Influence of over-expression of cytosolic aspartate aminotransferase on amino acid metabolism and defence responses against Botrytis cinerea infection in Arabidopsis thaliana

Brauc, Sigrid; Vooght, E De; Claeys, Martine; Höfte, Monica; Angenon, Geert

doi:10.1016/j.jplph.2011.05.012

Cited by 57 publications

(45 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In rice, normal root elongation requires arginine produced by OsASS and OsASL (Xia et al, 2014). Aspartate transaminase (AspAT) is an important enzyme in amino acid metabolism which catalyzes the interconversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate (Brauc et al, 2011). Eleven AspAT genes were found and expressed in all three stages of fruit development (Table S10).…”

Section: Resultsmentioning

confidence: 99%

“…Eleven AspAT genes were found and expressed in all three stages of fruit development (Table S10). In A. thaliana , overexpression the cytosolic AtAspAT influences amino acid metabolism and defense responses against Botrytis cinerea infection (Brauc et al, 2011). Those genes ( GAD, GDH, GOGAT, ASS, ASL, AspAT ) involved in glutamine, arginine, and aspartate metabolism may take part in stress response or development in kiwifruit.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis)

et al. 2016

View full text Add to dashboard Cite

Genomic and transcriptomic data on kiwifruit (Actinidia chinensis) in public databases are very limited despite its nutritional and economic value. Previously, we have constructed and sequenced nine fruit RNA-Seq libraries of A. chinensis “Hongyang” at immature, mature, and postharvest ripening stages of fruit development, and generated over 66.2 million paired-end and 24.4 million single-end reads. From this dataset, here we have identified 7051 long noncoding RNAs (lncRNAs), 29,327 alternative splicing (AS) events and 2980 novel protein-coding genes that were not annotated in the draft genome of “Hongyang.” AS events were demonstrated in genes involved in the synthesis of nutritional metabolites in fruit, such as ascorbic acids, carotenoids, anthocyanins, and chlorophylls, and also in genes in the ethylene signaling pathway, which plays an indispensable role in fruit ripening. Additionally, transcriptome profiles and the contents of sugars, organic and main amino acids were compared between immature, mature, and postharvest ripening stages in kiwifruits. A total of 5931 differentially expressed genes were identified, including those associated with the metabolism of sugar, organic acid, and main amino acids. The data generated in this study provide a foundation for further studies of fruit development and ripening in kiwifruit, and identify candidate genes and regulatory elements that could serve as targets for improving important agronomic traits through marker assisted breeding and biotechnology.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis)

et al. 2016

View full text Add to dashboard Cite

show abstract

“…On the other hand, maintenance of redundancy can confer robustness against mutations (De Smet and Van de Peer, 2012) and/or a selective advantage in increasing the abundance of encoded proteins (Bekaert et al , 2011). As GS1 is an essential enzyme of primary N metabolism, linked to central carbon metabolism via the GS/GOGAT cycle that might also play a role in the adaptation of plant to nutrient deficiency and pathogen attack (Brauc et al , 2011; Seifi et al , 2013), maintenance of multicopy of GLN1 could confer robustness against mutations. Nevertheless, GLN1 expression profiles have not been exhaustively investigated and there might be particular environmental or developmental conditions allowing the differentiation of expression profiles between homeologous and/or paralogous genes.…”

Section: Discussionmentioning

confidence: 99%

Sixteen cytosolic glutamine synthetase genes identified in the Brassica napus L. genome are differentially regulated depending on nitrogen regimes and leaf senescence

Orsel¹,

Moison²,

Clouet³

et al. 2014

Journal of Experimental Botany

View full text Add to dashboard Cite

show abstract

“…Similar experiments were performed for the complementation of egy1 mutant to ensure the function of ethylene-dependent gravitropism-deficient and yellowgreen 1 in regulation of leaf development and senescence in A. thaliana. [27] Several other investigators also performed the same experiment to confirm the role of a respective genes such as TLP 18.3 gene, [12] ASP2 gene, [28] and CYP724A1 gene. [29] We have previously established physiological parameters for the screening of leaf senescence that based on the determination of chlorophyll content, ion leakage, and MDA content at different leaf developmental stages.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Ansari¹

2017

IJGP

View full text Add to dashboard Cite

Aim: Gamma-aminobutyric acid (GABA) is the chief inhibitory neurotransmitter in the mammalian central nervous system, and its important role is reducing the neuronal excitability throughout the nervous system. The GABA transaminase (GABA-T) enzyme catalyzes the transamination of GABA to form succinic semialdehyde using pyruvate as amino acid acceptor in GABA shunt pathway. The Arabidopsis thaliana GABA-T gene (pollenpistil incompatibility 2 [POP2]; At3g22200) belongs to Class III pyridoxal-phosphate-dependent aminotransferase family. The full-length cDNA of the gene encodes a protein of 513 amino acids residues and located at chromosome 3. It could be demonstrated that the GABA-T is involved in the regulation of leaf senescence in A. thaliana. Materials and Methods: To ensure the role of GABA-T gene in leaf senescence, we have performed functional complementation using transformation of pPZP200GB-GABA-T construct into pop2-3 T-DNA insertion mutant (GABA-T knockout mutant) of A. thaliana (GABA-T complement plant) that the mutant plant reverts back to wild-type plant. We have measured and compared the physiological characteristics associated with senescence (chlorophyll content, ion leakage and lipid peroxidation) and quantified the GABA shunt components (GABA content, GABA-T, and glutamate decarboxylase activity) of the wild-type, pop2-3 mutant, and GABA-T complement plant of A. thaliana. Results: The result showed the low chlorophyll content, increased ion leakage, and higher level of lipid peroxidation in pop2-3 mutant than wild-type plant after 30 days in normal growth condition. GABA-T pop2-3 mutants act as signals that mediate early leaf senescence. GABA-T complement plant shared similar characteristics of senescence parameters with wild-type plants. Conclusion: These data concluded that GABA-T is involved in the regulation of leaf senescence in A. thaliana.

show abstract

Influence of over-expression of cytosolic aspartate aminotransferase on amino acid metabolism and defence responses against Botrytis cinerea infection in Arabidopsis thaliana

Cited by 57 publications

References 48 publications

Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis)

Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis)

Sixteen cytosolic glutamine synthetase genes identified in the Brassica napus L. genome are differentially regulated depending on nitrogen regimes and leaf senescence

Untitled

Contact Info

Product

Resources

About