Influence of Physiological Media on over-1000 nm NIR Fluorescent DSPE-PEG Micelles for Bio-imaging

Dung, Doan Thi Kim; Umezawa, Masazaku; Kobayashi, Hisanori; Oshima, Atsushi; Ikeda, Kazuno; Okubo, Kyohei; Kamimura, Masao; Soga, Kohei

doi:10.1246/cl.210700

Cited by 4 publications

(11 citation statements)

References 18 publications

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“…At low concentrations, these molecules are distributed as monomers. When the concentration is increased, a dimer distribution is formed owing to the attraction between the positive terminal of one molecule and the BF 4 center of the other [58,66]. IR-1061 also interacts with the hydroxyl ions (OH -) of surrounding water molecules (Fig.…”

Section: Screening Methods For Stability Of Polymer Nanoparticles Usi...mentioning

confidence: 99%

“…As a hydrophobic dye, IR-1061 can be encapsulated in the hydrophobic core of polymeric nanoparticles with the chemical composition described previously [56,60,61,67]; however, the BF 4 counter anion is easily replaced by OHupon the approach of water molecules present in the surrounding hydrophilic environment, resulting in a significant change in absorption wavelength [68]. When encapsulated inside a micelle core, dimer formation is attributed to the Hansen solubility parameter (HSP) affinity between the dye and hydrophobic core [66,67,69]. The matching of HSP affinities results in monomer distribution, whereas mismatching causes dimer formation [58,67].…”

Section: Screening Methods For Stability Of Polymer Nanoparticles Usi...mentioning

confidence: 99%

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Controlling Molecular Dye Encapsulation in the Hydrophobic Core of Core–Shell Nanoparticles for In Vivo Imaging

Umezawa

Ueya²,

Ichihashi

et al. 2023

Biomedical Materials & Devices

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Polymeric nanoparticles with a hydrophobic core are valuable biomedical materials with potential applications in in vivo imaging and drug delivery. These materials are effective at protecting vulnerable molecules, enabling them to serve their functions in hydrophilic physiological environments; however, strategies that allow the chemical composition and molecular weight of polymers to be tuned, forming nanoparticles to control the functional molecules, are lacking. In this article, we review strategies for designing core–shell nanoparticles that enable the effective and stable encapsulation of functional molecules for biomedical applications. IR-1061, which changes its optical properties in response to the microenvironment are useful for in vitro screening of the in vivo stability of polymeric nanoparticles. An in vitro screening test can be performed by dispersing IR-1061-encapsulated polymer nanoparticles in water, saline, buffer solution, aqueous protein solution, etc., and measuring the absorption spectral changes. Through the screening, the effects of the polarity, molecular weight, and the chiral structure of polymers consisting of polymer nanoparticles on their stability have been revealed. Based on the findings presented here, more methodologies for the effective application of various biomolecules and macromolecules with complex high-dimensional structures are expected to be developed.

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Section: Screening Methods For Stability Of Polymer Nanoparticles Usi...mentioning

confidence: 99%

Section: Screening Methods For Stability Of Polymer Nanoparticles Usi...mentioning

confidence: 99%

Controlling Molecular Dye Encapsulation in the Hydrophobic Core of Core–Shell Nanoparticles for In Vivo Imaging

Umezawa

Ueya²,

Ichihashi

et al. 2023

Biomedical Materials & Devices

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“…Structure stability: The probe stability in vitro was assessed using four media: water, saline, PBS, and albumin solution (40 mg/mL) to mimic the biological environments. 16 Samples (20 mg/mL) were dispersed in the four media in 60 min, and thereafter, the absorption, emission, and DLS were measured. The reduction in the R 1 relaxation rates due to the interaction with plasma proteins was assessed using PLP-D [70] and PL-D-P [70].…”

Section: Otn-nir Absorptionmentioning

confidence: 99%

“…The stability of the samples was evaluated using the simple model introduced in our former report. 16 Briefly, the samples were dissolved in four biological media containing multiplex ions and proteins such as water, saline, PBS, and albumin solution to evaluate the effect of Gd-DOTA on the fluorescence properties (Fig. 4).…”

Section: Otn-nir Fluorescence In the Biological Environmentmentioning

confidence: 99%

“…In addition, the absorption shoulder around 850-900 nm is caused by the dimer formation, and the blue shift toward 800 nm indicates an -OH interaction between the dye and the water molecules at the interface. 16,29,30 A noticeable blue shift around 800 nm in the absorption spectrum of the micelle with Gd-DOTA is located at the end of PEG causing derivatization from the highly polarized and hydrophilic structure of DOTAs, those tend to attract water molecules toward its body. Consequently, absorption by the monomer decreases, resulting in the reduction of fluorescence emission compared with the other structures.…”

Section: Effect Of Gd-dota Spatial Distribution On the Probe Characte...mentioning

confidence: 99%

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The effect of Gd-DOTA locations within PLGA-b-PEG micelle encapsulated IR-1061 on bimodal over-1000 nm near-infrared fluorescence and magnetic resonance imaging

et al. 2022

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Enhancing near‐infrared fluorescence intensity and stability of PLGA‐b‐PEG micelles by introducing Gd‐DOTA at the core boundary

Doan,

Umezawa,

Okubo

et al. 2023

J Biomed Mater Res

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Micelles have been extensively used in biomedicine as potential carriers of hydrophobic fluorescent dyes. Their small diameters can potentially enable them to evade recognition by the reticuloendothelial system, resulting in prolonged circulation. Nevertheless, their lack of stability in physiological environments limits the imaging utility of micelles. In particular, when a dye sensitive to water, such as IR‐1061, is encapsulated in the micelle core, the destabilized structure leads to interactions between water and dye, degrading the fluorescence. In this study, we investigated a method to improve micelle stability utilizing the electrical effect of gadolinium (Gd3+) and tetraazacyclododecane tetraacetic acid (DOTA), introduced into the micelles. Three micellar structures, one containing a poly(lactic‐co‐glycolic acid)‐block‐poly(ethylene glycol) (PLGA‐b‐PEG) block copolymer, and two other structures, including PLGA‐b‐PEG with DOTA or Gd‐DOTA introduced at the boundary of PLGA and PEG, were prepared with IR‐1061 in the core. Structures that contained DOTA at the border of the PLGA core and PEG shell exhibited much higher fluorescence intensity than probes without DOTA. With Gd3+ ions at the DOTA center, fluorescence stability was enhanced remarkably in physiological environments. Most interesting is the finding that fluorescence is enhanced with increased Gd‐DOTA concentrations. In conclusion, we found that overall fluorescence and stability are improved by introducing Gd‐DOTA at the boundary of the PLGA core and PEG shell. Improving micelle stability is crucial for further biomedical applications of micellar probes such as bimodal fluorescence and magnetic resonance imaging.

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Influence of Physiological Media on over-1000 nm NIR Fluorescent DSPE-PEG Micelles for Bio-imaging

Cited by 4 publications

References 18 publications

Controlling Molecular Dye Encapsulation in the Hydrophobic Core of Core–Shell Nanoparticles for In Vivo Imaging

Controlling Molecular Dye Encapsulation in the Hydrophobic Core of Core–Shell Nanoparticles for In Vivo Imaging

The effect of Gd-DOTA locations within PLGA-b-PEG micelle encapsulated IR-1061 on bimodal over-1000 nm near-infrared fluorescence and magnetic resonance imaging

Enhancing near‐infrared fluorescence intensity and stability of PLGA‐b‐PEG micelles by introducing Gd‐DOTA at the core boundary

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