Influence of Pickling Process on Allium cepa and Citrus limon Metabolome as Determined via Mass Spectrometry-Based Metabolomics

Farag, Mohamed A.; Tawfike, Ahmed; Donia, Marwa; Ehrlich, Anja; Wessjohann, Ludger A.

doi:10.3390/molecules24050928

Cited by 16 publications

(9 citation statements)

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“…The dereplication approach and multivariate data analysis are used in order to identify compounds in a mixture responsible for the anti-proliferative effects of plant extracts, and provide a better understanding of the mechanisms of action of medicinal plants [ 10 ]. Principle component analysis (PCA) is an unsupervised clustering method requiring no knowledge of the dataset and acts to reduce the dimensionality of multivariate data while preserving most of the variance within [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Activity and Metabolic Profiling of Fifteen Euphorbia Species

et al. 2020

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Euphorbia is a large genus of flowering plants with a great diversity in metabolic pattern. Testing the cytotoxic potential of fifteen Euphorbia species revealed highest activity of E. officinarum L. against human colon adenocarcinoma (CACO2) cell line (IC50 7.2 µM) and of E. lactea Haw. against human hepatoma (HepG2) and human breast adenocarcinoma (MCF-7) cell lines (IC50 5.2 and 5.1 µM, respectively). Additionally, metabolic profiling of the fifteen tested species, using LC-HRMS, for dereplication purposes, led to the annotation of 44 natural compounds. Among the annotated compounds, diterpenoids represent the major class. Dereplication approach and multivariate data analysis are adopted in order to annotate the compounds responsible for the detected cytotoxic activity. Results of Principle component analysis (PCA) come in a great accordance with results of biological testing, which emphasized the cytotoxic properties of E. lactea Haw. A similarity correlation network showed that the two compounds with the molecular formula C16H18O8 and C20H30O10, are responsible for cytotoxic activity against MCF-7 and HepG2 cell lines. Similarly, the compound with molecular formula C18H35NO correlates with cytotoxic activity against CACO2.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic Activity and Metabolic Profiling of Fifteen Euphorbia Species

et al. 2020

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“…In the case of red beet pigments are betalains, which consist of yellow-orange betaxanthins and red-purple betacyanins. Betalain are pigments that are insensitive to pH changes from 3 to 7, which is the reason of its stability during the fermentation process in which pH decreases for beetroot till 3.5 at maximum decrease [ 3 , 19 , 24 ]. In juices the highest color coefficients L* were observed in fresh beetroot juice; during the fermentation process, the lightness independently of the type of bacteria used for fermentation.…”

Section: Resultsmentioning

confidence: 99%

Influence of Fermentation Beetroot Juice Process on the Physico-Chemical Properties of Spray Dried Powder

et al. 2022

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Picking vegetables is, along with salting and drying, one of the oldest ways to preserve food in the world. This is the process of decomposition of simple sugars into lactic acid with the participation of lactic bacteria. The aim of the study was to obtain powders from fermented red beet juice with the highest possible amount of lactic acid bacteria (LAB) and active ingredients. For the analysis, juices were squeezed from the vegetables and two types of fermentation were used: a spontaneous fermentation and a dedicated one. After inoculation, samples were taken for analysis on a daily basis. Extract, pH, total acidity, pigments, and color were measured. In addition, microbiological tests were also carried out. The juices from the fifth day of fermentation was also spray dried, to obtain fermented beetroot powder. Juices from 3–5th day were characterized by a high content of LAB and betanin, had also a low pH, which proves that the lactic fermentation is working properly. The exception was the juice from spontaneous fermentation. According to the observations, the fermentation process did not run properly, and further analysis is needed. The powders were stable; however, results obtained from the pigment content and the LAB content are not satisfactory and require further analysis.

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“…Metabolomics, along with the artificial neural network, was used for sensory evaluation of garlic and garlic products by using 89 quality indicators ( Liu et al, 2019 ) . Farag et al (2019) studied the effect of the pickling process on onion metabolome and reported a decrease in secondary metabolites; thus, pickling using brine negatively affects the health benefits of onion. Functions and application of some metabolites are presented in Table S2 .…”

Section: Survey Methodologymentioning

confidence: 99%

Omics approaches inAlliumresearch: Progress and way ahead

Khandagale

Krishna

Roylawar

et al. 2020

PeerJ

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Background The genus Allium (Family: Amaryllidaceae) is an economically important group of crops cultivated worldwide for their use as a vegetable and spices. Alliums are also well known for their nutraceutical properties. Among alliums, onion, garlic, leek, and chives cultivated worldwide. Despite their substantial economic and medicinal importance, the genome sequence of any of the Allium is not available, probably due to their large genome sizes. Recently evolved omics technologies are highly efficient and robust in elucidating molecular mechanisms of several complex life processes in plants. Omics technologies, such as genomics, transcriptomics, proteomics, metabolomics, metagenomics, etc. have the potential to open new avenues in research and improvement of allium crops where genome sequence information is limited. A significant amount of data has been generated using these technologies for various Allium species; it will help in understanding the key traits in Allium crops such as flowering, bulb development, flavonoid biosynthesis, male sterility and stress tolerance at molecular and metabolite level. This information will ultimately assist us in speeding up the breeding in Allium crops. Method In the present review, major omics approaches, and their progress, as well as potential applications in Allium crops, could be discussed in detail. Results Here, we have discussed the recent progress made in Allium research using omics technologies such as genomics, transcriptomics, micro RNAs, proteomics, metabolomics, and metagenomics. These omics interventions have been used in alliums for marker discovery, the study of the biotic and abiotic stress response, male sterility, organ development, flavonoid and bulb color, micro RNA discovery, and microbiome associated with Allium crops. Further, we also emphasized the integrated use of these omics platforms for a better understanding of the complex molecular mechanisms to speed up the breeding programs for better cultivars. Conclusion All the information and literature provided in the present review throws light on the progress and potential of omics platforms in the research of Allium crops. We also mentioned a few research areas in Allium crops that need to be explored using omics technologies to get more insight. Overall, alliums are an under-studied group of plants, and thus, there is tremendous scope and need for research in Allium species.

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Influence of Pickling Process on Allium cepa and Citrus limon Metabolome as Determined via Mass Spectrometry-Based Metabolomics

Cited by 16 publications

References 50 publications

Cytotoxic Activity and Metabolic Profiling of Fifteen Euphorbia Species

Cytotoxic Activity and Metabolic Profiling of Fifteen Euphorbia Species

Influence of Fermentation Beetroot Juice Process on the Physico-Chemical Properties of Spray Dried Powder

Omics approaches inAlliumresearch: Progress and way ahead

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