Influence of physical activity on tibial bone material properties in laying hens

Rodríguez‐Navarro, Alejandro B.; McCormack, Heather; Fleming, Robert; Álvarez‐Lloret, Pedro; Romero-Pastor, Julia; Domínguez-Gasca, Nazaret; Prozorov, Tanya; Dunn, Ian

doi:10.1016/j.jsb.2017.10.011

Cited by 47 publications

(68 citation statements)

References 45 publications

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“…Infrared spectrometry: The chemical composition of bone tissues (cortical and medullary bone) were analyzed by infrared spectroscopy as previously described [37]. The relative amounts of water, proteins (collagen), lipids, phosphate and carbonate in the bone samples were determined from the peak area of the absorption bands associated with the characteristic molecular groups of each component [38,39].…”

Section: Bone Chemistry and Structurementioning

confidence: 99%

“…X-ray diffraction: Tibiae cortical bone (about 1 × 1 cm) samples cut from the diaphysis were analyzed in transmission mode with a single crystal diffractometer equipped with an area detector (D8 SMART APEX from Bruker) and Mo radiation (50 kV and 30 mA; 0.5 mm collimator). A quantitative estimation of the degree of orientation of apatite crystals (Angular spread; AS) in the cortical bone was determined from the angular breadth of bands displayed in the intensity profile along the Debye-Scherrer ring associated with the 002 reflection of apatite mineral [37].…”

Section: Bone Chemistry and Structurementioning

confidence: 99%

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An eQTL in the cystathionine beta synthase gene is linked to osteoporosis in laying hens

et al. 2020

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Background: Skeletal damage is a challenge for laying hens because the physiological adaptations required for egg laying make them susceptible to osteoporosis. Previously, we showed that genetic factors explain 40% of the variation in end of lay bone quality and we detected a quantitative trait locus (QTL) of large effect on chicken chromosome 1. The aim of this study was to combine data from the commercial founder White Leghorn population and the F2 mapping population to fine-map this QTL and understand its function in terms of gene expression and physiology. Results: Several single nucleotide polymorphisms on chromosome 1 between 104 and 110 Mb (galGal6) had highly significant associations with tibial breaking strength. The alternative genotypes of markers of large effect that flanked the region had tibial breaking strengths of 200.4 vs. 218.1 Newton (P < 0.002) and, in a subsequent founder generation, the higher breaking strength genotype was again associated with higher breaking strength. In a subsequent generation, cortical bone density and volume were increased in individuals with the better bone genotype but with significantly reduced medullary bone quality. The effects on cortical bone density were confirmed in a further generation and was accompanied by increased mineral maturity of the cortical bone as measured by infrared spectrometry and there was evidence of better collagen cross-linking in the cortical bone. Comparing the transcriptome of the tibia from individuals with good or poor bone quality genotypes indicated four differentially-expressed genes at the locus, one gene, cystathionine beta synthase (CBS), having a nine-fold higher expression in the genotype for low bone quality. The mechanism was cis-acting and although there was an amino-acid difference in the CBS protein between the genotypes, there was no difference in the activity of the enzyme. Plasma homocysteine concentration, the substrate of CBS, was higher in the poor bone quality genotype. Conclusions: Validated markers that predict bone strength have been defined for selective breeding and a gene was identified that may suggest alternative ways to improve bone health in addition to genetic selection. The identification of how genetic variants affect different aspects of bone turnover shows potential for translational medicine.

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Section: Bone Chemistry and Structurementioning

confidence: 99%

Section: Bone Chemistry and Structurementioning

confidence: 99%

An eQTL in the cystathionine beta synthase gene is linked to osteoporosis in laying hens

et al. 2020

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“…In the present study, LCD resulted in a significant reduction in laying rate, average egg weight and eggshell quality in caged laying hens. Ca is a critical nutrients and laying hens need to mobilize about 2.2 g of Ca daily for eggshell formation [7]. Long-term Ca deficiency in laying hens will limit the secretion of hormones, leading to reduced or even stopped egg production [17].…”

Section: Discussionmentioning

confidence: 99%

“…Under the long-term and high-yield laying pressure, the Ca circulation of laying hens is in a negative equilibrium state [6]. This state will absorb a large amount of bone Ca for the formation of eggshell, which will reduce the quality of structural bones and eventually lead to CLO [7]. Since production performance and eggshell quality are closely related to bone healthy, CLO has caused enormous economic losses and involved in animal welfare issues [8,9].…”

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confidence: 99%

Total flavonoids of Chinese herb Rhizoma Drynariae alleviates bone loss in caged laying hens

