Influence of n-alkanes and petroleum on fatty acid composition of a hydrocarbonoclastic bacterium: Marinobacter hydrocarbonoclasticus strain 617

Doumenq, Pierre; Aries, Eric; Asia, Laurence; Acquaviva, Monique; Artaud, Jacques; Gilewicz, M.; Mille, G.; Bertrand, Jean-Claude

doi:10.1016/s0045-6535(00)00521-x

Cited by 39 publications

(35 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The correlation between the incorporation of odd-numbered fatty acids into the membrane and the use of odd-numbered n-alkane substrates was reported also for the sedimentary marine bacterium Marinobacter hydrocarbonoclasticus (45). Hence, it can be stated that A. borkumensis is capable of incorporating fatty acids obtained by n-alkane oxidation into their corresponding fatty acids.…”

Section: Discussionmentioning

confidence: 76%

Adaptation of the Hydrocarbonoclastic Bacterium Alcanivorax borkumensis SK2 to Alkanes and Toxic Organic Compounds: a Physiological and Transcriptomic Approach

Naether

Slawtschew

Stasik

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However, investigations of growth behavior and physiology of these bacteria when cultivated with n-alkanes of different chain lengths (C 6 to C 30 ) as the substrates are still lacking. Growth rates increased with increasing alkane chain length up to a maximum between C 12 and C 19 , with no evident difference between even-and odd-numbered chain lengths, before decreasing with chain lengths greater than C 19 . Surface hydrophobicity of alkane-grown cells, assessed by determination of the water contact angles, showed a similar pattern, with maximum values associated with growth rates on alkanes with chain lengths between C 11 and C 19 and significantly lower values for cells grown on pyruvate. A. borkumensis was found to incorporate and modify the fatty acid intermediates generated by the corresponding n-alkane degradation pathway. Cells grown on distinct n-alkanes proved that A. borkumensis is able to not only incorporate but also modify fatty acid intermediates derived from the alkane degradation pathway. Comparing cells grown on pyruvate with those cultivated on hexadecane in terms of their tolerance toward two groups of toxic organic compounds, chlorophenols and alkanols, representing intensely studied organic compounds, revealed similar tolerances toward chlorophenols, whereas the toxicities of different n-alkanols were significantly reduced when hexadecane was used as a carbon source. As one adaptive mechanism of A. borkumensis to these toxic organic solvents, the activity of cis-trans isomerization of unsaturated fatty acids was proven. These findings could be verified by a detailed transcriptomic comparison between cultures grown on hexadecane and pyruvate and including solvent stress caused by the addition of 1-octanol as the most toxic intermediate of n-alkane degradation.

show abstract

Section: Discussionmentioning

confidence: 76%

Adaptation of the Hydrocarbonoclastic Bacterium Alcanivorax borkumensis SK2 to Alkanes and Toxic Organic Compounds: a Physiological and Transcriptomic Approach

Naether

Slawtschew

Stasik

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Upon further investigations into the metabolism of alkanes by Hxd3, Aeckersberg et al observed a clear impact of the chain lengths of the alkane substrates on the cellular fatty acid composition of the bacterium (3). This observation suggests that the strain anaerobically oxidizes alkanes to fatty acids and that it incorporates them into cellular lipids, a metabolic process observed in many aerobic alkane-degrading organisms (5,12,17,21). The relationship between the carbon numbers of alkane substrates and predominant cellular fatty acids, however, was unusual; namely, a C-odd alkane yielded predominantly C-even fatty acids, and a C-even alkane yielded C-odd fatty acids.…”

Section: Discussionmentioning

confidence: 94%

“…Aeckersberg et al reported on the first well-described alkane-degrading, sulfatereducing bacterium, strain Hxd3 (DSM 6200; German Collection of Microorganisms and Cell Cultures), from an oil production plant (1,2). This strain is able to grow anaerobically on C 12 to C 20 alkanes and completely oxidize them to carbon dioxide by coupling to sulfate reduction (1,2). The phenotypic and phylogenetic characteristics of the bacterium indicate that it belongs to the proposed family Desulfobacteriaceae and is closely related to the genus Desulfococcus (1,3).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Anaerobic Transformation of Alkanes to Fatty Acids by a Sulfate-Reducing Bacterium, Strain Hxd3

