Influence of Pathogens, Fish-Related Characteristics, and Environmental Factors on the Development of Skin Ulcerations in Wild Common Dab (Limanda Limanda) From the North Sea

Vercauteren, Maaike; Hoey, Gert Van; Decostere, Annemie; Boyen, Filip; Ampe, Bart; Devriese, Lisa; Chiers, Koen

doi:10.7589/jwd-d-20-00088

Cited by 2 publications

(5 citation statements)

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“…In total, 67 common dabs were caught at various locations in the Belgian part of the North Sea in 2015 ( n = 11), 2016 ( n = 11), and 2017 ( n = 45). More information on the campaigns and capture methods can be found in Vercauteren et al [ 32 ]. Some fish showed an acute or healing skin lesion ( n = 40) or skeletal deformity ( n = 8).…”

Section: Methodsmentioning

confidence: 99%

Explorative study on scale cortisol accumulation in wild caught common dab (Limanda limanda)

Vercauteren

Ampe²,

Devriese

et al. 2022

BMC Vet Res

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Background Flatfish live in a diverse marine ecosystem that is changing due to natural variations and anthropogenic influences. These changes can evoke a stress response mainly resulting in production of the glucocorticoid cortisol, which mediates effects on various levels of biological organization. The finding that cortisol accumulates in fish scales, offering a retrospective view on cortisol production, provides opportunities to use this matrix for chronic stress assessment. The present study is the first to gather information on scale cortisol concentration in wild-caught common dab (Limanda limanda), based on a two-pronged approach using (1) field measurements and (2) a laboratory in vivo-study where wild-caught dab were fed by cortisol-spiked feed during 30 or 90 days to demonstrate the possible accumulation of cortisol in the scales and to evaluate its impact on fish health. Results Based on the field measurements, the average scale cortisol concentration in wild-caught fish was 0.0034 ± 0.0046 µg kg−1 scale (n = 67). This indicates that wild common dab is indeed able to incorporate cortisol in the scales. Based on the experimental data, the cortisol-fed fish showed an increased plasma cortisol concentration (80.16 ± 82.58 µg L−1) compared to the control group (4.54 ± 9.57 µg L−1) after 30 days of cortisol feeding. The increase in plasma cortisol concentration was positively correlated with an increased cortisol concentration in the scale after 30 days of cortisol-spiked feeding. This correlation was, however, no longer observed after 90 days of cortisol-spiked feeding. Interestingly, cortisol concentration of the scales on the pigmented side was significantly higher compared to the non-pigmented side. Some health parameters such as epidermal thickness, body condition and Ichthyobodo sp. parasitic infection showed a correlation with scale cortisol concentration after 30 days. Conclusions We have demonstrated that common dab is able to accumulate cortisol in its scales. This seems to occur proportionally to circulating concentrations of plasma cortisol in fish fed with cortisol supplemented feed after 30 days.

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Section: Methodsmentioning

confidence: 99%

Explorative study on scale cortisol accumulation in wild caught common dab (Limanda limanda)

Vercauteren

Ampe²,

Devriese

et al. 2022

BMC Vet Res

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“…The primary stress response is the activation of the Hypothalamo-Pituitary Interrenal (HPI) axis which culminates in the release of glucocorticoids from inter renal cells located in the head kidney (Lai et al 2021). This leads to the production of corticosteroids mainly cortisol (Kennedy and Jauz 2022; Aidos et al 2020) which then triggers several physiological and metabolic pathways (Vercauteren et al 2021; Sapolsky et al 2000). The action of cortisol is mediated through the glucocorticoid receptor (GR) which is a cytosolic receptor (Cabrera-Busto et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Cortisol has been found to accumulate in fish scales (Vercauteren et al 2021; Carbajal et al 2018; Aerts et al 2015) making it a suitable matrix for a retrospective study of cortisol production and thus for assessment of chronic stress in fish. Several studies have been carried out on scale cortisol of wild caught fish (Vercauteren et al 2021; Roque d’orbcastel et al 2021) but information about scale cortisol of cultured fish remains scanty and poorly documented. Unlike other matrices such as water, serum/plasma, faeces and mucus which reflects short-term circulating cortisol levels, fish scale cortisol represents average levels acquired mainly through passive diffusion over a period of time [30].…”

