Influence of organic nutrients and cocultures on the competitive behavior of 1,2-dichloroethane-degrading bacteria

Wijngaard, Arjan J. van den; Kleij, R G van der; Doornweerd, R E; Janssen, Dick B.

doi:10.1128/aem.59.10.3400-3405.1993

Cited by 19 publications

(3 citation statements)

References 17 publications

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“…In addition, gas-phase enzymatic reactions held irreplaceable merits in the processing of gaseous substrates. For instance, the detection and detoxification of nerve agents, the decontamination of volatile pollutants, and the biosynthesis of ammonia from N 2 14,15 . Pioneering studies have shown that enzymes (e.g.…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide enabled long-term enzymatic transesterification in an anhydrous gas flux

Gong

et al. 2019

Nat Commun

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Gas-phase enzymatic catalysis has been long pursued but not yet utilized in industrial processes due to many limitations. Herein, we report a hydroxyl-rich graphene oxide (GO) aerogel that can preserve the enzymatic activity and stability in an anhydrous gas flow by providing a water-like microenvironment. Lipase immobilized in the GO aerogel exhibits a 5 to 10-fold increase in apparent activity than the lyophilized lipase powder in transesterification of geraniol and vinyl acetate in the gas phase and maintains the initial activity for more than 500 h. The solid-state circular dichroism measurement confirms that the lipase keeps its native conformation in the aerogel, and the thermogravimetric analysis shows that water molecules essential for the lipase activity can be replaced by the hydroxyl groups at the GO surface. The versatility of this method is demonstrated for two other lipases with different structures, promising unprecedented applications of enzyme-GO aerogels to gas-phase enzymatic catalysis.

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Section: Introductionmentioning

confidence: 99%

Graphene oxide enabled long-term enzymatic transesterification in an anhydrous gas flux

Gong

et al. 2019

Nat Commun

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“…However, A. aquaticus AD25, as well as X. autotrophicus GJ10 and Pseudomonas sp. strain DE2, require vitamins for optimal growth (16,21). Therefore, we set out to isolate DCA-degrading bacteria with a high affinity for DCA but without the dependency on vitamins for optimal growth.…”

mentioning

confidence: 99%

Monooxygenase-Mediated 1,2-Dichloroethane Degradation by Pseudomonas sp. Strain DCA1

Hage

Hartmans

1999

Appl Environ Microbiol

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A bacterial strain, designated Pseudomonas sp. strain DCA1, was isolated from a 1,2-dichloroethane (DCA)-degrading biofilm. Strain DCA1 utilizes DCA as the sole carbon and energy source and does not require additional organic nutrients, such as vitamins, for optimal growth. The affinity of strain DCA1 for DCA is very high, with aKm value below the detection limit of 0.5 μM. Instead of a hydrolytic dehalogenation, as in other DCA utilizers, the first step in DCA degradation in strain DCA1 is an oxidation reaction. Oxygen and NAD(P)H are required for this initial step. Propene was converted to 1,2-epoxypropane by DCA-grown cells and competitively inhibited DCA degradation. We concluded that a monooxygenase is responsible for the first step in DCA degradation in strain DCA1. Oxidation of DCA probably results in the formation of the unstable intermediate 1,2-dichloroethanol, which spontaneously releases chloride, yielding chloroacetaldehyde. The DCA degradation pathway in strain DCA1 proceeds from chloroacetaldehyde via chloroacetic acid and presumably glycolic acid, which is similar to degradation routes observed in other DCA-utilizing bacteria.

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“…strain GJi grew more stably on 1,2-dichloroethane than did strain GJ10 alone, since GJi fulfilled the requirement of strain GJ10 for biotin and GJ1 degraded the toxic compounds that accumulated from the dichloroethane-utilizing strain GJ10 under suboptimal conditions (521).…”

Section: Application Of Microbial Systems In Environmental Protectionmentioning

confidence: 98%

Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.

Fetzner

Lingens

1994

Microbiological Reviews

260

134

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hydraulic and heat transfer fluids, plasticizers, and intermediates for chemical syntheses. Because of their toxicity, bioconcentration, and persistence, the ubiquitous distribution of halogenated compounds in the biosphere has caused public concern over the possible effects on the quality of life. Concerning toxicity, an important problem is the diversity of toxic compounds and by-products in many formulations of industrial products. This problem applies to mixtures of polychlorinated biphenyls (PCBs) or chlorinated terpenes, poly-641

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Influence of organic nutrients and cocultures on the competitive behavior of 1,2-dichloroethane-degrading bacteria

Cited by 19 publications

References 17 publications

Graphene oxide enabled long-term enzymatic transesterification in an anhydrous gas flux

Graphene oxide enabled long-term enzymatic transesterification in an anhydrous gas flux

Monooxygenase-Mediated 1,2-Dichloroethane Degradation by Pseudomonas sp. Strain DCA1

Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.

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