Influence of osteocalcin and collagen I on the mechanical and biological properties of Biocement D

Knepper-Nicolai, Birgit; Reinstorf, Antje; Hofinger, I.; Flade, Katharina; Wenz, R.; Pompe, W.

doi:10.1016/s1389-0344(02)00036-9

Cited by 70 publications

(59 citation statements)

References 13 publications

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“…These bone defects might be caused by trauma or other genesis, e.g., a benign tumor, from surgery or congenital. Calcium phosphate bone substitutes impregnated with, for instance, bone marrow aspirate (BMA) [81], autologous platelet-rich plasma (PRP) [82], osteocalcin [83] or mesenchymal stem cells [84] were already shown to significantly improve the skeletal repair abilities of the otherwise pristine materials. Therefore, these granules are expected to perform better in vivo when they are first impregnated with PRP or BMA and then used.…”

Section: Resultsmentioning

confidence: 99%

Preparation of porous apatite granules from calcium phosphate cement

Taş

2007

J Mater Sci: Mater Med

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A versatile method for preparing spherical, micro-and macroporous (micro: 2-10 and macro: 150-550 lm pores), carbonated apatitic calcium phosphate (Ap-CaP) granules (2-4 mm in size) was developed by using NaCl crystals as the porogen. The entire granule production was performed between 21 and 37°C. A CaP cement powder, comprising a-Ca 3 (PO 4 ) 2 (61 wt.%), CaH-PO 4 (26%), CaCO 3 (10%) and precipitated hydroxyapatite, Ca 10 (PO 4 ) 6 (OH) 2 (3%), was dry mixed with NaCl crystals varying in size from 420 lm to 1 mm. Cement powder (35 wt.%) and NaCl (65 wt.%) mixture was kneaded with an ethanol-Na 2 HPO 4 initiator solution, and the formed dough was immediately agitated on an automatic sieve shaker for a few minutes to produce the spherical granules. Embedded NaCl crystals were then leached out of the granules by soaking them in deionized water. CaP granules were micro-and macroporous with a total porosity of 50% or more. Granules were composed of carbonated, poorly crystallized, apatitic CaP phase. These were the first spherical and porous CaP granules ever produced from a self-setting calcium phosphate cement. The granules reached their final handling strength at the ambient temperature through the cement setting reaction, without having a need for sintering.

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Section: Resultsmentioning

confidence: 99%

Preparation of porous apatite granules from calcium phosphate cement

Taş

2007

J Mater Sci: Mater Med

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“…[28][29][30]35 For example, Hempel et al described that the toxic effects of unmodified CaP cement are due to physicochemically unstable discs and released phosphate and protons into the culture medium, resulting in a decrease in pH and an uptake of calcium by the specimens. 30 The decrease in pH, which was reported to affect cell attachment and behavior of the osteoblast-like cells in vitro, was also observed in our experiments with the unmodified and preincubated samples.…”

Section: Discussionmentioning

confidence: 99%

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

et al. 2007

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In this study, the cytocompatibility and early osteogenic characteristics of rat bone marrow cells (RBMCs) on injectable calcium phosphate (CaP) cement (Calcibon) were investigated. In addition to unmodified CaP cement discs, 2 other treatments were given to the discs: preincubation in MilliQ and sintering at different temperatures. After primary culture, RBMCs were dropwise seeded on the discs and cultured for 12 days. The samples were evaluated in terms of cell viability, morphology (live and dead assays and scanning electron microscopy (SEM)), cell proliferation (deoxyribonucleic acid (DNA) analyses), early cell differentiation (alkaline phosphatase (ALP) activity), and physicochemical analyses (xray diffraction (XRD)). The live and dead, DNA, and SEM results showed that Calcibon discs without any additional treatment were not supporting osteoblast-like cells in vitro. There were fewer cells, and cell layers were detached from the disc surface. Therefore, different preincubation periods and sintering temperatures were evaluated to improve the cytocompatibility of the CaP cement. Preincubating discs in MilliQ for periods of 1, 4, 8, and 12 weeks resulted in the hydrolysis of a-tri calcium phosphate (TCP) into an apatite-like structure with some b-TCP, as shown with XRD, but the material was not cytocompatible. Sintering the discs between 8008C and 11008C resulted in conversion of a-TCP to b-TCP with some hydroxyapatite and an increase in crystallinity. Eventually, the discs sintered at 11008C achieved better cell attachment, more-abundant cell proliferation, and earlier differentiation than other sintered (6008C, 8008C, and 10008C), preincubated, and unmodified specimens. On basis of our results, we conclude that in vivo results with CaP-based cements do not guarantee in vitro applicability. Furthermore, unmodified Calcibon is not cytocompatible in vitro, although preincubation of the material results in a more-favorable cell response, sintering of the material at 11008C results in the best osteogenic properties. In contrast to in vivo studies, the Calcibon CaP cement is not suitable as a scaffold for cellbased tissue-engineering strategies.

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“…Calcium orthophosphate concretes containing SiO 2 and TiO 2 particles showed a significant (~ 80 -100 MPa) increase in the compressive strength, whilst no change in the mechanical behavior of the formulations was observed when ZrO 2 particles were added [416]. Additional examples of the properties improving comprise addition of calcium silicates [67], polypeptide copolymers [436] and collagen [437][438][439][440][441][442][443]. A strength improvement was found when DCPA and TiO 2 crystals were used as fillers for mechanically activated α-TCP cements [444].…”

Section: Reinforced Self-setting Formulations and Concretesmentioning

confidence: 99%

Self-Setting Calcium Orthophosphate Formulations: Cements, Concretes, Pastes and Putties

Dorozhkin¹

2012

IJMC

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In early 1980s, researchers discovered self-setting calcium orthophosphate cements, which are a bioactive and biodegradable grafting material in the form of a powder and a liquid. Both phases after mixing form a viscous paste that after being implanted sets and hardens within the body as either a non-stoichiometric calcium deficient hydroxyapatite (CDHA) or brushite, sometimes blended with un-reacted particles and other phases. As both CDHA and brushite are remarkably biocompartible and bioresorbable (therefore, in vivo they can be replaced with a newly forming bone), self-setting calcium orthophosphate cements represent a good correction technique of non-weight-bearing bone fractures or defects and appear to be very promising materials for bone grafting applications. Besides, these cements possess an excellent osteoconductivity, molding capabilities, and easy manipulation. Nearly perfect adaptation to the tissue surfaces in bone defects and a gradual bioresorption followed by new bone formation are additional distinctive advantages of calcium orthophosphate cements. Besides, reinforced formulations are available; those are described as calcium orthophosphate concretes. Furthermore, formulations with high viscosity, such as pastes and putties are also known. The discovery of self-setting formulations has opened up a new era in the medical application of calcium orthophosphates; several commercial compositions have already been introduced as a result. Many more formulations are in experimental stages. In this review, an insight into the self-setting calcium orthophosphate formulations, as excellent biomaterials suitable for both dental and bone grafting applications, has been provided.

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Influence of osteocalcin and collagen I on the mechanical and biological properties of Biocement D

Cited by 70 publications

References 13 publications

Preparation of porous apatite granules from calcium phosphate cement

Preparation of porous apatite granules from calcium phosphate cement

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

Self-Setting Calcium Orthophosphate Formulations: Cements, Concretes, Pastes and Putties

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