Influence of N-acetylcysteine on the formation of biofilm by Staphylococcus epidermidis

Pérez-Giraldo, Ciro; Rodríguez-Benito, A.; Morán, F. J.; Hurtado, C.; Blanco, M. T.; Gómez-García, A. C.

doi:10.1093/jac/39.5.643

Cited by 146 publications

(104 citation statements)

References 9 publications

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“…NAC has been used in med- icine for the treatment of cystic fibrosis (25,42,43), but the effects of NAC on bacterial biofilm processes have not been investigated in depth. NAC is considered to be a nonantibiotic drug but to have antibacterial (bacteriostatic) properties (31,32). NAC did indeed reduce growth to various degrees for all strains tested, as well as the multispecies community (Fig.…”

Section: Discussionmentioning

confidence: 94%

“…Despite its importance as a medicine, the effect of NAC on bacteria has been poorly studied. Perez-Giraldo et al (32) showed that NAC reduced the formation of biofilms of Staphylococcus epidermidis on a polystyrene surface but did not distinguish between the effects on bacteria and bacterial EPS. Recently, it was shown that NAC reduced adhesion of Streptococcus pneumoniae and Haemophilus influenzae to oropharyngeal epithelial cells in vitro (36).…”

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confidence: 99%

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N -Acetyl- l -Cysteine Affects Growth, Extracellular Polysaccharide Production, and Bacterial Biofilm Formation on Solid Surfaces

Olofsson¹,

Hermansson

Elwing³

2003

Appl Environ Microbiol

214

192

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N-Acetyl-L-cysteine (NAC) is used in medical treatment of patients with chronic bronchitis. The positive effects of NAC treatment have primarily been attributed to the mucus-dissolving properties of NAC, as well as its ability to decrease biofilm formation, which reduces bacterial infections. Our results suggest that NAC also may be an interesting candidate for use as an agent to reduce and prevent biofilm formation on stainless steel surfaces in environments typical of paper mill plants. Using 10 different bacterial strains isolated from a paper mill, we found that the mode of action of NAC is chemical, as well as biological, in the case of bacterial adhesion to stainless steel surfaces. The initial adhesion of bacteria is dependent on the wettability of the substratum. NAC was shown to bind to stainless steel, increasing the wettability of the surface. Moreover, NAC decreased bacterial adhesion and even detached bacteria that were adhering to stainless steel surfaces. Growth of various bacteria, as monocultures or in a multispecies community, was inhibited at different concentrations of NAC. We also found that there was no detectable degradation of extracellular polysaccharides (EPS) by NAC, indicating that NAC reduced the production of EPS, in most bacteria tested, even at concentrations at which growth was not affected. Altogether, the presence of NAC changes the texture of the biofilm formed and makes NAC an interesting candidate for use as a general inhibitor of formation of bacterial biofilms on stainless steel surfaces.

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Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

N -Acetyl- l -Cysteine Affects Growth, Extracellular Polysaccharide Production, and Bacterial Biofilm Formation on Solid Surfaces

Olofsson¹,

Hermansson

Elwing³

2003

Appl Environ Microbiol

214

192

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“…Staphylococcal species, together with a high bacterial burden, has been associated with reduced survival in IPF (2,4). NAC has been shown to reduce biofilm formation and enhance intracellular killing of Staphylococcus (37,38), which may further enhance airway host defense in the presence of the rs35705950 polymorphism.…”

Section: Original Articlementioning

confidence: 99%

TOLLIP, MUC5B, and the Response to N-Acetylcysteine among Individuals with Idiopathic Pulmonary Fibrosis

