Influence of Pre-analytical Variables on VEGF Gene Expression and Circulating Protein Concentrations

Azimi‐Nezhad, Mohsen; Lambert, Daniel; Ottone, Catherine; Perrin, Corinne; Chapel, Celine; Gaillard, Gwenaëlle; Pfister, Michèle; Masson, Christine; Tabone, Eric; Betsou, Fay; Meyronet, David; Ungeheuer, Marie‐Noëlle; Visvikis‐Siest, Sophie

doi:10.1089/bio.2012.0016

Cited by 15 publications

(31 citation statements)

References 41 publications

(43 reference statements)

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“…The individual data records are pseudoanonymized according to the European (GDPR directive) and French (CNIL) laws in order to protect the participants' privacy. Sample quality controls are regularly performed following a biospecimen research schedule [8,18,23].…”

Section: Sample Managementmentioning

confidence: 99%

“…The Diagmicoll main cohort (Clinical trials reference: NTC03912246) affords bio-resources from healthy subjects primarily, for the National Reference Centers, so as to optimize and develop new diagnostic methods [1,3,4,5,8,10,13,20,21,28]. The CoSImmGEn cohort (Clinical trials reference: NTC03925272) has been set up to take in charge more complex needs such as longitudinal studies of the immune system and other integrative (e.g.…”

mentioning

confidence: 99%

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The ICAReB Platform: A Human Biobank for the Institut Pasteur and Beyond

Esterre

Ait-Saadi

Arowas

et al. 2020

Open Journal of Bioresources

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The ICAReB platform of Institut Pasteur provides access to human bio-resources for academic and private research teams worldwide, essentially in the fields of infection, immunity and neurosciences. More than 134,000 human quality controlled, duly annotated samples (mainly whole-blood derived products, but also stool, urine, saliva, swabs … .), from both healthy and diseased cohorts with open, regulated access, are available upon request. Both clinical investigation and biobanking activities are certified following ISO 9001:2015 and NF S96-900:2011 standards, respectively. ICAReB is a member of PIBnet (Pasteur International Biobanking network), BIOBANQUES and BBMRI, the French and pan-european biobanking networks, respectively.

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Section: Sample Managementmentioning

confidence: 99%

mentioning

confidence: 99%

The ICAReB Platform: A Human Biobank for the Institut Pasteur and Beyond

Esterre

Ait-Saadi

Arowas

et al. 2020

Open Journal of Bioresources

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“…In terms of protein preservation, there is variability for individual proteins. In muscle biopsies, vascular endothelial growth factor protein expression tended to be higher in specimens subjected to a 30 or 60 minutes cold ischemia time compared to those frozen within 15 minutes of tissue removal [38]. Significant reduction in immunohistochemistry (IHC) staining for progesterone receptor in breast cancer samples occurs after 2 hours for non-refrigerated samples [39].…”

Section: Considerations In the Procurement Of Tissue And Blood Biomentioning

confidence: 99%

“…Baumann et al reported that the best reproducibility of proteomic patterns is with a single thawing of frozen serum samples and that repeated freeze-thaw cycles lead to significant changes of serum and plasma proteomes [72]. Vascular endothelial growth factor (VEGF) levels in plasma significantly changed with the number of freeze-thaw cycles [38, 73]. Hsieh and colleagues demonstrated that repeated freezing at −80°C and thawing on ice for up to ten times did not present any obvious changes in low-molecular-weight serum and plasma proteomes [45].…”

Section: Freeze-thaw Cycles and Their Effect On Biospecimen Qualitymentioning

confidence: 99%

“…Therefore, assessing protein integrity is important since variation in pre-analytical conditions may result in proteolysis, protein degradation, or other proteome-associated changes [38, 72, 98]. There is not currently a cost effective, facile, and comprehensive way to assess protein quality in frozen biospecimens [53].…”

Section: Quality Control and Quality Control Toolsmentioning

confidence: 99%

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The procurement, storage, and quality assurance of frozen blood and tissue biospecimens in pathology, biorepository, and biobank settings

Shabihkhani

Lucey

Wei

et al. 2014

Clinical Biochemistry

212

182

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Well preserved frozen biospecimens are ideal for evaluating the genome, transcriptome, and proteome. While papers reviewing individual aspects of frozen biospecimens are available, we present a current overview of experimental data regarding procurement, storage, and quality assurance that can inform the handling of frozen biospecimens. Frozen biospecimen degradation can be influenced by factors independent of the collection methodology including tissue type, premortem agonal changes, and warm ischemia time during surgery. Rapid stabilization of tissues by snap freezing immediately can mitigate artifactually altered gene expression and, less appreciated, protein phosphorylation profiles. Collection protocols may be adjusted for specific tissue types as cellular ischemia tolerance varies widely. If data is not available for a particular tissue type, a practical goal is snap freezing within 20 minutes. Tolerance for freeze-thaw events is also tissue type dependent. Tissue storage at −80°C can preserve DNA and protein for years but RNA can show degradation at 5 years. For −80°C freezers, aliquots frozen in RNAlater or similar RNA stabilizing solutions is a consideration. It remains unresolved as to whether storage at −150°C provides significant advantages relative to −80°C. Histologic quality assurance of tissue biospecimens is typically performed at the time of surgery but should also be conducted on the aliquot to be distributed because of tissue heterogeneity. Biobanking protocols for blood and its components are highly dependent on intended use and multiple collection tube types may be needed. Additional quality assurance testing should be dictated by the anticipated downstream applications.

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A Practical Approach to Clinical and Research Biobanking

Yong¹,

Dry

Shabihkhani

2014

Methods in Molecular Biology

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Powerful technologies critical to personalized medicine and targeted therapeutics require the analysis of carefully validated, procured, stored, and managed biospecimens. Reflecting advancements in biospecimen science, the National Cancer Institute and the International Society for Biological and Environmental Repositories are periodically publishing best practices that can guide the biobanker. The modern biobank will operate more like a clinical laboratory with formal accreditation, standard operating procedures, and quality assurance protocols. This chapter highlights practical issues of consent, procurement, storage, quality assurance, disbursement, funding, and space. Common topics of concern are discussed including the differences between clinical and research biospecimens, stabilization of biospecimens during procurement, optimal storage temperatures, and technical validation of biospecimen content and quality. With quickly expanding biospecimen needs and limited healthcare budgets, biobanks may need to be selective as to what is stored. Furthermore, a shift to room-temperature storage modalities where possible can reduce long-term space and fiscal requirements.

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Influence of Pre-analytical Variables on VEGF Gene Expression and Circulating Protein Concentrations

Cited by 15 publications

References 41 publications

The ICAReB Platform: A Human Biobank for the Institut Pasteur and Beyond

The ICAReB Platform: A Human Biobank for the Institut Pasteur and Beyond

The procurement, storage, and quality assurance of frozen blood and tissue biospecimens in pathology, biorepository, and biobank settings

A Practical Approach to Clinical and Research Biobanking

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