Influence of Piper betle L. extract on umbilical cord cells in vitro and potential treating cutaneous wound

Abstract

This research aimed to test the effects of Piper betle L. from Vietnam on fibroblasts and UC-derived mesenchymal stem cells (UC-MSCs) from human umbilical cord (UC) on the scratch assay. We tested the extract at different concentrations and then assessed the level of expression of the factors involved in the inflammatory process on fibroblasts including IL-33, VCAM, CD248 by assay real time qPCR. At the concentrations of 0.025 μL/mL and 0.03 μL/mL, the extracts positively affected fibroblast proliferation and UC-MSCs. By contrast, the concentration of 0.058 μL/mL, the extract was toxic to UC-MSCs and fibroblast cell lines, the cells were no longer able to survive. qPCR results show that Piper betle L. extract has the ability to reduce the expression levels of IL-33 (50.8%), VCAM (32.1%), CD248 (46.13%) which trigger inflammatory processes, thereby reducing cellular stress and promoting the process of healing scratches. Our study revealed the proliferation capabilities of Piper betle L. extract from Vietnam In vitro. Hence, Piper betle L. could be recommended as a potential source of wound healing agents.

Betel Leaf Oil as a Natural Disinfection Agent in Radiological Equipment (Study of Microbial Quantity of Radiographic Cassette)

“…Based on research from Dartini et al (2017), it was mentioned that using chlorine solution as a disinfection material on radiographic cassettes is effectively 2% better than using alcohol with a content of 70%. In this study, researchers used betel leaf oil with the consideration that it is a natural ingredient, easy to obtain in Indonesia, and the use of local wisdom as a material of value in the community, besides that because it is a natural ingredient, it will not provide side effects in the form of irritation to the skin or damage the surface on the radiographic cassettes (Emrizal et al, 2014;Rahayu et al, 2019;Melgar et al, 2022;Thi et al, 2021).…”