Using the piezoelectric effect to harvest energy from surrounding vibrations is a promising alternative solution for powering small electronic devices such as wireless sensors and portable devices. A conventional piezoelectric energy harvester (PEH) can only efficiently collect energy within a small range around the resonance frequency. To realize broadband vibration energy harvesting, the idea of multiple-degrees-of-freedom (DOF) PEH to realize multiple resonant frequencies within a certain range has been recently proposed and some preliminary research has validated its feasibility. Therefore, this paper proposed a multi-DOF wideband PEH based on the frequency interval shortening mechanism to realize five resonance frequencies close enough to each other. The PEH consists of five tip masses, two U-shaped cantilever beams and a straight beam, and tuning of the resonance frequencies is realized by specific parameter design. The electrical characteristics of the PEH are analyzed by simulation and experiment, validating that the PEH can effectively expand the operating bandwidth and collect vibration energy in the low frequency. Experimental results show that the PEH has five low-frequency resonant frequencies, which are 13, 15, 18, 21 and 24 Hz; under the action of 0.5 g acceleration, the maximum output power is 52.2, 49.4, 61.3, 39.2 and 32.1 μW, respectively. In view of the difference between the simulation and the experimental results, this paper conducted an error analysis and revealed that the material parameters and parasitic capacitance are important factors that affect the simulation results. Based on the analysis, the simulation is improved for better agreement with experiments.