Influence of oxygen availability on the activities of ammonia‐oxidizing archaea

Qin, Wei; Meinhardt, Kelley A.; Moffett, James W.; Devol, Allan H.; Armbrust, E. Virginia; Ingalls, Anitra E.; Stahl, David A.

doi:10.1111/1758-2229.12525

Cited by 102 publications

(115 citation statements)

References 56 publications

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“…We do not have a direct explanation for this effect, but it is possible that hydrogen peroxide has a negative effect on competitors of Thaumarchaea at these sites. This is supported by recent studies reported by Qin et al (), demonstrating that some AOA are resistant to extremely high peroxide concentrations (> 1 μ mol L −1 ). Thus, our data indicate that direct irradiation exerts a more fundamental control on ammonia oxidation, at least in open ocean regimes, and that H 2 O 2 would have a modest effect on ammonia oxidation within the mixed layer and a negligible effect below.…”

Section: Discussionsupporting

confidence: 87%

Relative impacts of light, temperature, and reactive oxygen on thaumarchaeal ammonia oxidation in the North Pacific Ocean

Horak

Qin

Bertagnolli

et al. 2017

Limnology & Oceanography

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Thaumarchaeota are implicated as the major ammonia oxidizers in the ocean. However, the influence of various abiotic factors in determining their distribution and activity in the upper ocean remain largely unclear. Here, we examined the influence of light, hydrogen peroxide (H2O2), and temperature on ammonia oxidation rates for communities dominated by Thaumarchaeota at the nitrite maximum across two North Pacific transects. In situ ammonia oxidation was almost exclusively driven by Thaumarchaeota, as inferred from ammonia monooxygenase subunit A (amoA) genes, amoA transcripts, and inhibitor studies. A major shift in population structure near the eastern North Pacific Subtropical Front was revealed by sequence variation of amoA genes, showing different Thaumarchaeota community structure in oligotrophic gyre and temperate regions. While the most dominant OTUs were closely related, we found significant differences in physiological responses to light and temperature of incubation. At four stations in different biogeochemical regimes, the impact of sunlight intensity and temperature on activity was evaluated using 15 NH4+‐spiked whole seawater collected from the nitrite maximum and incubated at different depths on a free floating in situ array. Ammonia oxidation was usually completely inhibited by PAR at the surface and 21–45% inhibited at 1% surface PAR, whereas a temperature effect on ammonia oxidation was observed at only two of four stations. While inhibition due to H2O2 cannot be ruled out in surface waters, our findings show that below the mixed layer, photoinhibition, and not H2O2 toxicity, had a greater influence on ammonia oxidation.

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Section: Discussionsupporting

confidence: 87%

Relative impacts of light, temperature, and reactive oxygen on thaumarchaeal ammonia oxidation in the North Pacific Ocean

Horak

Qin

Bertagnolli

et al. 2017

Limnology & Oceanography

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“…Bakken, 1999;Shaw et al, 2006), due to differences in available NH + 4 and oxygen concentrations that affect enzymatic reactions. N 2 O yields from AOA cultures are below or in the lower range of those observed for AOB, that is, 0.04%-0.3%, and NH + 4 and oxygen concentration have no or little effect on yield Hink, Lycus, et al, 2017;Kim et al, 2012;Löscher et al, 2012;Santoro et al, 2011;Stieglmeier, Mooshammer, et al, 2014;Qin et al, 2017). An alternative explanation is redox balancing, in which electrons generated by hydroxylamine dehydrogenase are shuttled to denitrification enzymes when they exceed the capacity of terminal oxidases (Hink, Lycus, et al, 2017).…”

Section: Dis Tin C Tion Of Archae Al and Bac Terial N 2 O Produc Tionmentioning

confidence: 95%

Nitrous oxide production by ammonia oxidizers: Physiological diversity, niche differentiation and potential mitigation strategies