Section: Introductionmentioning

confidence: 99%

“…This occurs via the Dual-Specificity Phosphatase –1 (DUSP-1) also known as Mitogen activated protein Kinase -1 (MPK -1) (Wang et al 2016). DUSP-1 regulation is the key mechanism of action of glucocorticoids such as Scale cortisol and has been used as a bio-marker for chronic stress in fish (Vercauteren et al 2021; Roque d’orbcastel et al 2021) but information about use of a combination of two or more markers remains scanty and poorly documented. Inhibition of MAPK may be a likely means of inhibiting cell growth (Eanes and Patel 2016).…”

Section: Introductionmentioning

confidence: 99%

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Scale Cortisol Modulation in Cultured Nile Tilapia (Oreochromis niloticus): Deciphering the Intricate Role in Chronic Stress through Mitogen-Activated Protein Kinase Signaling Pathway Regulation

Mwaura,

Angienda,

Wekesa

et al. 2024

Preprint

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Chronic stress poses a significant challenge to aquaculture production, and cortisol and glucose have traditionally served as stress markers. However, recent doubts have emerged about the reliability of scale cortisol as a chronic stress determinant due to its involvement in calcium homeostasis. While cortisol influences gene expression in the stress response cascade, few studies explore its impact on metabolic pathways in cultured Nile tilapia. This study delves into the relationship between cortisol signatures of chronic stress in Nile tilapia and the regulation of the Mitogen Activated Protein Kinase (MAPK) signalling pathway, crucial for cell processes. Juvenile Nile tilapia were subjected to different ammonia concentrations and stocking densities for 70 days, with assessments of growth performance, stress levels, RNA sequencing, and differential gene expression. The results revealed a positive correlation between stressors and blood glucose, plasma cortisol, and scale cortisol concentrations. Fish in water with 0.8 mg/L ammonia exhibited elevated plasma glucose and cortisol, while those in 1.2 mg/L showed increased scale cortisol. Higher stocking densities also correlated with elevated stress markers. Notably, cortisol levels rose with ammonia concentration and stocking density, negatively impacting growth performance. The MAPK signalling pathway, linked to cell processes, showed significant downregulation with increasing ammonia concentrations, suggesting its sensitivity to stress. Taken together, six genes in this pathway were significantly enriched following ammonia treatment (Padj less than or equal to 0.05) i.e. Dual Specific Protein Phosphatase 1 (DUSP1), Nuclear Hormone Receptor 38 (NHR38), Heat Shock Protein 1 (HSP 72 KDa), Myelocytomatosis oncogene homologue (Mych), Growth arrest and DNA damage inducible alpha a (GADD 45aa) and Mitogen Activated Protein Kinase 4 (MAP2K4). This study contributes to unravelling the intricate relationship between chronic stress, cortisol, and the MAPK pathway in aquaculture, providing insights crucial for optimizing fish welfare and production.

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Influence of Pathogens, Fish-Related Characteristics, and Environmental Factors on the Development of Skin Ulcerations in Wild Common Dab (Limanda Limanda) From the North Sea

Cited by 2 publications

References 28 publications

Explorative study on scale cortisol accumulation in wild caught common dab (Limanda limanda)

Explorative study on scale cortisol accumulation in wild caught common dab (Limanda limanda)

Scale Cortisol Modulation in Cultured Nile Tilapia (Oreochromis niloticus): Deciphering the Intricate Role in Chronic Stress through Mitogen-Activated Protein Kinase Signaling Pathway Regulation

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