Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration

Gentile, Piergiorgio; Nandagiri, Vijay Kumar; Pabari, Ritesh M.; Daly, Jacqueline S.; Tonda‐Turo, Chiara; Ciardelli, Gianluca; Ramtoola, Zebunnissa

doi:10.3390/ijms160920492

Cited by 27 publications

(15 citation statements)

References 49 publications

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“…The quantitative evaluation of immunohistochemistry slides was done by using ImageJ software version 1.49 . ImageJ automatically generated the immunohistochemistry index.…”

Section: Methodsmentioning

confidence: 99%

Formulation and in vitro and in vivo evaluation of a new osteoprotegerin–chitosan gel for bone tissue regeneration

Jayash

Hashim

Misran

et al. 2016

J Biomedical Materials Res

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The osteoprotegerin (OPG) system plays a critical role in bone remodelling by regulating osteoclast formation and activity. The study aimed to determine the physicochemical properties and biocompatibility of a newly formulated OPG-chitosan gel. The OPG-chitosan gel was formulated using human OPG protein and water-soluble chitosan. The physicochemical properties were determined using Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Gel morphology was determined using scanning electron microscopy (SEM) and then it was subjected to a protein release assay and biodegradability test. An in vitro cytotoxicity test on normal human periodontal ligament (NHPL) fibroblasts and normal human (NH) osteoblasts was carried out using the AlamarBlue assay. In vivo evaluation in a rabbit model involved creating critical-sized defects in calvarial bone, filling with the OPG-chitosan gel and sacrificing at 12 weeks. In vitro results demonstrated that the 25 kDa OPG-chitosan gel had the highest rate of protein release and achieved 90% degradation in 28 days. At 12 weeks, the defects filled with 25 kDa OPG-chitosan gel showed significant (p < 0.05) new bone formation and the highest expression of osteocalcin and osteopontin compared to controls. Thus, the 25 kDa OPG-chitosan gel could be a promising new biomaterial for tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 398-407, 2017.

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“…The quantitative evaluation of immunohistochemistry slides was done by using ImageJ software version 1.49 . ImageJ automatically generated the immunohistochemistry index.…”

Section: Methodsmentioning

confidence: 99%

Formulation and in vitro and in vivo evaluation of a new osteoprotegerin–chitosan gel for bone tissue regeneration

Jayash

Hashim

Misran

et al. 2016

J Biomedical Materials Res

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“…The EE% could be maximized when the interaction of primary emulsion with external phase was optimal. Peptides are generally subjected to denaturation under various stress conditions, such as sonication and homogenization [33]. Hence, the sonication duration was expected to be critical for the VP5-NP preparation.…”

Section: Discussionmentioning

confidence: 99%

Enhanced and Extended Anti-Hypertensive Effect of VP5 Nanoparticles

Takata

Zhao

et al. 2016

IJMS

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Hypertension has become a significant global public health concern and is also one of the most common risk factors of cardiovascular disease. Recent studies have shown the promising result of peptides inhibiting angiotensin converting enzyme (ACE) in lowering the blood pressure in both animal models and humans. However, the oral bioavailability and continuous antihypertensive effectiveness require further optimization. Novel nanoparticle-based drug delivery systems are helpful to overcome these barriers. Therefore, a poly-(lactic-co-glycolic) acid nanoparticle (PLGANPs) oral delivery system, of the antihypertensive small peptides Val-Leu-Pro-Val-Pro (VLPVP, VP5) model, was developed in this study and its antihypertensive effect was investigated in spontaneously hypertensive rats (SHRs) for the first time. The obtained VP5 nanoparticles (VP5-NPs) showed a small particle size of 223.7 ± 2.3 nm and high entrapment efficiency (EE%) of 87.37% ± 0.92%. Transmission electronic microscopy (TEM) analysis showed that the nanoparticles were spherical and homogeneous. The optimal preparation of VP5-NPs exhibited sustained release of VP5 in vitro and a 96 h long-term antihypertensive effect with enhanced efficacy in vivo. This study illustrated that PLGANPs might be an optimal formulation for oral delivery of antihypertensive small peptides and VP5-NPs might be worthy of further development and use as a potential therapeutic strategy for hypertension in the future.

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“…In another report, human parathyroid hormone‐loaded PLGA NPs have been incorporated in chitosan–gelatin hydrogels for bone regeneration. In vitro PTH release kinetics from PLGA NPs showed a burst release followed by a slow release phase for 3–4 weeks . In another case, 2‐ N , 6 ‐O‐ sulfated chitosan NPs have been incorporated with BMP‐2 peptide.…”

Section: Bone Tementioning

confidence: 99%

“…In vitro PTH release kinetics from PLGA NPs showed a burst release followed by a slow release phase for 3-4 weeks. 62 In another case, 2-N, 6-O-sulfated chitosan NPs have been incorporated with BMP-2 peptide. The developed nanocomplex acted as implant and resulted in enhanced vascularization and also promoted bone augmentation.…”

Section: Bone Tementioning

confidence: 99%

Nanomaterials as potential and versatile platform for next generation tissue engineering applications

et al. 2019

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Tissue engineering (TE) is an emerging field where alternate/artificial tissues or organ substitutes are implanted to mimic the functionality of damaged or injured tissues. Earlier efforts were made to develop natural, synthetic, or semisynthetic materials for skin equivalents to treat burns or skin wounds. Nowadays, many more tissues like bone, cardiac, cartilage, heart, liver, cornea, blood vessels, and so forth are being engineered using 3‐D biomaterial constructs or scaffolds that could deliver active molecules such as peptides or growth factors. Nanomaterials (NMs) due to their unique mechanical, electrical, and optical properties possess significant opportunities in TE applications. Traditional TE scaffolds were based on hydrolytically degradable macroporous materials, whereas current approaches emphasize on controlling cell behaviors and tissue formation by nano‐scale topography that closely mimics the natural extracellular matrix. This review article gives a comprehensive outlook of different organ specific NMs which are being used for diversified TE applications. Varieties of NMs are known to serve as biological alternatives to repair or replace a portion or whole of the nonfunctional or damaged tissue. NMs may promote greater amounts of specific interactions stimulated at the cellular level, ultimately leading to more efficient new tissue formation. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2433–2449, 2019.

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Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration

Cited by 27 publications

References 49 publications

Formulation and in vitro and in vivo evaluation of a new osteoprotegerin–chitosan gel for bone tissue regeneration

Formulation and in vitro and in vivo evaluation of a new osteoprotegerin–chitosan gel for bone tissue regeneration

Enhanced and Extended Anti-Hypertensive Effect of VP5 Nanoparticles

Nanomaterials as potential and versatile platform for next generation tissue engineering applications

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