Influence of oxygen level on oxidative stress response of Bacillus sp. F26 to menadione

Yan, Guoliang; Hua, Zhaozhe; Liu, Dengru; Du, Guocheng; Chen, Jian

doi:10.1016/j.procbio.2005.09.011

Cited by 8 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various reagents that induce oxidative stress during submerged fermentation affect the growth rate and biomass accumulation of microorganisms and modulate their production of SMs. ,,,, Considering the antioxidant and free radical scavenging activities of antrodin C, we hypothesized that supplementation of A. cinnamomea fermentations with oxidative stress inducers may provoke this fungus to upregulate the biosynthesis of antrodin C. To evaluate this hypothesis, we applied diethyl phthalate (a toxic compound that elevates intracellular ROS levels), menadione (a producer of semiquinone and superoxide radicals), and H 2 O 2 (a microbicidal oxidizing agent) − during A.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, SM production may be elicited by environmental stimuli, such as biotic and abiotic stressors. One of the most common physiological stress conditions in the habitat of filamentous fungi is oxidative stress that leads to the formation of harmful reactive oxygen species (ROS) in the cells. , ROS, including hydrogen peroxide (H 2 O 2 ), hydroxyl radical (HO · ), and superoxide anions (O 2– ), cause dose-dependent damage to most biomolecules such as DNA, proteins, and lipids. , Microbial cells respond to ROS by initiating scavenging mechanisms, including enzymatic or nonenzymatic systems. , Growing evidence also suggests that ROS play vital physiological roles in the regulation of morphogenesis, differentiation, and secondary metabolism in filamentous fungi. , Thus, modulation of the oxidative stress response of Aspergillus flavus by cinnamaldehyde effectively inhibits radial growth, spore production, and mycelium formation . Methyl jasmonate induced the biosynthesis of ganoderic acids by increasing the intracellular ROS concentration of Ganoderma lucidum .…”

Section: Introductionmentioning

confidence: 99%

“…One of the most common physiological stress conditions in the habitat of filamentous fungi is oxidative stress that leads to the formation of harmful reactive oxygen species (ROS) in the cells. 3,4 ROS, including hydrogen peroxide (H 2 O 2 ), hydroxyl radical (HO • ), and superoxide anions (O 2− ), cause dose-dependent damage to most biomolecules such as DNA, proteins, and lipids. 3,5 Microbial cells respond to ROS by initiating scavenging mechanisms, including enzymatic or nonenzymatic systems.…”

Section: Introductionmentioning

confidence: 99%

“…3,4 ROS, including hydrogen peroxide (H 2 O 2 ), hydroxyl radical (HO • ), and superoxide anions (O 2− ), cause dose-dependent damage to most biomolecules such as DNA, proteins, and lipids. 3,5 Microbial cells respond to ROS by initiating scavenging mechanisms, including enzymatic or nonenzymatic systems. 6,7 Growing evidence also suggests that ROS play vital physiological roles in the regulation of morphogenesis, differentiation, and secondary metabolism in filamentous fungi.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Oxidative Stress Induction Is a Rational Strategy to Enhance the Productivity of Antrodia cinnamomea Fermentations for the Antioxidant Secondary Metabolite Antrodin C

Huang

Chen

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

Antioxidant metabolites contribute to alleviating oxidative stress caused by reactive oxygen species (ROS) in microorganisms. We utilized oxidative stressors such as hydrogen peroxide supplementation to increase the yield of the bioactive secondary metabolite antioxidant antrodin C in submerged fermentations of the medicinal mushroom Antrodia cinnamomea. Changes in the superoxide dismutase and catalase activities of the cells indicate that ROS are critical to promote antrodin C biosynthesis, while the ROS production inhibitor diphenyleneiodonium cancels the productivity-enhancing effects of H 2 O 2 . Transcriptomic analysis suggests that key enzymes in the mitochondrial electron transport chain are repressed during oxidative stress, leading to ROS accumulation and triggering the biosynthesis of antioxidants such as antrodin C. Accordingly, rotenone, an inhibitor of the electron transport chain complex I, mimics the antrodin C productivity-enhancing effects of H 2 O 2 . Delineating the steps connecting oxidative stress with increased antrodin C biosynthesis will facilitate the fine-tuning of strategies for rational fermentation process improvement.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Oxidative Stress Induction Is a Rational Strategy to Enhance the Productivity of Antrodia cinnamomea Fermentations for the Antioxidant Secondary Metabolite Antrodin C

