Influence of Preadsorbed Block Copolymers on Protein Adsorption: Surface Properties, Layer Thickness, and Surface Coverage

Stuffed brushes: theory and experiment

“…In another study PEO was fixed to an alkyl chain and an increasing PEO length was found to strongly reduce the protein adsorption [7]. The PEO chain length was 128 or less for McPherson et al [3] and Schoën et al [4] and 17 or less units in the case of Prime et al [7].…”

“…Protein adsorption can result in coagulation of adsorbed blood proteins and subsequent surface-induced thrombosis [1]. Grafting or irreversible adsorption of watersoluble polymers onto the surface is a well-established method to reduce protein adsorption [2][3][4][5][6][7]. The decrease in the adsorbed amount of protein is generally attributed to steric repulsion between the grafted hydrophilic polymer and the solvated proteins.…”

“…In recent studies the adsorption of several proteins on a hydrophobic surface was measured as a function of PEO-PPO-PEO triblock copolymer surface density [3,4]. It was shown that an increase in surface density results in a continuous decrease in protein adsorption.…”

Adsorption of a PEO–PPO–PEO triblock copolymer on metal oxide surfaces with a view to reducing protein adsorption and further biofouling

“…At this stage, it is assumed that Plu forms a brush structure with relatively high density on Ti/OPA and SS/OPA, which prevents further Plu molecules to approach (Nejadnik et al 2009). It has been reported (Nejadnik et al 2009) that PEO-PPO-PEO can form a brush structure on hydrophobic surfaces with WCA above 80°, which is then effective to reduce protein adsorption (Schroen et al 1995). In contrast, PEO-PPO-PEO can form a pancake structure on hydrophilic surfaces (Schroen et al 1995), which is insufficient 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 (Chang et al 2010).…”

“…It has been reported (Nejadnik et al 2009) that PEO-PPO-PEO can form a brush structure on hydrophobic surfaces with WCA above 80°, which is then effective to reduce protein adsorption (Schroen et al 1995). In contrast, PEO-PPO-PEO can form a pancake structure on hydrophilic surfaces (Schroen et al 1995), which is insufficient 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 (Chang et al 2010). The mechanism of protein resistance ability of PEO-PPO-PEO is attributed to the high hydration of PEO chains extending into the bulk solution, resulting in a steric barrier (Freij-Larsson et al 1996;Green et al 1998).…”