Influence of Onabotulinumtoxin A on testes of the growing rat

Breikaa, Randa M.; Mosli, Hisham A.; Abdel-Naim, Ashraf B.

doi:10.1002/jbt.21828

Cited by 3 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Botulinum toxins (BoNTs) are a class of fatal proteins, mainly produced by Clostridium botulinum , which causes muscle paralysis as they act by binding presynaptically to high-affinity recognition sites on the cholinergic nerve terminals and effectively prevents the exocytosis of ACh vesicles at the neuromuscular junctions [ 8 – 10 ]. While a trace amount of purified BoNT has been identified to yield long-lasting anti-ageing therapeutic benefits and mitigating neuromuscular defects in various illnesses [ 8 , 9 , 11 ], some toxicological reports indicated that cremasteric injection of BoNT (10–40 U/kg) induces cell death and impairs spermiogenesis in the testis of experimental animals [ 12 , 13 ]. However, a recent report revealed that intrascrotal injection of BoNT in adult men did not induce any obvious adverse effect [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Single injection of very mild dose botulinum toxin in the vastus lateralis improves testicular spermatogenesis and sperm motility in ageing experimental mice

et al. 2022

View full text Add to dashboard Cite

Background Botulinum toxin (BoNT) is a widely used therapeutic agent that blocks the excessive release of acetylcholine at the neuromuscular junction. Previously, repeated intracremasteric injections and slight overdose of BoNT have been reported to induce adverse effects in the testicular parameter of experimental rodents. However, a mild dose of BoNT is highly beneficial against skin ageing, neuromuscular deficits, overactive urinary bladder problems, testicular pain and erectile dysfunctions. Considering the facts, the possible therapeutic benefits of BoNT on the testis might be achieved at a very minimal dosage and via a distal route of action. Therefore, we revisited the effect of BoNT, but with a trace amount injected into the vastus lateralis of the thigh muscle, and analyzed histological parameters of the testis, levels of key antioxidants and sperm parameters in ageing experimental mice. Results Experimental animals injected with 1 U/kg bodyweight of BoNT showed enhanced spermatogenesis in association with increased activities of key antioxidants in the testis, leading to enhanced amount of the total sperm count and progressive motility. Conclusions This study signifies that a mild intramuscular dose of BoNT can be considered as a potent treatment strategy to manage and prevent male infertility.

show abstract

Section: Introductionmentioning

confidence: 99%

Single injection of very mild dose botulinum toxin in the vastus lateralis improves testicular spermatogenesis and sperm motility in ageing experimental mice

et al. 2022

View full text Add to dashboard Cite

show abstract

“…However, few toxicological reports indicated that cremasteric injection of botulinum toxin (10, to 40 U/kg) induces cell death and impairs spermiogenesis in the testis of experimental animals [12,13], while a recent report indicates that intrascrotal injection of botulinum toxin in adult male did not induce any adverse effect [14]. Nevertheless, ample scientific evidence unequivocally points towards the positive episodes of a very mild dose of therapeutic botulinum toxin such as BOTOX® against various clinical complications including movement disorders, cognitive deficit and behavioral abnormalities [8,9,11,[15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Single injection of very mild dose BOTOX in the vastus lateralis improves testicular spermatogenesis and sperm motility in ageing experimental mice

Radhakrishnan

Ravichandran

Sukesh

et al. 2021

Preprint

View full text Add to dashboard Cite

Acetylcholine (ACh), a key neurochemical messenger that plays key roles in neuroplasticity and muscle contraction. While ACh is important for the physiological function of the testis, abnormal levels of ACh cause testicular atrophy and male infertility. BOTOX is a therapeutic form of the botulinum neurotoxin that blocks the excessive release of ACh at the neuromuscular junction. Previously, repeated intracremasteric injections and slight overdose of BOTOX have been reported to induce adverse effects in the testicular parameter of experimental rodents. However, a mild dose of BOTOX is highly beneficial against skin ageing, neurological deficits, overactive urinary bladder problems, testicular pain and erectile dysfunctions. Considering the facts, the possible therapeutic benefit of BOTOX on the testis might be achieved via its minimal dose and indirect mode of action rather than repeated high quantity in the local supply. Therefore, we revisited the effect of BOTOX but with a trace amount injected into the vastus lateralis of the thigh muscle, and analyzed histological parameters of testis and quality of semen in ageing experimental mice. Experimental animals injected with 1 U/kg bodyweight of BOTOX showed enhanced spermatogenesis in associations with increased activities of key antioxidants in the testis, leading to increased total sperm count and motility. This study signifies that a mild intramuscular dose of BOTOX can be considered as a potential treatment strategy to manage and prevent male infertility.

show abstract

Intrascrotal injection of botulinum toxin A, a male genital aesthetic demand: Technique and limits

Ramelli

Brault

Tierny

et al. 2020

Progrès en Urologie

View full text Add to dashboard Cite

Influence of Onabotulinumtoxin A on testes of the growing rat

Cited by 3 publications

References 21 publications

Single injection of very mild dose botulinum toxin in the vastus lateralis improves testicular spermatogenesis and sperm motility in ageing experimental mice

Single injection of very mild dose botulinum toxin in the vastus lateralis improves testicular spermatogenesis and sperm motility in ageing experimental mice

Single injection of very mild dose BOTOX in the vastus lateralis improves testicular spermatogenesis and sperm motility in ageing experimental mice

Intrascrotal injection of botulinum toxin A, a male genital aesthetic demand: Technique and limits

Contact Info

Product

Resources

About