Influence of peritoneal fluid from spontaneous and stimulated cycles on sperm motility in vitro

Abstract

Peritoneal fluids (PFs) from spontaneous ( n = 14) and gonadotrophin-stimulated cycles (n = 20) were obtained during diagnostic laparoscopy and gamete intrafallopian transfer (GIFT) procedures, respectively. The effects of these fluids on the linear component of sperm motility and on the percentage of motile spermatozoa were studied in uitra by objective motility assessments and compared to a control medium (B,-Mhkzo).Overall, the two types of PFs were found to have rather similar effects on the motility parameters studied. However, the fluids from hormonally-stimulated cycles sustained motility better (i.e., sperm velocity and percentage of motile sperm) and in a rather constant manner as a function of time (narrower range distributions of the motility measurements). Furthermore, it was observed that under identical experimental conditions motility measurements depended not only on the type of PF used but also on the sperm sample.These results suggest that assisted reproduction procedures in which PF is the medium where the gametes eventually meet and interact, such as direct peritoneal insemination (DIPI) or peritoneal oocyte and sperm transfer (POST), could have different success rates if performed in spontaneous rather than in stimulated cycles. At the same time, our results may help to explain why different pregnancy rates were reported in different studies using D I P or POST.

Impact of Timing on Insemination In Relation to Ovulation on the Cycle Pregnancy Rate of Intrauterine Insemination and Intrauterine Tuboperitoneal Insemination in Unexplained Infertility

“…The effects of these fluids on the percentage of motile spermatozoa were studied by in vitro objective motility assessment and compared to control media. Overall, these fluids from spontaneous and stimulated cycles sustained and increased sperm viability and motility [31]. Peritoneal and follicular fluids from hormonally stimulated cycles sustained sperm motility better than those from spontaneous cycles.…”

“…These studies [30][31][32][33] proved that the fertilizability of sperm and oocytes is upgraded in Douglas pouch by peritoneal and follicular fluids. On the other hand other studies [34,35] evaluated the clinical efficacy of IUI, FSP and DPI by randomized study.…”

The impact of timing of insemination in relation to ovulation on the cycle pregnancy rate of intrauterine insemination and intrauterine tuboperitoneal insemination in unexplained infertility

“…The effects of these fluids on the percentage of motile spermatozoa were studied by in vitro objective motility assessment and compared to control media. Overall, these fluids from spontaneous and stimulated cycles sustained and increased sperm viability and motility (30).…”