Influence of osmolality and ions on the activation and characteristics of zebrafish sperm motility

Wilson-Leedy, Jonas G.; Kanuga, M.K.; Ingermann, Rolf L.

doi:10.1016/j.theriogenology.2008.11.006

Cited by 55 publications

(60 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…sperm could not fertilize the eggs when the pH was too low or too high, as similarly reported for Haliotis discus and crossastrea gigas [12]. na + , K + , ca 2+ and mg 2+ are the main cations in aquatic environments that affect the sperm motility of aquatic invertebrates and fishes [1,2,17,19]. In this study, the duration of P. esculenta sperm movement declined significantly in Na + -free artificial seawater compared to natural seawater.…”

Section: Effect Of Different Activation Media On Sperm Motilitysupporting

confidence: 74%

Effects of environmental factors on sperm motility and fertilization rate ofPhascolosoma esculenta

Sheng¹,

Jiang

Jin³

et al. 2014

Acta Biologica Hungarica

View full text Add to dashboard Cite

the study investigated the effects of environmental factors (salinity, pH, ions and activation media) on sperm motility (activation rate, duration of quick movement, and lifespan) and fertilization rate of Phascolosoma esculenta. the results showed that spermatozoa in the coelom and nephridium are able to move quickly. the optimal salinity was 14.64 to 43.35 and the optimal pH was 6.46 to 9.53 for sperm activation and motility, whereas the ranges for fertilization were narrower (18.56 to 30.3 for salinity and 6.46 to 8.61 for pH). of the ions studied, na + was indispensable for sperm motility and fertilization, and ca 2+ and mg 2+ were necessary for fertilization. P. esculenta sperm could not fertilize eggs and have short lifespans in 200 to 600 mmol/l nacl and Kcl solutions. furthermore, they could not be activated or move in 200 to 600 mmol/l cacl 2 , mgso 4 , and sucrose solutions.

show abstract

Section: Effect Of Different Activation Media On Sperm Motilitysupporting

confidence: 74%

Effects of environmental factors on sperm motility and fertilization rate ofPhascolosoma esculenta

Sheng¹,

Jiang

Jin³

et al. 2014

Acta Biologica Hungarica

View full text Add to dashboard Cite

show abstract

“…The osmolarity of extender effect on motility and velocity of spermatozoids there is a close connection between osmolality and ionic composition on the activation or inhibition of sperm motility (Wilson-Leedy et al, 2009;Alavi et al, 2010). Wayman et al (1998) studied extenders with different osmolalities (200, 300 and 400 mOsm/kg) concluded that 200 mOsm/kg was the most suitable for preservation of Sciaenops ocellatus (Linnaeus, 1766) sperm.…”

Section: Dmso (%) Motility Rate (%) Motility Time (S)mentioning

confidence: 99%

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

et al. 2015

View full text Add to dashboard Cite

This study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C -min ), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C Criopreservação do sêmen do ariocó Lutjanus synagris (Linnaeus, 1758) ResumoEste estudo teve a finalidade de desenvolver e avaliar um protocolo de crioconservação do sêmen do ariocó Lutjanus synagris. Para caracterizar o sêmen foram avaliados a taxa de motilidade, a duração da motilidade, a concentração espermática e o espermatócrito. Em seis experimentos foram analisados os efeitos de três diluentes, com distintas composições iônicas e valores de pH distintos, combinados com sete concentrações de dimetilsulfóxido (0; 2,5; 5,0; 7,5; 10,0; 12,5 e 15,0%), cinco velocidades de congelamento (-110, -90, -60, -45 e -30°C/min), nove tempos de equilíbrio (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutos) e cinco proporções de sêmen:diluente (1:1; 1:3; 1:6; 1:10 e 1:20) sobre a taxa de motilidade e a duração da motilidade espermáticas. Posteriormente um teste de fertilização foi realizado para avaliar a viabilidade do sêmen crioconservado. O tratamento que propiciou maior taxa de motilidade e duração da motilidade espermáticas (P<0,05) foi aquele proporcionado pelo emprego do diluente com pH 8,2 com dimetilsulfóxido a 10%, em uma velocidade de congelamento de -60°C/min, com tempo de equilíbrio de 1 minuto e na proporção de 1:3 (v/v). O sêmen crioconservado apresentou taxa de fertilização superior a 69% validando o presente protocolo para o ariocó. A crioconservação do sêmen do ariocó é uma alternativa viável, sendo possível manter uma apropriada qualidade espermática.

show abstract

“…This shows the importance of using adequate osmolality to activate fish species spermatozoa. Many authors suggested that, as for several fresh water fish species, the primary signal for initiation of sperm motility in zebrafish Danio rerio (Hamilton, 1822), is a change in osmolality (Alavi and Cosson 2006;Wilson-Leedy et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…As these conditions depolarize the cell membrane may affect the capacity of flagella motility (Alavi and Cosson, 2006;Morisawa and Suzuki, 1980). Once motility of spermatozoa has been initiated, its duration is typically short, often less than one minute in fresh water species, with hypotonicityassociated cell swelling and lysis as a limiting factor (Alavi and Cosson, 2006;Wilson-Leedy et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

Saedeghi¹,

Núñez²,

Soler³

et al. 2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

In the present work, the effects of the activation media with different osmolality and storage conditions on sperm motility and kinetic parameters over time after activation, using CASA system (ISAS®) in Danio rerio were analysed. The effect of pooled and individual ejaculate samples was also studied. Spermatozoids activated with high osmolality medium showed higher values in motility parameters and kinematic parameters in comparison with low osmolality medium. Respect to the individual ejaculates or the pool, although significant differences were observed in some parameters, no relevant changes were observed in total motility neither in kinetic parameter. Total motility and velocity of sperm showed significant lower values after sperm storage for 24 h at 4 ºC.

show abstract

Influence of osmolality and ions on the activation and characteristics of zebrafish sperm motility

Cited by 55 publications

References 31 publications

Effects of environmental factors on sperm motility and fertilization rate ofPhascolosoma esculenta

Effects of environmental factors on sperm motility and fertilization rate ofPhascolosoma esculenta

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

Untitled

Contact Info

Product

Resources

About