Influence of physicochemical and operational parameters on Nitrobacter and Nitrospira communities in an aerobic activated sludge bioreactor

Huang, Zhonghua; Gedalanga, Phillip B.; Asvapathanagul, Pitiporn; Olson, Betty H.

doi:10.1016/j.watres.2010.05.037

Cited by 140 publications

(64 citation statements)

References 33 publications

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“…Since different types of mycolata are selected for at different temperature ranges (5°C to 50°C), foaming may not be temperature restricted but rather triggered by changes in temperature (59). Our study found that temperature alone could not account for the increase in the G. amarae-like cell concentration and is supported by a previous study by Huang et al (29) on nitrite-oxidizing bacteria, which could not separate temperature from the DO effect on population dynamics.…”

Section: Discussionsupporting

confidence: 69%

“…Wastewater samples were collected by using a portable pump (American Sigma, Loveland, CO) from the middle of the aeration tank in 250-ml sterile containers on a weekly basis from DNA extraction. DNA was extracted from 1-ml pure cultures and environmental samples by using a modified bead-beating protocol (67,71), according to methods described previously by Huang et al (29). DNA was extracted from three subsamples of each sample.…”

Section: Methodsmentioning

confidence: 99%

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Interaction of Operational and Physicochemical Factors Leading to Gordonia amarae-Like Foaming in an Incompletely Nitrifying Activated Sludge Plant

Asvapathanagul

Huang

Gedalanga

et al. 2012

Appl Environ Microbiol

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eThe overgrowth of Gordonia amarae-like bacteria in the mixed liquor of an incompletely nitrifying water reclamation plant was inversely correlated with temperature (r ‫؍‬ ؊0.78; P < 0.005) and positively correlated with the solids retention time (SRT) obtained a week prior to sampling (r ‫؍‬ 0.67; P < 0.005). Drops followed by spikes in the food-to-mass ratio (0.18 to 0.52) and biochemical oxygen demand concentrations in primary effluent (94 to 298 mg liter ؊1 ) occurred at the initiation of G. amarae-like bacterial growth. The total bacterial concentration did not increase as concentrations of G. amarae-like cells increased, but total bacterial cell concentrations fluctuated in a manner similar to that of G. amarae-like bacteria in the pseudo-steady state. The ammonium ion removal rate (percent) was inversely related to G. amarae-like cell concentrations during accelerated growth and washout phases. The dissolved oxygen concentration decreased as the G. amarae-like cell concentration decreased. The concentrations of G. amarae-like cells peaked (2.47 ؋ 10 9 cells liter ؊1 ) approximately 1.5 months prior to foaming. Foaming occurred during the late pseudo-steady-state phase, when temperature declines reversed. These findings suggested that temperature changes triggered operational and physicochemical changes favorable to the growth of G. amarae-like bacteria. Fine-scale quantitative PCR (qPCR) monitoring at weekly intervals allowed a better understanding of the factors affecting this organism and indicated that frequent sampling was required to obtain statistical significance with factors changing as the concentrations of this organism increased. Furthermore, the early identification of G. amarae-like cells when they are confined to mixed liquor (10 7 cells liter ؊1 ) allows management strategies to prevent foaming.

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Section: Discussionsupporting

confidence: 69%

Section: Methodsmentioning

confidence: 99%

Interaction of Operational and Physicochemical Factors Leading to Gordonia amarae-Like Foaming in an Incompletely Nitrifying Activated Sludge Plant

Asvapathanagul

Huang

Gedalanga

et al. 2012

Appl Environ Microbiol

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“…Fukushma et al (2013) found that Nitrospira was dominant at high inorganic carbon (IC) condition, while Nitrobacter in low IC. Moreover, Nitrospira was regarded as K-strategists (with high substrate affinities and low maximum activity) for nitrite and oxygen, while Nitrobacter represented r-strategists under limited substrate conditions (Huang et al, 2010), and was able to utilize organic energy sources as a part of facultative autotrophs and anaerobes (Schmidt et al, 2003).…”

Section: Morphologic and Phylogenetic Diversity Of Nobmentioning

confidence: 99%

Detection of nitrifiers and evaluation of partial nitrification for wastewater treatment: A review

et al. 2015

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“…Colliver and Stephenson (2000) reported that most nitrifiers will grow optimally in a temperature range of 25-30°C. A study conducted by Huang et al (2010a) showed that the optimal temperature regime that supported Nitrobacter growth was between 24 and 25°C, whereas higher range of 29-30°C favoured Nitrospira. The activity of the AOB is generally faster than that of NOB because of their different activation energy, which is between 72 and 60 kJ mol -1 for AOB, whereas it is from 43 to 47 kJ mol -1 for NOB (Hulle 2005).…”

Section: Environmental Factors Ph and Temperaturementioning

confidence: 99%

Ecophysiology of nitrifying communities in membrane bioreactors

Awolusi

Kumari

Bux

2014

Int. J. Environ. Sci. Technol.

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Membrane bioreactors (MBRs) are rapidly becoming the technology of choice over conventional activated sludge treatment systems due to their smaller footprint, reduced sludge production, rapid start-up of biological processes, complete removal of suspended solids and better effluent quality. The retention of sufficient amount of slow-growing nitrifiers makes it feasible for the MBRs to achieve strong tolerance against the shock loads with stable and highly efficient nitrogen removal. Various studies have focused on the ecophysiology of nitrifiers in MBRs as well as their distinctive operational parameters as well as their impact on the selection and activity of nitrifying community. Several techniques have been employed over the years to understand the nitrifying community and their interaction within the MBR system, which led to its modification from the initial design. This review focuses on the identification of optimal operational and environmental conditions for efficient nitrification in MBRs. The advantages and limitations of different techniques employed for investigating the nitrifying communities in MBRs are also emphasized.

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Influence of physicochemical and operational parameters on Nitrobacter and Nitrospira communities in an aerobic activated sludge bioreactor

Cited by 140 publications

References 33 publications

Interaction of Operational and Physicochemical Factors Leading to Gordonia amarae-Like Foaming in an Incompletely Nitrifying Activated Sludge Plant

Interaction of Operational and Physicochemical Factors Leading to Gordonia amarae-Like Foaming in an Incompletely Nitrifying Activated Sludge Plant

Detection of nitrifiers and evaluation of partial nitrification for wastewater treatment: A review

Ecophysiology of nitrifying communities in membrane bioreactors

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