Influence of nitrate availability on the distribution and abundance of heterotrophic bacterial nitrate assimilation genes in the Barents Sea during summer

Allen, Andrew E.; Booth, Melissa G.; Verity, Peter G.; Frischer, Marc E.

doi:10.3354/ame039247

Cited by 47 publications

(40 citation statements)

References 21 publications

(34 reference statements)

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“…Archaeal amoA assays were run in triplicate by using primers and cycling parameters from Beman et al (48), although run for 40 cycles with the marine group I Crenarchaeota assays similarly adapted from Mincer et al (47). Bacterial 16S rRNA genes were quantified by using primers 932F and 1062R (60). PCR products generated by using the qPCR primers were used as standards in tenfold dilution series from 10 7 to 10 2 copies based on PicoGreen quantification.…”

Section: Methodsmentioning

confidence: 99%

The radiocarbon signature of microorganisms in the mesopelagic ocean

Hansman

Griffin

Watson

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

142

102

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Several lines of evidence indicate that microorganisms in the mesoand bathypelagic ocean are metabolically active and respiring carbon. In addition, growing evidence suggests that archaea are fixing inorganic carbon in this environment. However, direct quantification of the contribution from deep ocean carbon sources to community production in the dark ocean remains a challenge. In this study, carbon flow through the microbial community at 2 depths in the mesopelagic zone of the North Pacific Subtropical Gyre was examined by exploiting the unique radiocarbon signatures (⌬ 14 C) of the 3 major carbon sources in this environment. The radiocarbon content of nucleic acids, a biomarker for viable cells, isolated from size-fractionated particles (0.2-0.5 m and >0.5 m) showed the direct incorporation of carbon delivered by rapidly sinking particles. Most significantly, at the 2 mesopelagic depths examined (670 m and 915 m), carbon derived from in situ autotrophic fixation supported a significant fraction of the free-living microbial community (0.2-0.5 m size fraction), but the contribution of chemoautotrophy varied markedly between the 2 depths. Results further showed that utilization of the ocean's largest reduced carbon reservoir, 14 C-depleted, dissolved organic carbon, was negligible in this environment. This isotopic portrait of carbon assimilation by the in situ, free-living microbial community, integrated over >50,000 L of seawater, implies that recent, photosynthetic carbon is not always the major carbon source supporting microbial community production in the mesopelagic realm.microbial metabolism ͉ particle flux ͉ particulate organic carbon (POC) ͉ chemoautotrophy

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Section: Methodsmentioning

confidence: 99%

The radiocarbon signature of microorganisms in the mesopelagic ocean

Hansman

Griffin

Watson

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

142

102

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“…The availability of nutrients, as the carbon and nitrogen sources, play a major part in the selection of the heterotrophic bacterial community which will be established in an environment (Allen et al, 2005;Davidson et al, 2007). Thus, as analyzed in this study, variations in the composition of the DOM released by P. agardhii during degradation process were coupled changes in the bacterial concentration and composition, with the selection of more or less specialized groups.…”

Section: Dynamics Of Bacterial Communitymentioning

confidence: 92%

“…The quality and availability of compounds in these released DOM and the presence of specialists and generalists bacterial groups will influence the bacterial community composition during the degradation process (Allen et al, 2005;Davidson et al, 2007;Giroldo et al, 2007). Therefore, we focused on trying to estimate the fate of dissolved organic carbon and nitrogen released by axenic cultures of P. agardhii under bacterial degradation, and simultaneously determine the main bacterial OTUs (Operational taxonomic units) involved in the process.…”

Section: Introductionmentioning

confidence: 99%

Bacterial degradation of dissolved organic matter released by Planktothrix agardhii (Cyanobacteria)

