Influence of organic solvents in coelectroosmotic capillary electrophoresis of phenols

Masselter, Tom; Zemann, Andreas

doi:10.1021/ac00102a005

Cited by 111 publications

(58 citation statements)

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“…For the analysis of anionic species, the EOF is typically reversed by the addition of cationic additives such as polymers or quaternary amines in the BGE [6,[22][23][24]. Using this approach, anions migrate with the EOF, and thus rapid separations are achieved.…”

Section: Introductionmentioning

confidence: 99%

Semipermanent capillary coatings in mixed organic–water solvents for CE

2007

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This report describes the creation of semipermanent capillary coatings that are compatible with organic-water solvent systems in CE. The coatings are created by simply rinsing the fused-silica capillary with long double-chain cationic surfactants, such as dimethyl-ditetradecyl ammonium bromide (2C(14)DAB), dihexadecyldimethyl ammonium bromide (2C(16)DAB), and dimethyldioctadecyl ammonium bromide (2C(18)DAB). These surfactants generate semipermanent bilayer coatings on the capillary surface, which display a high degree of stability in buffers containing up to 60% v/v of organic solvents, such as methanol and ACN. The coating stability increases with increasing hydrophobicity of the surfactant, i.e., with increasing chain length. For instance, the EOF changes by only 1.2% in a 2C(18)DAB-coated capillary after 130 capillary volumes of rinsing with 60% v/v methanol containing buffer. The bilayer coatings allow separations to be performed without the need to regenerate the coating between runs or to maintain the EOF modifier in the run buffer. Rapid separations (<2 min) of anions and basic drugs with migration time reproducibility of less than 0.5% RSD and efficiencies of 0.4-0.6 million plates/m are obtained. In addition, selectivity changes for small anions and cationic drugs are also observed when the organic solvent content is adjusted.

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Section: Introductionmentioning

confidence: 99%

Semipermanent capillary coatings in mixed organic–water solvents for CE

2007

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“…However, under these conditions the other non-dissociated phenols interact strongly with the capillary wall and the peaks in the chromatogram are broad and tailing. For the light CP's (mono-and di-substituted isomers, peak numbers: 8,11,13,14,15,16,17,18,19), the best separation can be achieved with a buffer-pH close to their average pK a value. At pH 8.2 the separation sequence follows exactly their pK a values (Figure 2a) but the least acidic phenol still coincides with the EOF front.…”

Section: Resultsmentioning

confidence: 99%

Separation of Chlorophenols by Capillary Zone Electrophoresis. The Influence of pH of the Electrophoretic Buffer on Selectivity

Liu

Frank

1998

J. High Resol. Chromatogr.

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“…Indeed, the expression (6) used in such corrections does not take into account the influences on the current due to the modifications in the diffuse double layer. However, the effects on the status of the capillary surface have been shown in conditions comparable to high analyte concentrations such as the addition of organic cosolvents to the electrolyte [28,29].…”

Section: Determination Of the Association Constant And Discussionmentioning

confidence: 99%

Capillary Electrophoresis as an Efficient Tool in the Determination of Low Association Constants between Polyhydroxy Compound and Samarium Cation

Henry

1999

J. High Resol. Chromatogr.

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Influence of organic solvents in coelectroosmotic capillary electrophoresis of phenols

Cited by 111 publications

References 1 publication

Semipermanent capillary coatings in mixed organic–water solvents for CE

Semipermanent capillary coatings in mixed organic–water solvents for CE

Separation of Chlorophenols by Capillary Zone Electrophoresis. The Influence of pH of the Electrophoretic Buffer on Selectivity

Capillary Electrophoresis as an Efficient Tool in the Determination of Low Association Constants between Polyhydroxy Compound and Samarium Cation

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