Influence of polyamines on DNA binding of heat shock and activator protein 1 transcription factors induced by heat shock

Desiderio, Maria Alfonsina; Dansi, Paola; Tacchini, Lorenza; Bernelli‐Zazzera, Aldo

doi:10.1016/s0014-5793(99)00873-x

Cited by 21 publications

(16 citation statements)

References 34 publications

(31 reference statements)

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“…It is well known that the physiological-biochemical activity of di-and polyamines is mainly due to their polycation structure. In all probability, the more number of amine groups in poliamine, the more metabolically active this compound is, because in this way it possesses greater biochemical possibilities to interact with nucleic acids, proteins, hormones and another metabolites characterised by regulating function (Van den Broeck et al 1994;Davis 1997;Tiburcio et al 1997;Laitinen et al 1998;Desideiro et al 1999;Kotzabasis et al 1999). From the studies carried out on the C. vulgaris it was seen that the highest biological activity of spermidine results from the fact that it contains three amine groups of a cation character whereas the lowest activity is noted in agmatine with only one -NH 2 group.…”

Section: Resultsmentioning

confidence: 99%

“…From publications Shih et al 1982;Smith 1985;Gniazdowska-Skoczek et al 1994;Ye et al 1994;Bajguz and Czerpak 1999;Desideiro et al 1999) it is known that di-and polyamines stimulate the synthesis of ribonucleic acids and proteins by activation of the polymerase of RNA and synthase of aminoacyl-tRNA and stabilisation of ribosomes. Di-and polyamines delay the process of ageing in plants mainly by inhibiting ethylene biosynthesis and weakening the activity of the enzymes: ribonuclease, protease, hydrolase, oxidase and others, which degrade metabolites important for the life of the plant.…”

Section: Resultsmentioning

confidence: 99%

“…In chloroplasts di-and polyamines stimulate the photosynthetic process in both its phases the dark and the light as well as activating the primary and secondary growth of the cell wall. These compounds stimulate the active transport of metabolites, the functioning of enzymes and ion pumps localised in the cellular membranes Shih et al 1982;Smith 1985;Pjon et al 1990;Gniazdowska-Skoczek et al 1994;Bagni 1997;Kakkar et al 1997/98;Laitinen et al 1998;Bajguz and Czerpak 1999;Czerpak and Bajguz 1999;Desideiro et al 1999;Kotzabasis et al 1999).…”

Section: Introductionmentioning

confidence: 99%

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Biochemical activity of di- and polyamines in the green alga Chlorella vulgaris Beijerinck (Chlorophyceae)

et al. 2011

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This study concerns on the influence of diamines (agmatine, putrescine) and polyamines (spermine, spermidine) upon the growth and the content of chlorophyll a and b, monosaccharides and proteins in the cells of alga Chlorella vulgaris Beijerinck (Chlorophyceae). In the experiments agmatine, putrescine, spermine and spermidine in the range of concentrations 10 -6 -10 -3 M were used. At the concentration 10 -3 M and the 1st day of cultivation, they have a toxic effect on growth of the algae. It was found that di-and polyamines used within the range of concentration 10 -6 -10 -4 M stimulate the growth and the contents of analysed biochemical parameters in the cells of C. vulgaris. The most stimulating influence on metabolism of the alga was demonstrated by spermidine and putrescine at concentration of 10 -4 M. Agmatine and spermine were characterised by a lower biological activity than spermidine and putrescine demonstrated the most stimulating influence.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biochemical activity of di- and polyamines in the green alga Chlorella vulgaris Beijerinck (Chlorophyceae)

et al. 2011

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“…Polyamines have been shown to enhance the binding of several gene regulatory proteins, including NF-IL6, USF, Ig/EBP and ERa to DNA (Panagiotidis et al, 1995;Thomas et al, 1995). In contrast, STAT3 provides an example of a transcription factor binding insensitive to changes in polyamine levels (Desiderio et al, 1999), while Oct-1 binding to DNA was inhibited by increased polyamines (Panagiotidis et al, 1995). Synthetic polyamine analogs disrupted ERa and NF-kB transactivation, suggesting a possible pathway for the growth inhibitory and apoptosis-inducing e ects of polyamine analogs on breast cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Regulation of estrogenic and nuclear factor κB functions by polyamines and their role in polyamine analog-induced apoptosis of breast cancer cells