Huang

Chen

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Therefore, histidine-tagged affinity chromatography is most frequently used for the purification of recombinant proteins. Immobilized metal affinity chromatography (IMAC) is mostly used as an effective method for the capture of histidine-tagged proteins from complex protein mixtures [9][10].…”

Section: Introductionmentioning

confidence: 99%

Breakthrough Model of Recombinant Human-Like Collagen in Immobilized Metal Affinity Chromatography

Wang

Fan

Luo

2008

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The adsorption of recombinant human-like collagen by metal chelate media was investigated in a batch reactor and in a fixed-bed column. The adsorption equilibrium and kinetics had been studied by batch adsorption experiments. Equilibrium parameters and protein diffusivities were estimated by matching the models with the experimental data. Using the parameters of equilibrium and kinetics, various models, such as axial diffusion model, linear driving force model, and constant pattern model, were used to simulate the breakthrough curves on the columns. As a result, the most suitable isotherm was the Langmuir-Freundlich model, and the ionic strength had no effect on the adsorption capacity of chelate media. In addition, the pore diffusion model fitted very well to the kinetic data. The pore diffusivities decreased with increasing the initial protein concentration, however had little change with the ionic strength. The results also indicated that the models predict breakthrough curves reasonably well to the experimental data, especially at low initial protein concentration (0.3 mg ml(-1)) and low flow rate (34 cm h(-1)). By the results, we optimized the experimental conditions of a chromatographic process using immobilized metal affinity chromatography to purify recombinant human-like collagen.

show abstract

Enhanced poly(γ‐glutamic acid) production by H₂O₂‐induced reactive oxygen species in the fermentation of Bacillus subtilis NX‐2

Tang

Zhang

et al. 2015

Biotech and App Biochem

View full text Add to dashboard Cite

Effects of reactive oxygen species (ROS) on cell growth and poly(γ-glutamic acid) (γ-PGA) synthesis were studied by adding hydrogen peroxide to a medium of Bacillus subtilis NX-2. After optimizing the addition concentration and time of H O , a maximum concentration of 33.9 g/L γ-PGA was obtained by adding 100 µM H O to the medium after 24 H. This concentration was 20.6% higher than that of the control. The addition of diphenyleneiodonium chloride (ROS inhibitor) can interdict the effect of H O -induced ROS. Transcriptional levels of the cofactors and relevant genes were also determined under ROS stress to illustrate the possible metabolic mechanism contributing to the improve γ-PGA production. The transcriptional levels of genes belonging to the tricarboxylic acid cycle and electron transfer chain system were significantly increased by ROS, which decreased the NADH/NAD ratio and increased the ATP levels, thereby providing more reducing power and energy for γ-PGA biosynthesis. The enhanced γ-PGA synthetic genes also directly promoted the formation of γ-PGA. This study was the first to use the ROS control strategy for γ-PGA fermentation and provided valuable information on the possible mechanism by which ROS regulated γ-PGA biosynthesis in B. subtilis NX-2.

show abstract

Influence of oxygen level on oxidative stress response of Bacillus sp. F26 to menadione

Cited by 8 publications

References 23 publications

Oxidative Stress Induction Is a Rational Strategy to Enhance the Productivity of Antrodia cinnamomea Fermentations for the Antioxidant Secondary Metabolite Antrodin C

Oxidative Stress Induction Is a Rational Strategy to Enhance the Productivity of Antrodia cinnamomea Fermentations for the Antioxidant Secondary Metabolite Antrodin C

Breakthrough Model of Recombinant Human-Like Collagen in Immobilized Metal Affinity Chromatography

Enhanced poly(γ‐glutamic acid) production by H₂O₂‐induced reactive oxygen species in the fermentation of Bacillus subtilis NX‐2

Contact Info

Product

Resources

About

Influence of oxygen level on oxidative stress response of Bacillus sp. F26 to menadione

Cited by 8 publications

References 23 publications

Oxidative Stress Induction Is a Rational Strategy to Enhance the Productivity of Antrodia cinnamomea Fermentations for the Antioxidant Secondary Metabolite Antrodin C

Oxidative Stress Induction Is a Rational Strategy to Enhance the Productivity of Antrodia cinnamomea Fermentations for the Antioxidant Secondary Metabolite Antrodin C

Breakthrough Model of Recombinant Human-Like Collagen in Immobilized Metal Affinity Chromatography

Enhanced poly(γ‐glutamic acid) production by H2O2‐induced reactive oxygen species in the fermentation of Bacillus subtilis NX‐2

Contact Info

Product

Resources

About

Enhanced poly(γ‐glutamic acid) production by H₂O₂‐induced reactive oxygen species in the fermentation of Bacillus subtilis NX‐2