Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion

Fernandes, Raphaella Barcellos; Polo, Ana Bárbara; Rocha, Vinícius Novaes; Vitral, Robert Willer Farinazzo; Apolônio, Ana Carolina Morais; Campos, Márcio José da Silva

doi:10.1016/j.sdentj.2022.03.008

Cited by 8 publications

(10 citation statements)

References 30 publications

(41 reference statements)

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“…These results can be attributed to the fact that the ceramic brackets show an increased surface than the self-ligating bracket system thereby attracting more plaque biofilm. (19) The eruption of the teeth during the first year leads to colonization by Streptococcus mutans and Streptococcus sanguis. These bacteria require a non-desquamating (nonepithelial) surface in order to colonize.…”

Section: Discussionmentioning

confidence: 99%

Microbial Analysis of Plaque Biofilm In Subjects Undergoing Orthodontic Treatment With Different Bracket Systems

2023

JPTCP

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Objectives: To identify microorganisms isolated from patients wearing different types of fixed orthodontic appliances and evaluate the resistance of isolated bacterial strains to different antimicrobials. Materials and Methods: Nineteen patients indicated to undergo fixed orthodontic treatment were chosen for the study and were divided into 3 groups -7 patients in group 1 ( conventional metal brackets), 6 patients in group 2 (conventional ceramic brackets), and 6 patients in group 3 (Passive self-ligating metal system). Plaque biofilm formed around orthodontic brackets was collected, samples were plated onto brain heart infusion agar and mitis salivarius agar and were incubated. Similarly, samples were collected in blood broth and were streaked onto blood agar to evaluate the anaerobic colony counts. Results: Maximum aerobic and anaerobic bacterial count was seen in group 2 and least in group 3. Most prevalent microbial genera were streptococcus mutans showing predominantly sensitive strains with one strain from Group 3 which showed resistance against amikacin, cefazolin, cefixime, and cefazolin. Conclusions: The number of aerobic and anaerobic CFU/ml is higher on the surface of ceramic brackets when compared to the conventional metal or the Passive self-ligating system. Streptococcus mutans was most prevalent bacterial strain amongst all aerobic bacteria isolated from all three groups.

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Section: Discussionmentioning

confidence: 99%

Microbial Analysis of Plaque Biofilm In Subjects Undergoing Orthodontic Treatment With Different Bracket Systems

2023

JPTCP

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“…Then, 2 mL of the bacterial suspension was taken and added to each sample in such a manner to completely cover it with bacterial suspension. The samples were placed inside the CO 2 incubator at 37 °C for 24 h of incubation [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

“…To remove the planktonic bacteria, the coated brackets were cleaned two times with 0.1 M phosphate buffered saline, pH 7, 20 µl of the suspension was inoculated into the microwells of the microtiter plate, which were aseptically and individually placed into a 96-well microplate. A quick read was obtained at 450 nm using a Biotek spectrophotometer [ 28 ]. Bacterial viability was assessed by counting colony forming units (CFU).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the surface characteristics and antibacterial properties of Titanium dioxide nanotube and methacryloyloxyethylphosphorylcholine (MPC) coated orthodontic brackets-a comparative invitro study

Rao,

Ashith,

Suman

et al. 2024

Clin Oral Invest

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Objectives White spot lesions are the most common iatrogenic effect observed during orthodontic treatment. This study aimed to compare the surface characteristics and antibacterial action of uncoated and coated orthodontic brackets. Materials and methods Sixty commercially available stainless steel brackets were coated with TiO2 nanotubes and methacryloyloxyethylphosphorylcholine. The sample was divided into Group 1: uncoated orthodontic brackets, Group 2: Stainless steel brackets with TiO2 nanotubes coating, Group 3: Stainless steel brackets with methacryloyloxyethylphosphorylcholine coating, and Group 4: Stainless steel brackets with TiO2 nanotubes combined with methacryloyloxyethylphosphorylcholine coating. Surface characterization was assessed using atomic force microscopy and scanning electron microscopy. Streptococcus mutans was selected to test the antibacterial ability of the orthodontic brackets, total bacterial adhesion and bacterial viability were assessed. The brackets were subjected to scanning electron microscopy to detect the presence of biofilm. Results The surface roughness was the greatest in Group 1 and least in Group 2 followed by Group 4 and Group 3 coated brackets. The optical density values were highest in Group 1 and lowest in Group 4. Comparison of colony counts revealed high counts in Group 1 and low counts in Group 4. A positive correlation between surface roughness and colony counts was obtained, however, was not statistically significant. Conclusions The coated orthodontic brackets exhibited less surface roughness than the uncoated orthodontic brackets. Group 4 coated orthodontic brackets showed the best antibacterial properties. Clinical relevance Coated orthodontic brackets prevent adhesion of streptococcus mutans and reduces plaque accumulation around the brackets thereby preventing formation of white spot lesions during orthodontic treatment.