Huang

Tong

et al. 2020

Preprint

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Background: Caged layer osteoporosis (CLO) is a common bone metabolic disease and is threatening the modern poultry industry. The objective of this study was to evaluate effects of total flavonoids of Rhizoma Drynariae (TFRD), a Chinese herbal extract, on bone health, egg quality and production performance in caged laying hens. Totals of 144 Lohmann pink-shell laying hens of 32-wk-old were randomly allocated to 4 groups. The control (CON) group was fed a basal diet, the low calcium diet (LCD) group was fed a low calcium diet, and the TFRD1 and TFRD2 groups were fed LCD supplemented with 0.5 and 2.0 g/kg TFRD, respectively. The experiment lasted 9 wks. Results: Supplying 2.0 g/kg TFRD produced protective effects on calcium deficiency-induced bone loss in caged laying hens, including enhanced femur bone mineral density (P < 0.05), improved bone microarchitecture deterioration, reduced serum levels of alkaline phosphatase and tartrate resistant acid phosphatase activities (both P < 0.05) and osteocalcin content (P < 0.05), down-regulated mRNA expressions of receptor activator of nuclear factor kappa-B ligand (RANKL) (P < 0.01) and up-regulated runt related transcription factor 2 (RUNX2) and osteoprotegerin (OPG) levels (both P < 0.01). Furthermore, compared to LCD group, 2.0 g/kg TFRD treatment had lower malondialdehyd levels (P < 0.01), and higher total antioxidant capacity (P < 0.05) and glutathione peroxidase (P < 0.05) activities, which contributed to the protective effects of bone loss. TFRD supplementation did not affect egg quality while 2.0 g/kg TFRD treatment improved laying rate (P < 0.05) and decreased feed conversion ratio (P < 0.05). Conclusions: These findings suggested that TFRD has beneficial effects on bone health and production performance in caged laying hens, which can be used for the prevention of CLO.

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“…the Ca circulation of laying hens is in a negative equilibrium state [6]. This state will absorb a large amount of bone Ca for the formation of eggshell, which will reduce the quality of structural bones and eventually lead to CLO [7]. Since production performance and eggshell quality are closely related to bone healthy, CLO has caused enormous economic losses and involved in animal welfare issues [8,9].…”

mentioning

confidence: 99%

Total flavonoids of Chinese herb Rhizoma Drynariae alleviates bone loss in caged laying hens

Huang

Tong

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Caged layer osteoporosis (CLO) is a common bone metabolic disease and is threatening the modern poultry industry. The objective of this study was to evaluate effects of total flavonoids of Rhizoma Drynariae (TFRD), a Chinese herbal extract, on bone health, egg quality and production performance in caged laying hens. Totals of 144 Lohmann pink-shell laying hens of 32-wk-old were randomly allocated to 4 groups (CON, LCD, TFRD1 and TFRD2, respectively). The CON group was fed a basal diet, the LCD group was fed a low calcium diet (LCD) and the TFRD1 and TFRD2 groups were fed LCD supplemented with 0.5 and 2.0 g/kg TFRD, respectively.Results: Supplying 2.0 g/kg TFRD produced protective effects on calcium deficiency-induced bone loss in caged laying hens, including reduced serum levels of alkaline phosphatase and tartrate resistant acid phosphatase activities (both P < 0.05) and osteocalcin content (P < 0.05), enhanced femur bone mineral density (P < 0.05), improved bone microarchitecture deterioration, down-regulated mRNA expressions of RANKL (P < 0.01) and up-regulated RUNX2 and OPG levels (both P < 0.01). Furthermore, compared to LCD group, 2.0 g/kg TFRD treatment had lower malondialdehyd levels (P < 0.01), and higher total antioxidant capacity (P < 0.05) and glutathione peroxidase (P < 0.05) activities, which contributed to the protective effects of bone loss. TFRD supplementation did not affect egg quality while 2.0 g/kg TFRD treatment improved laying rate (P < 0.05) and decreased feed conversion ratio (P < 0.05).Conclusions: These findings suggested that TFRD has beneficial effects on bone health and production performance in caged laying hens, which can be used for the prevention of CLO.

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Influence of physical activity on tibial bone material properties in laying hens

Cited by 47 publications

References 45 publications

An eQTL in the cystathionine beta synthase gene is linked to osteoporosis in laying hens

An eQTL in the cystathionine beta synthase gene is linked to osteoporosis in laying hens

Total flavonoids of Chinese herb Rhizoma Drynariae alleviates bone loss in caged laying hens

Total flavonoids of Chinese herb Rhizoma Drynariae alleviates bone loss in caged laying hens

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