Shah

Thomas

Lewis³

et al. 2001

Oncogene

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The natural polyamines -putrescine, spermidine, and spermine-are essential for cell growth and di erentiation. Polyamines are involved in several gene regulatory functions, although their mechanism(s) of action has not been elucidated. We investigated the role of polyamines in the function of NF-kB and estrogen receptor-a (ERa), two transcription factors implicated in breast cancer cell proliferation and cell survival, using MCF-7 breast cancer cells. We found that spermine facilitated the binding of ERa and NF-kB to estrogen response element (ERE)-and NF-kB response element (NRE), respectively, and enhanced ERa-mediated transcriptional activation in transient transfection experiments. We also found that the association of the co-regulatory protein CBP/p300 with ERa and NF-kB was increased by spermine treatment of MCF-7 cells. Spermine also increased the nuclear translocation of NF-kB compared to the control. In contrast, treatment of MCF-7 cells with polyamine analogs, BE-3-4-3 and BE-3-3-3, resulted in transcriptional inhibition of both ERE-and NRE-driven reporter plasmids. In addition, polyamine analogs inhibited the association of ERa and NF-kB with CBP/p300 and were unable to facilitate nuclear translocation of NF-kB. APO-BRDU assay demonstrated that polyamine analogs induced apoptosis, with a loss of the anti-apoptotic protein Bcl-2. These data show a gene regulatory function of polyamines involving transcriptional activation of ERa and NF-kB, potentially leading to the up-regulation of genes involved in breast cancer cell proliferation. Our results with BE-3-4-3 and BE-3-3-3 suggest that down-regulation of ERa-and NF-kB-regulated genes is a possible mechanism for the action of polyamine analogs in inducing apoptosis of breast cancer cells. Oncogene (2001) 20, 1715 ± 1729.

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“…Polyamines not only stabilize cellular nucleic acids and membranes, but also play a pivotal role in the regulation of gene expression both by altering DNA structure and by modulating protein kinases and transcription factors (2)(3)(4)(5). When young, growing cells have increased polyamine synthesis and higher intracellular concentration; however, with aging, the enzymatic activities for polyamine synthesis and the ability to synthesize polyamine decrease (6,7).…”

mentioning

confidence: 99%

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

Soda¹,

Kano²,

Nakamura³

et al. 2005

The Journal of Immunology

113

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Natural polyamines, spermine, spermidine, and putrescine, play a pivotal role in the regulation of gene expression; therefore, the age-dependent decreases and the disease-dependent increases in polyamine synthesis suggest a possible contribution of polyamines to the age-related and disease-associated changes in cellular function. In this study, we examined the effects of polyamines on the cellular function and the expression of adhesion molecules on human PBMCs from healthy volunteers. Flow cytometry revealed that PBMCs cultured with spermine decreased mean fluorescent intensities (MFIs) of CD11a and CD18 in the lymphocyte light-scattered region, but not in the monocyte region. This suppression was observed in a dose- and time-dependent manner and found nonspecifically on all cell subsets we tested (CD3+, CD4+, CD8+, CD19+, CD45RA+, CD45RO+, CD4+CD45RA+, CD4+CD45RO+, CD8+CD45RA+, CD8+CD45RO+). The decreases of CD11a and CD18 MFIs were accompanied by the decrease in adherent capacity of PBMCs to HUVECs. Spermine did not hinder cell activities or cell viability. Among 42 healthy volunteers (mean, 49.5 years old; from 26 to 69), blood spermine levels inversely correlated with the CD11a MFIs of cells in the lymphocyte region (r = −0.48; p = 0.001), but not with those in the monocyte region. The effects of spermidine seemed weaker than those of spermine, and blood spermidine levels had no correlation with CD11a MFIs of the lymphocyte region. Putrescine had no effect on the expressions of membrane molecules. Polyamines, especially spermine, decrease LFA-1 (CD11a/CD18) expression on human lymphocyte and adhesion capacity of PBMCs to HUVECs.

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Influence of polyamines on DNA binding of heat shock and activator protein 1 transcription factors induced by heat shock

Cited by 21 publications

References 34 publications

Biochemical activity of di- and polyamines in the green alga Chlorella vulgaris Beijerinck (Chlorophyceae)

Biochemical activity of di- and polyamines in the green alga Chlorella vulgaris Beijerinck (Chlorophyceae)

Regulation of estrogenic and nuclear factor κB functions by polyamines and their role in polyamine analog-induced apoptosis of breast cancer cells

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

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