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“…A quick read was obtained at 450 nm using a Biotek spectrophotometer. 2 Bacterial viability was assessed by counting colony forming units (CFU). After the samples were incubated, the brackets were once again cleaned with phosphate buffered saline (PBS) to eliminate the planktonic cells.…”

Section: Methodsmentioning

confidence: 99%

“…The CFU value in the brackets was calculated as the number of CFUs counted X dilution factor X 10. 2 The brackets were separated from the teeth and subjected to SEM to detect the presence of bio lm. Scanning electron microscopy with magni cation set at 1000 x was used to evaluate the formation of bio lm and adhesion to orthodontics brackets.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the surface characteristics and antibacterial properties of TiO2 nanotube and Methacryloyloxyethylphosphorylcholine (MPC) coated orthodontic brackets-A Comparative Invitro study

Rao,

Suman

et al. 2023

Preprint

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Objectives White spot lesions is the most common iatrogenic effect observed post orthodontic treatment. This study aimed to compare the surface characteristics such as morphology, roughness, and antibacterial action of uncoated and coated orthodontic brackets. 1 Materials and Methods Sixty commercially available stainless steel brackets were coated with TiO2 nanotubes and methacryloyloxyethylphosphorylcholine (MPC). The surface roughness was assessed with AFM and surface topography using SEM. Streptococcus mutans was selected to test the antibacterial ability of the orthodontic brackets. Measurement of total bacterial adhesion was performed using the optical density (OD) method. Bacterial viability was assessed by counting colony forming units (CFU). The brackets were subjected to SEM to detect the biofilm. 2 Results The computed results indicate that surface roughness was the greatest in uncoated orthodontic brackets. TiO2NT-coated brackets exhibited the least surface roughness, followed by TiO2 NT + MPC- and MPC-coated brackets. The OD values were highest in uncoated orthodontic brackets with TiO2 NT + MPC having the lowest OD values. Comparison of colony counts showed the highest counts in uncoated orthodontic brackets and the lowest counts in the TiO2 NT + MPC group. A positive correlation between surface roughness and colony counts was obtained but was not statistically significant. Conclusions The coated orthodontic brackets exhibited less surface roughness than the uncoated orthodontic brackets. TiO2 NT+MPC-coated orthodontic brackets showed the best antibacterial properties. Clinical Relevance: Coated orthodontic brackets prevent adhesion of streptococcus mutans onto its surface and reduces plaque accumulation around the brackets thereby reducing the formation of white spot lesions post orthodontic treatment.

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Influence of orthodontic brackets design and surface properties on the cariogenic Streptococcus mutans adhesion

Cited by 8 publications

References 30 publications

Microbial Analysis of Plaque Biofilm In Subjects Undergoing Orthodontic Treatment With Different Bracket Systems

Microbial Analysis of Plaque Biofilm In Subjects Undergoing Orthodontic Treatment With Different Bracket Systems

Evaluation of the surface characteristics and antibacterial properties of Titanium dioxide nanotube and methacryloyloxyethylphosphorylcholine (MPC) coated orthodontic brackets-a comparative invitro study

Evaluation of the surface characteristics and antibacterial properties of TiO2 nanotube and Methacryloyloxyethylphosphorylcholine (MPC) coated orthodontic brackets-A Comparative Invitro study

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