Influence of nitric oxide synthase or cyclooxygenase inhibitors on cannabinoids activity in streptozotocin-induced neuropathy

Bujalska‐Zadrożny, Magdalena; Cordé, Anna de; Pawlik, Karolina

doi:10.1016/j.pharep.2014.08.023

Cited by 15 publications

(9 citation statements)

References 43 publications

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“…According to the authors, the use of a specific nNOS inhibitor (7-Ni) and a relatively selective inhibitor of iNOS (L-NIL) showed a positive impact over the analgesic effect of two cannabinoid receptors agonists. Such finding suggests that CBD might not only modulate the expression and activity of NOS isoforms, but also have an inhibitory effect on the analgesia and anti-inflammatory pathways activated by cannabinoids agonists ( 36 ). In this study, we observed the reduction of nitrite/nitrate concentration in the GCBD group in comparison to the G group; values were similar to the groups C and CBD, which indirectly shows the reduction of NO concentration in the treated group in comparison to the diseased non-treated group.…”

Section: Discussionmentioning

confidence: 99%

Maternal administration of cannabidiol promotes an anti-inflammatory effect on the intestinal wall in a gastroschisis rat model

Callejas

Figueira

Gonçalves

et al. 2018

Braz J Med Biol Res

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Gastroschisis (GS) is an abdominal wall defect that results in histological and morphological changes leading to intestinal motility perturbation and impaired absorption of nutrients. Due to its anti-inflammatory, antioxidant, and neuroprotective effects, cannabidiol (CBD) has been used as a therapeutic agent in many diseases. Our aim was to test the effect of maternal CBD in the intestine of an experimental model of GS. Pregnant rats were treated over 3 days with CBD (30 mg/kg) after the surgical induction of GS (day 18.5 of gestation) and compared to controls. Fetuses were divided into 4 groups: 1) control (C); 2) C+CBD (CCBD); 3) gastroschisis (G), and 4) G+CBD (GCBD). On day 21.5 of gestation, the fetuses were harvested and evaluated for: a) body weight (BW), intestinal weight (IW), and IW/BW ratio; b) histometric analysis of the intestinal wall; c) immunohistochemically analysis of inflammation (iNOS) and nitrite/nitrate level. BW: GCBD was lower than CCBD (P<0.005), IW and IW/BW ratio: GCBD was smaller than G (P<0.005), GCBD presented lower thickness in all parameters compared to G (P<0.005), iNOS and nitrite/nitrate were lower concentration in GCBD than to G (P<0.005). Maternal use of CBD had a beneficial effect on the intestinal loops of GS with decreased nitrite/nitrate and iNOS expression.

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Section: Discussionmentioning

confidence: 99%

Maternal administration of cannabidiol promotes an anti-inflammatory effect on the intestinal wall in a gastroschisis rat model

Callejas

Figueira

Gonçalves

et al. 2018

Braz J Med Biol Res

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“…Several DN treatments targeting COX-2 have been reported, including rutin in combination with nimesulide (55), Juglans regia L. leaf extract (56) and curcumin (57). As a known inhibitor of COX-2, CXB has been demonstrated to reduce neuropathic pain caused by DN in rats (27,58). However, to the best of our knowledge, the present study is the first to reveal a possible mechanism for CXB in the treatment of DN.…”

Section: Discussionmentioning

confidence: 74%

Celecoxib ameliorates diabetic neuropathy by decreasing apoptosis and oxidative stress in dorsal root ganglion neurons via the miR‑155/COX‑2 axis

Cheng

Zhao

et al. 2021

Exp Ther Med

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Celecoxib (CXB) is the only clinical cyclooxygenase-2 (COX-2) inhibitor. Oral administration of CXB in experimental diabetic mice effectively relieved the symptoms of diabetic neuropathy (DN); however, the molecular mechanism remains unclear. The present study aimed to investigate the potential molecular mechanisms of CXB in the treatment of DN. An in vitro cellular model of DN was produced by stimulating dorsal root ganglion (DRG) neurons with high glucose. Cell viability and apoptosis were assessed by Cell Counting Kit-8 assays and flow cytometry, respectively. Reactive oxygen species (ROS) kits, ELISA kits and western blotting were used to determine oxidative cellular damage. The expression level of microRNA (miR)-155 was analyzed by reverse transcription-quantitative PCR. The starBase database and dual-luciferase assays were performed to predict and determine the interaction between miR-155 and COX-2. Protein expression of neurotrophic factors, oxidative stress-related proteins and COX-2 were analyzed by western blotting. Incubation with high glucose led to a decrease in DRG neuron cell viability, facilitated apoptosis, downregulated NGF and BDNF expression, increased ROS and MDA generation and decreased SOD activity. Treatment with CXB significantly protected DRG neurons against high glucose-evoked damage. CXB promoted the expression of miR-155 and COX-2 was revealed to be a direct target of miR-155. Inhibition of COX-2 enhanced the protective effect of CXB on DRG neurons and that treatment with an miR-155 inhibitor partially rescued this effect. The present study demonstrated the involvement of the miR-155/COX-2 axis in the protective effect of CXB against high glucose-induced DN.

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“…( 45 ) have shown that COX-2 can lead to destruction of pancreatic tissues in diabetes, suggesting that regulating COX-2 pathway is a potential therapeutic strategy to control diabetic peripheral neuropathy. Several studies have shown that COX-2 and iNOS inhibitors can effectively alleviate diabetic neuropathic pain in STZ-induced neuropathy ( 46 ). COX-2 and iNOS also contribute to STZ-induced diabetic hyperalgesia.…”

Section: Resultsmentioning

confidence: 99%

Protective effects of Tat-DJ-1 protein against streptozotocin-induced diabetes in a mice model

Yeo

Shin

et al. 2018

BMB Rep.

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A major feature of type 1 diabetes mellitus (T1DM) is hyperglycemia and dysfunction of pancreatic β-cells. In a previous study, we have shown that Tat-DJ-1 protein inhibits pancreatic RINm5F β-cell death caused by oxidative stress. In this study, we examined effects of Tat-DJ-1 protein on streptozotocin (STZ)-induced diabetic mice. Wild type (WT) Tat-DJ-1 protein transduced into pancreas where it markedly inhibited pancreatic β-cell destruction and regulated levels of serum parameters including insulin, alkaline phosphatase (ALP), and free fatty acid (FFA) secretion. In addition, transduced WT Tat-DJ-1 protein significantly inhibited the activation of NF-κB and MAPK (ERK and p38) expression as well as expression of COX-2 and iNOS in STZ exposed pancreas. In contrast, treatment with C106A mutant Tat-DJ-1 protein showed no protective effects. Collectively, our results indicate that WT Tat-DJ-1 protein can significantly ameliorate pancreatic tissues in STZ-induced diabetes in mice.

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Influence of nitric oxide synthase or cyclooxygenase inhibitors on cannabinoids activity in streptozotocin-induced neuropathy

Cited by 15 publications

References 43 publications

Maternal administration of cannabidiol promotes an anti-inflammatory effect on the intestinal wall in a gastroschisis rat model

Maternal administration of cannabidiol promotes an anti-inflammatory effect on the intestinal wall in a gastroschisis rat model

Celecoxib ameliorates diabetic neuropathy by decreasing apoptosis and oxidative stress in dorsal root ganglion neurons via the miR‑155/COX‑2 axis

Protective effects of Tat-DJ-1 protein against streptozotocin-induced diabetes in a mice model

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