Phelps

Young

2003

Appl Environ Microbiol

162

106

View full text Add to dashboard Cite

Strain Hxd3, an alkane-degrading sulfate reducer previously isolated and described by Aeckersberg et al. (F. Aeckersberg, F. Bak, and F. Widdel, Arch. Microbiol. 156:5-14, 1991), was studied for its alkane degradation mechanism by using deuterium and 13 C-labeled compounds. Deuterated fatty acids with even numbers of C atoms (C-even) and 13 C-labeled fatty acids with odd numbers of C atoms (C-odd) were recovered from cultures of Hxd3 grown on perdeuterated pentadecane and [1,2-13 C 2 ]hexadecane, respectively, underscoring evidence that C-odd alkanes are transformed to C-even fatty acids and vice versa. When Hxd3 was grown on unlabeled hexadecane in the presence of [ 13 C]bicarbonate, the resulting 15:0 fatty acid, which was one carbon shorter than the alkane, incorporated a 13 C label to form its carboxyl group. The same results were observed when tetradecane, pentadecane, and perdeuterated pentadecane were used as the substrates. These observations indicate that the initial attack of alkanes includes both carboxylation with inorganic bicarbonate and the removal of two carbon atoms from the alkane chain terminus, resulting in a fatty acid one carbon shorter than the original alkane. The removal of two terminal carbon atoms is further evidenced by the observation that the [1,2-13 C 2 ]hexadecane-derived fatty acids contained either two 13 C labels located exclusively at their acyl chain termini or none at all. Furthermore, when perdeuterated pentadecane was used as the substrate, the 14:0 and 16:0 fatty acids formed both carried the same numbers of deuterium labels, while the latter was not deuterated at its carboxyl end. These observations provide further evidence that the 14:0 fatty acid was initially formed from perdeuterated pentadecane, while the 16:0 fatty acid was produced after chain elongation of the former fatty acid with nondeuterated carbon atoms. We propose that strain Hxd3 anaerobically transforms an alkane to a fatty acid through a mechanism which includes subterminal carboxylation at the C-3 position of the alkane and elimination of the two adjacent terminal carbon atoms.

show abstract

“…However, this finding cannot be generalised, because previous studies showed that different bacterial isolates can utilise different long chain n-alkanes under aerobic or anaerobic conditions. [42] For example, under aerobic conditions Marinobacter hydrocarbonoclasticus strain 617 exhibited higher degradation of the C 19 -C 21 than C 16 n-alkanes, [64] whereas under anaerobic conditions sulfate-reducing strains Hxd3 and Pnd3 grew on n-alkanes in the range of C 12 -C 20 and C 14 -C 17 respectively. [65] Moreover, a slurry microcosm study showed that lower molecular weight n-alkanes (C 11 to C 13 ) were more recalcitrant than mid-to high-molecular weight n-alkanes under nitrate-reducing conditions, whereas under sulfate-reducing conditions degradation of long-chain (C 32 to C 39 ) n-alkanes was more extensive.…”

Section: Degradation Of Hexadecane and Kerogen Under Arsenic-reducingmentioning

confidence: 99%

Microbially mediated reduction of FeIII and AsV in Cambodian sediments amended with 13C-labelled hexadecane and kerogen

et al. 2014

View full text Add to dashboard Cite

Environmental context. The use of groundwater with elevated concentrations of arsenic for drinking, cooking or irrigation has resulted in the worst mass poisoning in human history. This study shows that organic compounds that can be found in arsenic rich subsurface sediments may be used by indigenous microorganisms, contributing to the release of arsenic from the sediments into the groundwater. This study increases our understanding of the range of organic substrates (and their sources) that can potentially stimulate arsenic mobilisation into groundwaters.Abstract. Microbial activity is generally accepted to play a critical role, with the aid of suitable organic carbon substrates, in the mobilisation of arsenic from sediments into shallow reducing groundwaters. The nature of the organic matter in natural aquifers driving the reduction of As V to As III is of particular importance but is poorly understood. In this study, sediments from an arsenic rich aquifer in Cambodia were amended with two 13 C-labelled organic substrates. 13C-hexadecane was used as a model for potentially bioavailable long chain n-alkanes and a 13 C-kerogen analogue as a proxy for non-extractable organic matter. During anaerobic incubation for 8 weeks, significant Fe III reduction and As III mobilisation were observed in the biotic microcosms only, suggesting that these processes were microbially driven. Microcosms amended with 13 C-hexadecane exhibited a similar extent of Fe III reduction to the non-amended microcosms, but marginally higher As III release. Moreover, gas chromatography-mass spectrometry analysis showed that 65 % of the added 13 C-hexadecane was degraded during the 8-week incubation. The degradation of 13 C-hexadecane was microbially driven, as confirmed by DNA stable isotope probing (DNA-SIP). Amendment with 13 C-kerogen did not enhance Fe III reduction or As III mobilisation, and microbial degradation of kerogen could not be confirmed conclusively by DNA-SIP fractionation or 13 C incorporation in the phospholipid fatty acids. These data are, therefore, consistent with the utilisation of long chain n-alkanes (but not kerogen) as electron donors for anaerobic processes, potentially including Fe III and As V reduction in the subsurface.

show abstract

Influence of n-alkanes and petroleum on fatty acid composition of a hydrocarbonoclastic bacterium: Marinobacter hydrocarbonoclasticus strain 617

Cited by 39 publications

References 29 publications

Adaptation of the Hydrocarbonoclastic Bacterium Alcanivorax borkumensis SK2 to Alkanes and Toxic Organic Compounds: a Physiological and Transcriptomic Approach

Adaptation of the Hydrocarbonoclastic Bacterium Alcanivorax borkumensis SK2 to Alkanes and Toxic Organic Compounds: a Physiological and Transcriptomic Approach

Anaerobic Transformation of Alkanes to Fatty Acids by a Sulfate-Reducing Bacterium, Strain Hxd3

Microbially mediated reduction of FeIII and AsV in Cambodian sediments amended with 13C-labelled hexadecane and kerogen

Contact Info

Product

Resources

About