Prosser

Hink

Gubry‐Rangin

et al. 2019

Global Change Biology

278

157

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Oxidation of ammonia to nitrite by bacteria and archaea is responsible for global emissions of nitrous oxide directly and indirectly through provision of nitrite and, after further oxidation, nitrate to denitrifiers. Their contributions to increasing N 2 O emissions are greatest in terrestrial environments, due to the dramatic and continuing increases in use of ammonia-based fertilizers, which have been driven by requirement for increased food production, but which also provide a source of energy for ammonia oxidizers (AO), leading to an imbalance in the terrestrial nitrogen cycle.Direct N 2 O production by AO results from several metabolic processes, sometimes combined with abiotic reactions. Physiological characteristics, including mechanisms for N 2 O production, vary within and between ammonia-oxidizing archaea (AOA) and bacteria (AOB) and comammox bacteria and N 2 O yield of AOB is higher than in the other two groups. There is also strong evidence for niche differentiation between AOA and AOB with respect to environmental conditions in natural and engineered environments. In particular, AOA are favored by low soil pH and AOA and AOB are, respectively, favored by low rates of ammonium supply, equivalent to application of slow-release fertilizer, or high rates of supply, equivalent to addition of high concentrations of inorganic ammonium or urea. These differences between AOA and AOB provide the potential for better fertilization strategies that could both increase fertilizer use efficiency and reduce N 2 O emissions from agricultural soils. This article reviews research on the biochemistry, physiology and ecology of AO and discusses the consequences for AO communities subjected to different agricultural practices and the ways in which this knowledge, coupled with improved methods for characterizing communities, might lead to improved fertilizer use efficiency and mitigation of N 2 O emissions.

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“…The yield of N2O (molar ratio of N2O production to ammonium oxidation) increases with decreasing oxygen (Goreau et al, 1980). Culture (Qin et al, 2017) and field studies (Bristow et al, 2016;Peng et al, 2016) have shown high affinity of oxygen (< 5 μmol L -1 ) during ammonium oxidation. The main sources of ammonium in the Chesapeake Bay include remineralization of organic matter in the oxygenated water column and sediments (Kemp et al, 1990) and atmospheric deposition (Larsen et al, 15 2001).…”

Section: The Sensitivities To Increasing [O2] Of No2mentioning

confidence: 99%

Nitrogen and oxygen availabilities control water column nitrous oxide production during seasonal anoxia in the Chesapeake Bay

Ji¹,

Frey²,

Sun³

et al. 2018

Preprint

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Abstract. Nitrous oxide (N2O) is a greenhouse gas and an ozone depletion agent. One of the major uncertainties in the global N2O budget is the contribution of the coastal region, including estuaries, which can be sites of intense N2O efflux.Incubation experiments with nitrogen stable isotope tracer ( 15 N) enabled the investigation of the environmental controls of 10 N2O production in the water column of Chesapeake Bay, the largest estuary in North America. The highest potential rates of N2O production (7.5±1.2 nmol-N L -1 hr -1 ) were detected during summer anoxia, during which oxidized nitrogen species (nitrate and nitrite) were absent from the water column. At the top of the anoxic layer, N2O production from denitrification was stimulated by addition of nitrate and nitrite. The relative contribution of nitrate and nitrite to N2O production was positively correlated with the ratio of nitrate to nitrite concentrations. Increased oxygen availability, up to 7 µM oxygen 15 inhibited both N2O production and the reduction of nitrate to nitrite. Therefore, reducing the nitrogen input into the Chesapeake Bay has two potential impacts on the N2O efflux: In the short-term, N2O emission will be mitigated due to nitrogen deficiency. In the long-run, eutrophication will be alleviated and subsequent re-oxygenation of the bay will further inhibit N2O production.

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Influence of oxygen availability on the activities of ammonia‐oxidizing archaea

Cited by 102 publications

References 56 publications

Relative impacts of light, temperature, and reactive oxygen on thaumarchaeal ammonia oxidation in the North Pacific Ocean

Relative impacts of light, temperature, and reactive oxygen on thaumarchaeal ammonia oxidation in the North Pacific Ocean

Nitrous oxide production by ammonia oxidizers: Physiological diversity, niche differentiation and potential mitigation strategies

Nitrogen and oxygen availabilities control water column nitrous oxide production during seasonal anoxia in the Chesapeake Bay

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