Influence of plasma and ultraviolet treatment of zirconia on initial attachment of human oral keratinocytes: Expressions of laminin γ2 and integrin β4

Abstract

Initial attachment of human oral keratinocytes cultured on yttria-stabilized tetragonal zirconia polycrystal (TZP) surfaces that were subjected to UV or oxygen plasma (O2-plasma) treatment was investigated. The viability of the attached cells, mRNA expression of laminin γ2 and integrin β4, distribution of laminin γ2 and integrin β4, cell area, and cell morphology were assessed. The results showed that no differences in the viability of attached cells were recognized among the conditions. However, expression of laminin γ2 and integrin β4 as well as cell morphology were promoted only in O2-plasma specimens even though superhydrophilicity was obtained in both the UV and O2-plasma specimens compared with the untreated control specimen. The photocatalytic activity was believed to be closely involved in the above-mentioned differences. The results of this study suggest that TZP surface treated with oxygen plasma promotes the initial attachment capability of human oral keratinocytes with enhancing the extracellular matrix such as laminin γ2.

Effects of cleaning methods for custom abutment surfaces on gene expression of human gingival fibroblasts

“…Plasma irradiation increases surface hydrophilicity by reducing the contact angle (23)(24)(25) and increases cell attachment to the irradiated surface.…”

“…Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) assay Cells on the zirconia disks (n = 3) in the control and treatment groups were lysed at each time point (6,24, and 48 h after seeding), to collect mRNA (NucleoSpin RNA, Machery-Nagel, Dueren, German) and convert it to cDNA with reverse transcriptase (GoScript Reverse Transcription System, Promega), in accordance with the manufacturer's protocol. A microvolume UV-vis spectrophotometer (SimpliNano, GE Healthcare Life Sciences, Buckinghamshire, UK) was used to measure absorbance of mRNA and cDNA samples.…”

Surrounding Tissue Response to Surface-Treated Zirconia Implants

“…For example, the oxygen plasma technique is also known to produce a superhydrophilic zirconia surface. Kobune et al [60] reported that oxygen plasma treatment of zirconia promoted the initial attachment of oral keratinocytes by enhancing extracellular matrix components such as laminin γ2 better than UV treatment. Oxygen plasma treatment of blastedHF zirconia implants may provide different results.…”

Effect of Physicochemical Surface Modifications on Bovine Serum Albumin Adsorption to Tetragonal Zirconia Polycrystal <i>in vitro</i> Through the change of the Zeta Potential

“…Superhydrophilic surface treatment enhances the initial attachment of osteoblast-like cells 8 and oral keratinocytes 9 . The initial attachment and colonization of periodontal pathogenic bacteria on the surfaces of TZP were the same as were those on the titanium surfaces.…”