Influence of Podophyllum hexandrum on endogenous antioxidant defence system in mice: possible role in radioprotection

Mittal, Anshu; Pathania, V.; Agrawala, Paban K.; Prasad, Jagdish; Singh, Satish Kumar; Goel, Harish Chandra

doi:10.1016/s0378-8741(01)00243-4

Cited by 59 publications

(35 citation statements)

References 5 publications

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“…In this study, peroxiredoxin I and glutathione Stransferase P2 were up-regulated by ionizing radiation, while antioxidant protein 2 was down-regulated. These proteins have been demonstrated to be involved in radiation [24][25][26] . Peroxiredoxin I is involved in the redox regulation of cells, such as reducing peroxides with reducing equivalents through the thioredoxin system but not from glutaredoxin.…”

Section: Discussionmentioning

confidence: 99%

Differentially expressed proteins of gamma-ray irradiated mouse intestinal epithelial cells by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

Zhang

Su²,

Ai³

et al. 2003

WJG

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AIM:To identify the differentially expressed proteins involved in ionizing radiation in mice and to explore new ways for studying radiation-related proteins. METHODS:Bal B/c mice grouped as sham-irradiation, 3 h and 72 h irradiation were exposed to 9.0Gy single dose of γ-irradiation. Intestinal epithelia were isolated from mice, and total proteins were extracted with urea containing solution. A series of methods were used, including twodimensional electrophoresis, PDQuest 2-DE software analysis, peptide mass fingerprinting based on matrixassisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and SWISS-PROT database searching, to separate and identify the differential proteins. Western blotting and RT-PCR were used to validate the differentially expressed proteins. RESULTS:Mouse intestine was severely damaged by 9.0 Gy γ-irradiation. Image analysis of two-dimensional gels revealed that averages of 638±39, 566±32 and 591±29 protein spots were detected in 3 groups, respectively, and the majority of these protein spots were matched. About 360 protein spots were matched between normal group and 3 h irradiation group, and the correlation coefficient was 0.78 by correlation analysis of gels. Also 312 protein spots matched between normal group and 72 h irradiation group, and 282 protein spots between 3 h and 72 h irradiation groups. Twenty-eight differential protein spots were isolated from gels, digested with trypsin, and measured with MALDI-TOF-MS. A total of 25 spots yielded good spectra, and 19 spots matched known proteins after database searching. These proteins were mainly involved in anti-oxidation, metabolism, signal transduction, and protein post-translational processes. Western-blotting confirmed that enolase was up-regulated by γ-irradiation. Upregulation of peroxiredoxin I was verified by applying RT-PCR technique, but no change occurred in Q8VC72. CONCLUSION:These differentially expressed proteins might play important roles when mouse intestine was severely injured by γ-irradiation. It is suggested that differential proteomic analysis may be a useful tool to study the proteins involved in radiation damage of mouse intestinal epithelia.

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Section: Discussionmentioning

confidence: 99%

Differentially expressed proteins of gamma-ray irradiated mouse intestinal epithelial cells by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

Zhang

Su²,

Ai³

et al. 2003

WJG

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“…[70] LPO products such as MDA form adducts with cellular DNA. [71] Oxidative stress results in imbalance between radical generating and radical scavenging ability in cells leading to cell membrane or DNA damage. [72] It was suggested that ROS related DNA damage led to formation of single and double stranded breaks resulting in cell cycle arrest.…”

Section: World Journal Of Pharmacy and Pharmaceutical Sciencesmentioning

confidence: 99%

Effect of Plant Hormone Gibberellic Acid-3 on Rat Testicular Biomarkers and Its Histology

Suriamurthy¹

2017

WJPPS

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“…In the treatment of leukemia, lung and testicular cancer, psoriasis, malaria, rheumatoid arthritis podophyllotoxin has been used as a precursor of topoisomerase inhibitor. In the last couple of decades many radioprotective studies using P. hexandrum has been performed such as survival studies, gastroprotection, neuroprotection, hepatoprotection, hematopoietic system protection in different strain of mice against gamma radiation 35 .…”

Section: Podophyllum Hexandrummentioning

confidence: 99%

Radiation Countermeasures: Current Status

et al. 2017

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Deleterious effects of ionising radiation leading to significant morbidity and mortality have been studied elaborately. A range of synthetic, semisynthetic and herbal compounds have been screened as radiation countermeasure agents and a number of promising radiation countermeasure agents are under development. Amifostin is the only drug which has been approved by the United State Food and Drug Administration (US-FDA), but that too for use in a defined population under strict medical supervision. Granulocyte Colony Stimulating Factor/filgrastim, γ-tocotrienol, genistein are at an advanced stage of development and are tested on higher animal models as per required norms of FDA. Herbal compounds are also considered very safe and of high value as radiation countermeasure agents owing to various properties like antioxidant, immunomodulation activity etc. Histone deacetylase inhibitors such as Trichostatin A, Diallyl sulphide, Sulforaphane are being viewed as very promising radiomitigating agents by our laboratory and active research in the same direction is going on. Infusion of hematopoietic stem cells and growth factors are in practice as potent therapeutics. This review gives an overview on various radioprotectors, radiomitigators and therapeutic agents either available or under development currently.

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Influence of Podophyllum hexandrum on endogenous antioxidant defence system in mice: possible role in radioprotection

Cited by 59 publications

References 5 publications

Differentially expressed proteins of gamma-ray irradiated mouse intestinal epithelial cells by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

Differentially expressed proteins of gamma-ray irradiated mouse intestinal epithelial cells by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

Effect of Plant Hormone Gibberellic Acid-3 on Rat Testicular Biomarkers and Its Histology

Radiation Countermeasures: Current Status

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