Influence of Polyvinylpyrrolidone Concentration on Properties and Anti-Bacterial Activity of Green Synthesized Silver Nanoparticles

Abstract

Environmentally green synthesis of stable polyvinyl pyrrolidone (PVP)-capped silver nanoparticles (PVP-AgNPs) was successfully carried out. The present study focused on investigating the influence of adding PVP during the synthesis process on the size, optical properties and antibacterial effect of silver nanoparticles produced. An aqueous extract of Eucalyptus camaldulensis leaves was used as a reducing agent. The effects of different PVP concentrations and reducing time on the synthesis of nanoparticles (NPs) were characterized by UV–Vis spectrophotometry, scanning electron microscopy (SEM), energy dispersive spectrum (EDX), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and nano tracker analysis (NTA). The addition of PVP was studied. The prepared PVP-AgNPs were spherical with an average size of 13 nm. FTIR analysis confirmed that PVP protects AgNPs by a coordination bond between silver nanoparticles and both N and O of PVP. DLS results indicated the good dispersion of silver nanoparticles. PVP-AgNPs were found to be stable for nearly 5 months. Antibacterial studies through the agar well diffusion method confirmed that silver nanoparticles synthesized using PVP had no inhibitor activity toward Gram-positive and Gram-negative bacteria as opposed to silver nanoparticles prepared without adding PVP, which showed a significant antibacterial activity towards some of the tested pathogens.

A Novel Based Synthesis of Silver/Silver Chloride Nanoparticles from Stachys emodi Efficiently Controls Erwinia carotovora, the Causal Agent of Blackleg and Soft Rot of Potato

“…NPs synthesized by using Eucalyptus camaldulensis were tested against various bacteria and were found to significantly reduce the Gram-negative bacteria growth. The antibacterial character of Ag/AgClNPs prepared from E. camaldulensis could be ascribed to the small particle size and high surface-to-volume ratio, which let the nanoparticles interact with bacterial membranes [ 61 ]. According to a proposed mechanism which describes how silver particles act, due to their small size and spherical shape, they can penetrate bacterial cell walls and can increase their permeability by bringing some structural changes; these changes include the generation of pores in the bacterial cell wall through reactive oxygen species production.…”

Biodegradable nanocomposites prepared from poly(butylene adipate terephthalate) and silver nanoparticles for applications as disposable medical equipments

“…After addition of AgNPs, some changes are observed in the position and intensity of the peaks. The stretching peaks of CO and CN shift to 1655 and 1020 cm −1 for PBAT/AgNPs 0.5 , and to 1650 and 1018 cm −1 for PBAT/AgNPs 1.0 , which can be attributed to the interaction or coordination between AgNPs and nitrogen‐ or oxygen‐containing groups of PVP 27 …”

“…As shown in Figure 4, the surface of neat PBAT, PBAT/PVP, PBAT/AgNPs 0.5 , and PBAT/AgNPs 1.0 films all appeared rather smooth. AgNPs were observed on the surface of PBAT/AgNPs composite films with some aggregation, especially in the case of PBAT/AgNPs 1.0 27 . More aggregation is detected in PBAT/AgNPs 1.0 than in PBAT/AgNPs 0.5 probably due to uneven dispersion of high concentration AgNPs in the PBAT matrix 28 …”

The Release of Non-Extractable Ferulic Acid from Cereal By-Products by Enzyme-Assisted Hydrolysis for Possible Utilization in Green Synthesis of Silver Nanoparticles

“…The presence of C (carbon) as the second prevalent element observed along with Ag is conditioned by the double-sided adhesive carbon disc used for the fixation of AgNPs. It has been proposed that amphiphilic PVP can act as stabilizing and/or reducing agents alone or in combination with specific functional molecules and promote the formation of NPs with a narrow particle size distribution of an average of 17 nm [ 52 ]. This statement has also been reinforced by this study’s results, by observing the homogeneous shapes and substantially smaller size of PVP capped AgNPs corresponding to an average size of 22.4 ± 7.0 nm.…”