC impairs the ciliary function of nasal epithelial cells in vitro [7,8,9]. The findings from our present study are in accordance with the findings from other studies employing an in vitro nasal epithelial cell culture model [8,9], and suggest that BKC produces adverse effects on ciliary activity in primary nasal epithelial cell cultures at the concentration commonly used in clinical preparations. In contrast, PS at a clinically employed concentration appears to be a relatively safe alternative with respect to ciliary activity of nasal cells.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Topical Corticosteroids, Topical Antihistamines, and Preservatives on Human Ciliary Beat Frequency

Jiao

Meng

Zhang³

2014

ORL

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Background: The aim of this study was to investigate the effect of the corticosteroids, the antihistamines, and the preservatives benzalkonium chloride (BKC) and potassium sorbate (PS) in intranasal medications on human nasal epithelial ciliary beat frequency (CBF). Methods: Primary ciliated epithelial cell cultures from the human nasal mucosa of chronic sinusitis patients were established. Changes in CBF of epithelial cell cultures treated/untreated with intranasal medications or preservatives were assessed using high-speed digital imaging methods. Results: Budesonide caused a rapid but reversible ciliostasis and showed no ciliotoxic effect at 10% dilution. Fluticasone propionate induced an irreversible ciliostatic activity and showed a reversible decrease in CBF at 10% dilution. Azelastine hydrochloride and levocabastine hydrochloride both induced a dose-dependent and irreversible decrease in CBF, although the ciliotoxic effect was not evident at 5% dilution. BKC resulted in an irreversible ciliostasis at 0.005 or 0.01% concentrations, whereas PS did not show any change in CBF at 0.12 or 0.24% concentrations. Conclusions: Crystalline BKC and BKC-containing intranasal medications, including fluticasone propionate, azelastine hydrochloride and levocabastine hydrochloride, but not PS or PS-containing intranasal budesonide spray, led to irreversible ciliostasis in human nasal epithelial cell cultures when applied at clinically relevant concentrations.

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Section: Discussionmentioning

confidence: 99%

The Effect of Topical Corticosteroids, Topical Antihistamines, and Preservatives on Human Ciliary Beat Frequency

Jiao

Meng

Zhang³

2014

ORL

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“…In normal human subjects, the treatment with FP apparently potentiates mucin secretion while not changing serous secretion or microvascular permeability (26). On the other hand, FP increases sodium absorption as measured by changes in the voltage across the epithelium (17) and slows the ciliary beat frequency (19). It is possible that these effects on mucus and ciliary movements would affect olfaction as well.…”

Section: Discussionmentioning

confidence: 99%

The olfactory system is affected by steroid aerosol treatment in mice

Mucignat‐Caretta¹,

Bondı̀²,

Rubini³

et al. 2009

American Journal of Physiology-Lung Cellular and Molecular Phys

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Asthma needs continuous treatment often for years. In humans, some drugs are administered via aerosol, therefore they come in contact with both respiratory and olfactory mucosa. We explored the possibility that antiasthma corticosteroid treatment could influence the olfactory function by passage through the nose. A group of mice was exposed twice daily for 42 days to fluticasone propionate aerosol and was compared with a control group. Olfactory behavior, respiratory mechanics, histology, and immunoreactivity in the olfactory system were assessed. Fluticasone-treated mice were slower in retrieving a piece of hidden food, but both groups were similarly fast when the food was visible. When a clearly detectable odor was present in the environment, all mice behaved in a similar way. Respiratory mechanics indices were similar in all mice except for the viscose resistance, which was reduced in fluticasone-treated mice. Olfactory mucosa of fluticasone-treated mice was thicker than that of controls. Slight but consistent differences in staining were present for Olfactory Marker Protein but not for other proteins. A mild impairment of olfactory function is present in mice chronically treated with fluticasone aerosol, apparently accompanied by slight modifications of the olfactory receptor cells, and suggests monitoring of olfactory function modifications in long-term steroid users.corticosteroid; behavior THE OLFACTORY SYSTEM IS CHARACTERIZED by a rapid turnover of receptor neurons, for which lifespan is ϳ1 mo, and a neuron turnover in the first relay station, the olfactory bulb (OB). The receptor axons make synapses with dendrites of mitral cells in the glomeruli (11). OB interneurons are continuously renewed by cells that differentiate from neural stem cells residing in the subventricular zone. They arrive into the OB via the rostral migratory stream and are subsequently integrated in OB circuits (25, 33). Other chemosensory organs are present in the nasal cavity, concurring to chemosensory perception (8,39). This dynamic network is sensitive to environmental challenges, which often result in mucosal damage that is subsequently reflected in OB, affecting olfactory sensitivity (9, 29).Pathological conditions of the airways may affect the olfactory performance. Both upper and lower airways diseases, for example allergic rhinitis and asthma, are treated with topical administration of corticosteroids. Fluticasone propionate (FP) is one of the most popular and safe treatments for both conditions (2) due to its hepatic metabolism that allows only a topic anti-inflammatory effect without adrenal suppression (31). Whereas the improvement in the pathological state is well-documented (in humans, see, for example, Refs. 13,24,30,34,37; in mice, Refs. 12,32), little is known about its possible effect on olfactory cells and in the subsequent olfactory performance.Corticosteroid inhalatory treatments are often long-lasting, therefore they may influence the quality of life of patients even if they induce only slight side effect. Inha...

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“…Ряд исследований показал, что нанесение бензалкония хлорида на слизистую оболочку полости носа вызы вает раздражение, гиперсекрецию и замедление му коцилиарного транспорта [28,29]. Ощущение жже ния и явления раздражения в полости носа были значительно сильнее у пациентов, которые получа ли оксиметазолин с добавлением консерванта (бен залкония хлорида), и статистически значимо умень шались у больных, получавших препарат в чистом виде [30][31][32].…”

Section: фармакотерапия заболеваний носа и околоносовых пазух российсunclassified

Topical nasal decongestants: Comparative characteristics and a review of side effects

Varvyanskaya¹,

Лопатин²

2015

Ross. rinol.

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Influence of Preservatives and Topical Steroids on Ciliary Beat Frequency In Vitro

Cited by 38 publications

References 23 publications

The Effect of Topical Corticosteroids, Topical Antihistamines, and Preservatives on Human Ciliary Beat Frequency

The Effect of Topical Corticosteroids, Topical Antihistamines, and Preservatives on Human Ciliary Beat Frequency

The olfactory system is affected by steroid aerosol treatment in mice

Topical nasal decongestants: Comparative characteristics and a review of side effects

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