Influence of nutrient‐limited growth on pathogenesis‐associated outer membrane proteins of Yersinia enterocolitica

Abstract

From studies based on batch culture, it has been postulated that the expression of the virulence-associated proteins of Yersinia spp. is controlled by temperature and Ca2+, such that these proteins are synthesized only at the higher temperature (37 degrees C) and calcium-scarce conditions of the intracellular environment. It was found, however, that in Yersinia enterocolitica one of these proteins (140 kDa) is not synthesized at submaximal growth rates under any of the relevant conditions, and that another of the implicated proteins (34 kDa), is synthesized even at 28 degrees C during nutrient-limited growth. Thus, temperature and Ca2+ influence the synthesis of these proteins differently under growth conditions that better approximate the natural environments than do batch cultures.

Adhesion of Pseudomonas aeruginosa to silicone rubber in a parallel plate flow chambe in the absence and presence of nutrient broth

“…Physico-chemical studies on microbial adhesion to surfaces are typically carried out with organisms suspended in buffers to avoid microbial growth or other associated complications (Van Loosdrecht et al ., 1990). In vitro studies have identified changes in antimicrobial efficacy (Evans et al ., 1990, 1991), protein expression (Harakeh & Matin, 1989), cell-surface hydrophobicity (Ljungh & Wad-strӧm, 1995) and adhesion (Fletcher, 1976; Zeng et al ., 1994), depending upon growth conditions. Alternatively, adhesion studies sometimes involve optimal growth conditions.…”