Influence of Nutritional and Climatic Conditions on Mycelial Growth of Three Oyster Mushroom Strains

Aziz, Norhan H. Abdel; Yousef, Nahed S.; El-Haddad, M. E.; El-Tayeb, T. S.

doi:10.21608/ajs.2018.28368

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…It was previously found that this medium is also best suited for mycelial growth of other strains of P. ostreatus under different study [ 14 , 21 – 23 ]. In contrast to our observations, the best mycelial growth of P. ostreatus was observed in MEA [ 24 , 25 ].…”

Section: Discussioncontrasting

confidence: 99%

Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities

Krupodorova,

Barshteyn,

Tsygankova

et al. 2024

BMC Biotechnol

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Background The production of Pleurotus ostreatus mycelium as a promising object for use in food and other industries is hampered by a lack of information about the strain-specificity of this fungus mycelium growth and its acquisition of various biological activities. Therefore, this research aimed to investigate mycelial growth of different P. ostreatus strains on varies solid and liquid media as well as to evaluate strains antagonistic, antibacterial, antiradical scavenging activities, and total phenolic content. Results Potato Dextrose Agar medium was suitable for all strains except P. ostreatus strain 2460. The best growth rate of P. ostreatus 2462 strain on solid culture media was 15.0 ± 0.8 mm/day, and mycelia best growth on liquid culture media—36.5 ± 0.2 g/l. P. ostreatus strains 551 and 1685 were more susceptible to positive effect of plant growth regulators Ivin, Methyur and Kamethur. Using of nutrient media based on combination of natural waste (amaranth flour cake and wheat germ, wheat bran, broken vermicelli and crumbs) has been increased the yield of P. ostreatus strains mycelium by 2.2–2.9 times compared to the control. All used P. ostreatus strains displayed strong antagonistic activity in co-cultivation with Aspergillus niger, Candida albicans, Issatchenkia orientalis, Fusarium poae, Microdochium nivale in dual-culture assay. P. ostreatus 2462 EtOAc mycelial extract good inhibited growth of Escherichia coli (17.0 ± 0.9 mm) while P. ostreatus 2460 suppressed Staphylococcus aureus growth (21.5 ± 0.5 mm) by agar well diffusion method. The highest radical scavenging effect displayed both mycelial extracts (EtOH and EtOAc) of P. ostreatus 1685 (61 and 56%) by DPPH assay as well as high phenolic content (7.17 and 6.73 mg GAE/g) by the Folin-Ciocalteu’s method. The maximal total phenol content (7.52 mg GAE/g) demonstrated of P. ostreatus 2461 EtOH extract. Conclusions It is found that the growth, antibacterial, antiradical scavenging activity as well as total phenolic content were dependent on studied P. ostreatus strains in contrast to antagonistic activity. The proposed culture mediums of natural waste could be an alternative to commercial mediums for the production mycelial biomass of P. ostreatus strains.

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Section: Discussioncontrasting

confidence: 99%

Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities

Krupodorova,

Barshteyn,

Tsygankova

et al. 2024

BMC Biotechnol

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“…It was previously found that this medium is also best suited for mycelial growth of other strains of P. ostreatus under different study [14,21,22,23]. In contrast to our observations, the best mycelial growth of P. ostreatus was observed in MEA [24,25].…”

Section: Discussioncontrasting

confidence: 79%

Strain-specific Features of Pleurotus Osreatus Growth in Vitro and Some of Its Biological Activities

Tetiana,

Victor,

Victoria

et al. 2023

Preprint

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Pleurotus ostreatus mycelium is widely used as a base for a variety of food and other products. This study focuses on P. ostreatus strains, increasing the production of their mycelium, studying some valuable biological properties in order to select the best strain. Using of nutrient media based on combination of natural waste (amaranth flour cake and wheat germ, wheat bran, chopped vermicelli and crumbs) has been increased the yield of P. ostreatus strains mycelium by 2.2–2.9 times compared to the control. P. ostreatus strains 551 and 1685 were more receptive to positive assimilation of all investigated growth regulators Ivin, Methyur and Kamethur used in concentrations ranged from 10− 6 to 10− 9 M (mol/l). The highest radical scavenging effect displayed by P. ostreatus 1685 (61 and 56%) and both of its mycelial extracts (EtOH and EtOAc) showed high phenolic content (7.17 and 6.73 mg of GAE/g). The maximal TPC (7.52 mg of GAE/g) demonstrated EtOH extract of P. ostreatus 2461. P. ostreatus 2462 is a high yielding strain that showed the maximum mycelial growth (15.0 ± 0.8 mm/day on PDA) on solid and liquid media and its EtOAc mycelial extract good inhibited growth of Escherichia coli (17.0 ± 0.9 mm). Mediocre growth had P. ostreatus 2460 but it can success suppressed Staphylococcus aureus growth (21.5 ± 0.5 mm). All used P. ostreatus strains displayed strong antagonistic activity in co-cultivation with Aspergillus niger, Candida albicans, Issatchenkia orientalis, Fusarium poae, Microdochium nivalе. Among used strains, P. ostreatus 1685 is quite tolerant to growth conditions and the most promising culture.

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Biology, morphology, and phylogeny of some strains of the Pleurotus eryngii species complex

Bisko

Lomberg

Mykchaylova

et al. 2022

ARCH BIOL SCI BELGRA

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Effective methods of preserving the gene pool of valuable edible and medicinal mushrooms are to maintain them in in vitro culture collections and to correctly identify and verify the preserved strains. It is necessary to consider not only the results of molecular genetic studies but also cultural, morphological and physiological characteristics as additional criteria. This article presents data on the colony and mycelial morphology, growth characteristics and temperature tolerance, and phylogenetical placement of four strains of edible and medicinal mushroom from the P. eryngii species complex received into the IBK Mushroom Collection as P. nebrodensis strains. All the studied strains are mesophiles with the fastest growth rate of 11.0 mm/day at 26?? and a lethal temperature of 40??. In addition to common anastomoses, mycelial strands and clamp connections, the vegetative mycelium of the studied strains formed single colorless round excretory cells on the lateral hyphal ramifications. All cultures were able to form primordia and fruit bodies on agar media. Phylogenetic analysis suggests that all four strains do not belong to P. nebrodensis, but two of them, IBK 1947, 2035 are P. eryngii var. ferulae, and two strains, IBK 1855 and 1927, belong to P. tuoliensis (P. eryngii var. tuoliensis).

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Influence of Nutritional and Climatic Conditions on Mycelial Growth of Three Oyster Mushroom Strains

Cited by 3 publications

References 4 publications

Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities

Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities

Strain-specific Features of Pleurotus Osreatus Growth in Vitro and Some of Its Biological Activities

Biology, morphology, and phylogeny of some strains of the Pleurotus eryngii species complex

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