Influence of neuraminidase and mitomycin C on the immunogenicity of Novikoff tumor cells

Significance of glycosylation patterns of rat Zajdela ascitic hepatocellular carcinoma: Effect of enzymatic removal of surface sialic acid on humoral response

“…Further, there was a preferential lysis of NMase-treated cells, which would support the contention that removal of sialic acid residues results in the exposure of tumor-associated antigens of the cell surface, which could induce antibody response. Our observation on the increased antigenicity of NMase-treated ZAH cells is in complete contrast with the studies in Novikoff HCC cells (where there was no difference in the immunogenicity of untreated or NMase-treated cells 36 ) and in rat Walker 256 carcinosarcoma cells (where a decrease in immunogenicity was observed consequent to NMase treatment. 35 ) In the present study, even though we have established that the major loss of sialic acid upon NMase treatment is from O-linked oligosaccharides, it is difficult to delineate specific contributions of N-and O-linked oligosaccharides in determining the properties of ZAH cells.…”

Lectin affinity chromatography of cell surface proteins of novikoff tumor cells

“…For example, Nicolson [30] reported that neuraminidase treatment of erythrocytes, lymphoma cells, and fibroblasts was acconipanied by an increase in RCAI-induced cytoagglutination and binding of labeled lectin to the cell surface. Previous studies in this laboratory demonstrated that the immunogenicity of Novikoff tumor cells was not affected by neuraminidase [32], even though this treatment Binding of almost all major iodinated proteins t o one or more of the Sepharose-con-…”