et al. 2017

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Although Planktothrix agardhii often produces toxic blooms in eutrophic water bodies around the world, little is known about the fate of the organic matter released by these abundant Cyanobacteria. Thus, this study focused in estimating the bacterial consumption of the DOC and DON (dissolved organic carbon and dissolved organic nitrogen, respectively) produced by axenic P. agardhii cultures and identifying some of the bacterial OTUs (operational taxonomic units) involved in the process. Both P. agardhii and bacterial inocula were sampled from the eutrophic Barra Bonita Reservoir (SP, Brazil). Two distinct carbon degradation phases were observed: during the first three days, higher degradation coefficients were calculated, which were followed by a slower degradation phase. The maximum value observed for particulate bacterial carbon (POC) was 11.9 mg L -1 , which consisted of 62.5% of the total available DOC, and its mineralization coefficient was 0.477 day -1 (t½ = 1.45 days). A similar pattern of degradation was observed for DON, although the coefficients were slightly different. Changes in the OTUs patterns were observed during the different steps of the degradation. The main OTUs were related to the classes Alphaproteobacteria (8 OTUs), Betaproteobacteria (2 OTUs) and Gammaproteobacteria (3 OTUs). The genus Acinetobacter was the only identified organism that occurred during the whole process. Bacterial richness was higher at the slower degradation phase, which could be related to the small amounts of DOM (dissolved organic matter) available, particularly carbon. The kinetics of the bacterial degradation of P. agardhii-originated DOM suggests minimal loss of DOM from the Barra Bonita reservoir.Keywords: dissolved organic matter, bacterial community, DGGE, degradation. Degradação bacteriana da matéria orgânica dissolvida liberada porPlanktothrix agardhii (Cyanobacteria) ResumoEmbora Planktothrix agardhii frequentemente forme florações tóxicas em corpos d'água pelo mundo, pouco ainda se sabe sobre o destino da matéria orgânica liberada por essa abundante Cyanobacteria. Assim, este estudo foi focado na estimativa do consumo bacteriano do carbono orgânico dissolvido (DOC) e nitrogênio orgânico dissolvido (DON) produzido por culturas axênicas de P. agardhii e identificação de algumas das unidades taxonômicas operacionais (OTUs) bacterianas envolvidas no processo. Ambos a linhagem de P. agardhii e o inóculo bacteriano foram amostrados do reservatório eutrófico de Barra Bonita (SP, Brasil). Foram observadas duas fases distintas da degradação do DOC: durante os três primeiros dias, coeficientes mais altos de degradação foram calculados, que foram então seguidos por uma fase mais lenta da degradação do carbono. O valor máximo calculado para o carbono bacteriano particulado (POC) foi de 11,9 mgL -1 , o que equivale a aproximadamente 62,5% do DOC disponível para consumo, e o seu coeficiente de mineralização foi de 0,477 dia -1 (t 1/2 = 1,45 dias). Um padrão similar de degradação foi observado para DON, embora os c...

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“…We found that Marinobacter were dominant in the deep water of the Indian Ocean, with the highest proportion being 42 % of the total sequences. Previous studies have found that the Marinobacter group is of significance for nitrate assimilation and is positively correlated to nitrate concentration [9]. Marinobacter strains have been applied to successfully treat reservoir souring [20].…”

Section: Vertical Distribution Of Nab In the Indian Oceanmentioning

confidence: 99%

“…Inorganic nitrogen, mainly nitrate, is discharged to the coastal water and triggers a series of environmental events. The uptake of nitrate by marine bacteria has important implications for the global nitrogen and carbon balance [9,10]. Nitrogen-related functional microbial communities can potentially consume a substantial fraction of dissolved inorganic nitrogen [11].…”

Section: Introductionmentioning

confidence: 99%

Vertical Distribution of Bacterial Communities in the Indian Ocean as Revealed by Analyses of 16S rRNA and nasA Genes

Jiang

Jiao

2016

Indian J Microbiol

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Bacteria play an important role in the marine biogeochemical cycles. However, research on the bacterial community structure of the Indian Ocean is scarce, particularly within the vertical dimension. In this study, we investigated the bacterial diversity of the pelagic, mesopelagic and bathypelagic zones of the southwestern Indian Ocean (50.46°E, 37.71°S). The clone libraries constructed by 16S rRNA gene sequence revealed that most phylotypes retrieved from the Indian Ocean were highly divergent from those retrieved from other oceans. Vertical differences were observed based on the analysis of natural bacterial community populations derived from the 16S rRNA gene sequences. Based on the analysis of the nasA gene sequences from GenBank database, a pair of general primers was developed and used to amplify the bacterial nitrate-assimilating populations. Environmental factors play an important role in mediating the bacterial communities in the Indian Ocean revealed by canonical correlation analysis.

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Influence of nitrate availability on the distribution and abundance of heterotrophic bacterial nitrate assimilation genes in the Barents Sea during summer

Cited by 47 publications

References 21 publications

The radiocarbon signature of microorganisms in the mesopelagic ocean

The radiocarbon signature of microorganisms in the mesopelagic ocean

Bacterial degradation of dissolved organic matter released by Planktothrix agardhii (Cyanobacteria)

Vertical Distribution of Bacterial Communities in the Indian Ocean as Revealed by Analyses of 16S rRNA and nasA Genes

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