Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum

Hu, Yanru; Lian, Li; Xia, Jiale; Hu, Shishan; Xu, Weina; Zhu, Jing; Ren, Ang; Shi, Liang; Zhao, Ming

doi:10.1016/j.micres.2019.126348

Cited by 10 publications

(5 citation statements)

References 45 publications

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“…Meanwhile, we identified five differentially expressed transcription factors ( hsf1 , sreA , srrA , snt2 and pacC ), which are also regulated by mystB ‐mediated changes of H3K14 acetylation levels. Among them, disruption of sreA , snt2 and pacC could impair oxidative stress response, sreA negatively impact hydrogen peroxide resistance (Chung et al ., 2020), but snt2 and pacC positively impact hydrogen peroxide resistance (He et al ., 2018; Hu et al ., 2020). SrrA was a constitutive nuclear protein, not only could cooperate with hsf1 to induce heat shock genes in response specifically to oxidative stress (Raitt et al ., 2000) but also combine with Yap1 to respond to peroxides (Mulford and Fassler, 2011).…”

Section: Discussionmentioning

confidence: 99%

Histone acetyltransferases MystA and MystB contribute to morphogenesis and aflatoxin biosynthesis by regulating acetylation in fungus Aspergillus flavus

Chen

Lan

et al. 2021

Environmental Microbiology

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Myst family is highly conserved histone acetyltransferases in eukaryotic cells and is known to play crucial roles in various cellular processes; however, acetylation catalysed by acetyltransferases is unclear in filamentous fungi. Here, we identified two classical nonessential Myst enzymes and analysed their functions in Aspergillus flavus, which generates aflatoxin B1, one of the most carcinogenic secondary metabolites. MystA and MystB located in nuclei and cytoplasm, and mystA could acetylate H4K16ac, while mystB acetylates H3K14ac, H3K18ac and H3K23ac. Deletion mystA resulted in decreased conidiation, increased sclerotia formation and aflatoxin production. Deletion of mystB leads to significant defects in conidiation, sclerotia formation and aflatoxin production. Additionally, double-knockout mutant (ΔmystA/mystB) display a stronger and similar defect to ΔmystB mutant, indicating that mystB plays a major role in regulating development and aflatoxin production. Both mystA and mystB play important role in crop colonization. Moreover, catalytic domain MOZ and the catalytic site E199/E243 were important for the acetyltransferase function of Myst. Notably, chromatin immunoprecipitation results indicated that mystB participated in oxidative detoxification by regulating the acetylation level of H3K14, and further regulated nsdD to affect sclerotia formation and aflatoxin production. This study provides new evidences to discover the biological functions of histone acetyltransferase in A. flavus.

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Section: Discussionmentioning

confidence: 99%

Histone acetyltransferases MystA and MystB contribute to morphogenesis and aflatoxin biosynthesis by regulating acetylation in fungus Aspergillus flavus

Chen

Lan

et al. 2021

Environmental Microbiology

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“…In addition, the pH-responsive transcription factor Rim101/PacC family govern pH-dependent morphological transition and virulence in many fungal pathogens (237)(238)(239)(240)(241). Interestingly, the homologous gene in Ganoderma lucidum, GlPacC, was developmentally regulated during fruiting body formation, and the GlPacC-silenced strain was defective in primordium formation (242); its cell wall was 25 to 30% thinner and contained ;20% less b-1,3-glucan than that of the wild-type strain (243).…”

Section: Genetic Bases Of Fruiting Body Developmentmentioning

confidence: 99%

Evolutionary Morphogenesis of Sexual Fruiting Bodies in Basidiomycota: Toward a New Evo-Devo Synthesis

Virágh

Merényi

Csernetics

et al. 2022

Microbiol Mol Biol Rev

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“…Hu et al . (2020) found that PacC/Rim101 could activate the expression of ENA1 , the gene encoding Na + pump ATPase, in response to Na + stress, and the deletion of PacC resulted in the inability to activate ENA1 . In addition, the ionic stress response gene encoding Na + /K + ‐ATPase is also a downstream target of PacC , and its expression is directly regulated by PacC , thereby regulating the balance of intracellular Na + and K + (Huang et al ., 2015).…”

Section: Discussionmentioning

confidence: 99%

The pH signalling transcription factor PacC modulate growth, development, stress response and pathogenicity of Trichothecium roseum

Wang

Han

Gong

et al. 2022

Environmental Microbiology

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pH is one of the important environmental factors that affect the growth, development and pathogenicity of postharvest pathogen. The transcription factor PacC dominates the pH signal pathway. PacC in Trichothecium roseum showed three typical conserved zinc finger domains and closest homology to Fusarium graminearum. T. roseum increased the environmental pH both in vitro and in vivo. Expression patterns of TrpacC under different pH showed that at increasing pH from 3 to 5, the wild-type (WT) strain induced the expression of TrPacC in parallel to increased fungal growth; however, TrPacC expression decline at higer pH than 5, while fungal growth continued to increase. Development of a ΔTrPacC mutant down-regulated the expression of TrbrlA, TrabaA and TrwetA, reduced sporulation and delayed spore germination, resulting in smaller spores and sparse hyphae. ΔTrPacC mutant was sensitive to ionic stress, oxidative stress and cell wall integrity stress compared to the WT strain, especially the ionic stress. In addition, ΔTrPacC mutant showed reduced pathogenicity to muskmelon and tomato fruits. Taken together, T. roseum is an alkalinizing fungus, and the acidic environment could induce TrPacC expression. TrPacC positively regulates fungal growth and development as well as pathogenicity showing effect on fungal response to different stresses.

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Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum

Cited by 10 publications

References 45 publications

Histone acetyltransferases MystA and MystB contribute to morphogenesis and aflatoxin biosynthesis by regulating acetylation in fungus Aspergillus flavus

Histone acetyltransferases MystA and MystB contribute to morphogenesis and aflatoxin biosynthesis by regulating acetylation in fungus Aspergillus flavus

Evolutionary Morphogenesis of Sexual Fruiting Bodies in Basidiomycota: Toward a New Evo-Devo Synthesis

The pH signalling transcription factor PacC modulate growth, development, stress response and pathogenicity of Trichothecium roseum

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