Influence of pigeon interferon alpha (PiIFN-α) on pigeon circovirus (PiCV) replication and cytokine expression in Columba livia

Santos, Harvey M.; Chen, Chia Chi; Tsai, Chin‐Feng; Hsish, Yao Ching; Chung, Feng Chiao; Tyan, Yu‐Chang; Tayo, Lemmuel L.; Chuang, Kui-Hao

doi:10.1016/j.vetmic.2020.108591

Cited by 16 publications

(24 citation statements)

References 52 publications

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“…Moreover, a recent study using pigeon interferon‐alpha ( PiIFN‐α ) has shown the involvement of Mx1 and STAT1 in inhibiting the replication of PiCV (Santos et al . 2020a). Our results demonstrated the upregulation of the transcriptional expression of these genes, which is consistent with previous reports on probiotic strains such as Lactobacillus gasseri (Nakayama et al .…”

Section: Discussionmentioning

confidence: 99%

“…Expression and purification of the recombinant cap protein was performed according to the methods described previously (Santos et al . 2020a). The recombinant cap protein was used in the ELISA to test for anti‐PiCV antibodies in pigeon serum (data not shown).…”

Section: Methodsmentioning

confidence: 99%

“…The protocol for experimental infection with PiCV was modified in accordance with the previous report of Santos et al . (2020a). For the antiviral activity test, 10 pigeons were divided into two groups consisting of five pigeons per group: the control and treated groups.…”

Section: Methodsmentioning

confidence: 99%

“…2018; Santos et al . 2020a). To overcome this situation, an alternative prophylaxis for PiCV and YPDS should be developed.…”

Section: Introductionmentioning

confidence: 99%

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Probiotic supplementation containing Bacillus velezensis enhances expression of immune regulatory genes against pigeon circovirus in pigeons ( Columba livia )

Tsai

Santos

et al. 2020

J Appl Microbiol

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Aims In this study, we aimed to isolate and evaluate the efficacy of Bacillus velezensis as a probiotic and to assess its activity towards pigeons infected with pigeon circovirus (PiCV). Methods and Results Bacillus velezensis, isolated from pigeon faeces, was orally administered to pigeons for 60 days. After pigeons were challenged with PiCV, the PiCV viral load and expression of indicator genes for innate immunity were detected in spleen tissue and faeces of pigeons. Bacillus velezensis significantly reduced the PiCV viral load in the faeces and spleen of pigeons 5 days post‐challenge (dpc). The mRNA expression levels of treated pigeons showed that interferon‐gamma (IFN‐γ), myxovirus resistance 1 (Mx1), and signal transducers and activators of transcription 1 (STAT1) genes were upregulated, whereas no expression of interleukin‐4 (IL‐4) was detected. Moreover, toll‐like receptor 2 (TLR2) and 4 (TLR4) were significantly upregulated in probiotic‐treated pigeons (P < 0·05). Conclusions This is the first report showing that probiotic supplementation can effectively enhance the T‐helper type 1 immune response and decrease the PiCV viral loads in pigeons. Significance and Impact of the Study This study proposes that the administration of a probiotic strain, B. velezensis, to pigeons can protect against PiCV infection.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…2018; Santos et al . 2020a). To overcome this situation, an alternative prophylaxis for PiCV and YPDS should be developed.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Probiotic supplementation containing Bacillus velezensis enhances expression of immune regulatory genes against pigeon circovirus in pigeons ( Columba livia )

Tsai

Santos

et al. 2020

J Appl Microbiol

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“…This was verified in the cases of beak and feather disease virus (BFDV), PiCV, and porcine circovirus genotype 2 (PCV2) [11][12][13]. An approved commercial vaccine or prophylaxis against PiCV is not yet available, since there is no laboratory protocol for culturing the virus [14,15]. To date, detection of PiCV infection involves the use of conventional methods including histological observation, electron microscopy, and molecular diagnosis, such as in situ hybridization, nucleic acid-based dot blot hybridization and polymerase chain reaction (PCR) [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity and Protective Activity of Pigeon Circovirus Recombinant Capsid Protein Virus-Like Particles (PiCV rCap-VLPs) in Pigeons (Columba livia) Experimentally Infected with PiCV

et al. 2021

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Pigeon circovirus (PiCV) is the most recurrent virus diagnosed in pigeons and is among the major causative agents of young pigeon disease syndrome (YPDS). Due to the lack of an established laboratory protocol for PiCV cultivation, development of prophylaxis is hampered. Alternatively, virus-like particles (VLPs), which closely resemble native viruses but lack the viral genetic material, can be generated using a wide range of expression systems and are shown to have strong immunogenicity. Therefore, the use of VLPs provides a promising prospect for vaccine development. In this study, transfected human embryonic kidney (HEK-293) cells, a mammalian expression system, were used to express the PiCV capsid protein (Cap), which is a major component of PiCV and believed to contain antibody epitopes, to obtain self-assembled VLPs. The VLPs were observed to have a spherical morphology with diameters ranging from 12 to 26 nm. Subcutaneous immunization of pigeons with 100 µg PiCV rCap-VLPs supplemented with water-in-oil-in-water (W/O/W) adjuvant induced specific antibodies against PiCV. Observations of the cytokine expression and T-cell proliferation levels in spleen samples showed significantly higher T-cell proliferation and IFN- γ expression in pigeons immunized with VLPs compared to the controls (p < 0.05). Experimentally infected pigeons that were vaccinated with VLPs also showed no detectable viral titer. The results of the current study demonstrated the potential use of PiCV rCap-VLPs as an effective vaccine candidate against PiCV.

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Common bacterial, viral, and parasitic diseases in pigeons (Columba livia): A review of diagnostic and treatment strategies

Santos

Tsai

Catulin

et al. 2020

Veterinary Microbiology

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Influence of pigeon interferon alpha (PiIFN-α) on pigeon circovirus (PiCV) replication and cytokine expression in Columba livia

Cited by 16 publications

References 52 publications

Probiotic supplementation containing Bacillus velezensis enhances expression of immune regulatory genes against pigeon circovirus in pigeons ( Columba livia )

Probiotic supplementation containing Bacillus velezensis enhances expression of immune regulatory genes against pigeon circovirus in pigeons ( Columba livia )

Immunogenicity and Protective Activity of Pigeon Circovirus Recombinant Capsid Protein Virus-Like Particles (PiCV rCap-VLPs) in Pigeons (Columba livia) Experimentally Infected with PiCV

Common bacterial, viral, and parasitic diseases in pigeons (Columba livia): A review of diagnostic and treatment strategies

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