Influence of MLS laser radiation on erythrocyte membrane fluidity and secondary structure of human serum albumin

Abstract

The biostimulating activity of low level laser radiation of various wavelengths and energy doses is widely documented in the literature, but the mechanisms of the intracellular reactions involved are not precisely known. The aim of this paper is to evaluate the influence of low level laser radiation from an multiwave locked system (MLS) of two wavelengths (wavelength = 808 nm in continuous emission and 905 nm in pulsed emission) on the human erythrocyte membrane and on the secondary structure of human serum albumin (HSA). Human erythrocytes membranes and HSA were irradiated with laser light of low intensity with surface energy density ranging from 0.46 to 4.9 J cm−2 and surface energy power density 195 mW cm−2 (1,000 Hz) and 230 mW cm−2 (2,000 Hz). Structural and functional changes in the erythrocyte membrane were characterized by its fluidity, while changes in the protein were monitored by its secondary structure. Dose-dependent changes in erythrocyte membrane fluidity were induced by near-infrared laser radiation. Slight changes in the secondary structure of HSA were also noted. MLS laser radiation influences the structure and function of the human erythrocyte membrane resulting in a change in fluidity.

Influence of high-energy laser therapy to the patellar tendon on its ligamentous microcirculation: a quasi-experiment

“…In laboratory studies, there is some evidence of changes in erythrocytes and platelets due to laser therapy that significantly affect flow characteristics. Thereby, the blood viscosity decreases and certain stress adaptation of erythrocytes occurs [9–11]. The phenomenon of an apparent decrease in relative hemoglobin observed in the study would also be consistent with this.…”

“…In addition, the lifespan of erythrocytes and platelets is prolonged and their flow properties are improved under HILT [9][10][11]. Therefore, HILT is also discussed as a promising method for the treatment of various blood diseases [9][10][11][12][13].…”

“…HILT decreases erythrocyte deformability and platelet aggregation, resulting in membrane revitalization, viscosity reduction, and erythrocyte stress adaptation. In addition, the lifespan of erythrocytes and platelets is prolonged and their flow properties are improved under HILT [9][10][11]. Therefore, HILT is also discussed as a promising method for the treatment of various blood diseases [9][10][11][12][13].…”

Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer

“…MTSS1 expression was higher in normal gastric mucosa but decreased or absent in gastric cancer tissues, suggesting a correlation with a high frequency of LOH in the 5' promoter sequence (37,45). The same results were also reported about LOH in cancer of the gastroesophageal junction (46), cervical carcinoma (47) and in other progressed tumors which include transcriptional suppression by DNMT3B (48) or microRNA-mediated effects (49)(50)(51)(52)(53)(54).…”

Low-level light therapy reduces platelet destruction during extracorporeal circulation

“…Other studies 7 , 8 have reported that R/NIR radiation increases the electrochemical potential of erythrocytes, which may directly contribute to the decrease of their aggregation potential during rouleaux formation 9 . In addition, modulation of membrane enzyme activity has been repeatedly demonstrated 10 , 11 . Moreover, RBC membrane lipid peroxidation in response to ozonation was reduced in the presence of NIR irradiation 8 .…”