Oldham

Fan

Martínez

et al. 2015

Am J Respir Crit Care Med

273

191

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Rationale: Idiopathic pulmonary fibrosis (IPF) is a devastating lung disease of unknown etiology. The genes TOLLIP and MUC5B play important roles in lung host defense, which is an immune process influenced by oxidative signaling. Whether polymorphisms in TOLLIP and MUC5B modify the effect of immunosuppressive and antioxidant therapy in individuals with IPF is unknown.Objectives: To determine whether single-nucleotide polymorphisms (SNPs) within TOLLIP and MUC5B modify the effect of interventions in subjects participating in the Evaluating the Effectiveness of Prednisone, Azathioprine, and N-Acetylcysteine in Patients with Idiopathic Pulmonary Fibrosis (PANTHER-IPF) clinical trial.Methods: SNPs within TOLLIP (rs5743890/rs5743894/rs5743854/ rs3750920) and MUC5B (rs35705950) were genotyped. Interaction modeling was conducted with multivariable Cox regression followed by genotype-stratified survival analysis using a composite endpoint of death, transplantation, hospitalization, or a decline of >10% in FVC.Measurements and Main Results: Significant interaction was observed between N-acetylcysteine (NAC) therapy and rs3750920 within TOLLIP (P interaction = 0.001). After stratifying by rs3750920 genotype, NAC therapy was associated with a significant reduction in composite endpoint risk (hazard ratio, 0.14; 95% confidence interval, 0.02-0.83; P = 0.03) in those with a TT genotype, but a nonsignificant increase in composite endpoint risk (hazard ratio, 3.23; 95% confidence interval, 0.79-13.16; P = 0.10) was seen in those with a CC genotype. These findings were then replicated in an independent IPF cohort.Conclusions: NAC may be an efficacious therapy for individuals with IPF with an rs3750920 (TOLLIP) TT genotype, but it was associated with a trend toward harm in those with a CC genotype. A genotype-stratified prospective clinical trial should be conducted before any recommendation regarding the use of off-label NAC to treat IPF.

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“…The result was expressed as a percentage relative to the control without fermented sumach. For this purpose, the following formula was applied: SI=100 x (mean density of biofilm with supplement/mean growth with treatment)/(mean density of biofilm without treatment/mean growth without treatment) (Pérez-Giraldo C. et al, 1997) (23).…”

Section: Methodsmentioning

confidence: 99%

Effects of Fermented Sumach on the Formation of Slime Layer of Staphylococcus aureus

Kırmusaoğlu¹,

Yurdugül²,

Koçoğlu³

2012

Balkan Med J

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Objective: Staphylococcus aureus (S. aureus) is one of the most commonly isolated bacterial pathogens in hospitals, and the most frequent cause of nosocomial infections. Nosocomial staphylococcal foreign-body infections related to biofilm formation are a serious threat, demanding new therapeutic and preventive strategies. Implantation of intravenous catheters and surgical implantation of prosthetic implants carry a risk of infection. In order to prevent all these effects of biofilms, a study was designed to observe the possible antibacterial effect of sumach (Rhus coriaria) on the biofilm formation of S. aureus. Material and Methods:The influence of varying concentrations of sumach on the formation of biofilms by 13 strains of Staphylococcus aureus was tested by a microelisa assay.Results: The significant differences between varying concentrations of sumach (0.1, 0.2, 0.5 and 1.0 µl/ml) were observed in four methicillin resistant Staphylococcus aureus (MRSA) and nine methicillin sensitive Staphylococcus aureus (MSSA) (p<0.05). In bacteria, a dose-related decrease in the formation of slime, which is a major virulence factor of staphylococcal infections, was observed.Conclusion: In our study, using 0.1, 0.2, 0.5 and 1.0 µl/ml of sumach, thirteen strains lost, 17%, 22%, 28% and 48% respectively of their capacity to produce biofilms. Sumach, which is a herbal product, can decrease the formation of biofilm, which is a major virulence factor in staphylococcal infections.

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Influence of N-acetylcysteine on the formation of biofilm by Staphylococcus epidermidis

Cited by 146 publications

References 9 publications

N -Acetyl- l -Cysteine Affects Growth, Extracellular Polysaccharide Production, and Bacterial Biofilm Formation on Solid Surfaces

N -Acetyl- l -Cysteine Affects Growth, Extracellular Polysaccharide Production, and Bacterial Biofilm Formation on Solid Surfaces

TOLLIP, MUC5B, and the Response to N-Acetylcysteine among Individuals with Idiopathic Pulmonary Fibrosis

Effects of Fermented Sumach on the Formation of Slime Layer of Staphylococcus aureus

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