Influence of miR-7a and miR-24-3p on the SOX18 transcript in lung adenocarcinoma

Olbromski, Mateusz; Rzechonek, Adam; Grzegrzółka, Jędrzej; Glatzel-Plucińska, Natalia; Chachaj, Angelika; Weryńska, Bożena; Podhorska-Okołów, Marzena; Dzięgiel, Piotr

doi:10.3892/or.2017.6077

Cited by 15 publications

(14 citation statements)

References 53 publications

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“…It has previously been found that miRNA expression is altered in various tumors cells. 11 – 13 miR-1269 was found to be overexpressed in colorectal, liver and esophagus cancers and to further regulate cell proliferation, apoptosis, epithelial–mesenchymal transition (EMT), and metastasis. 14 – 17 It is believed that miR-1269 could be a potentially prognostic biomarker and therapeutic target, however the precise role of miR-1269 in lung cancer is as yet unknown.…”

Section: Introductionmentioning

confidence: 99%

miR-1269 promotes cell survival and proliferation by targeting tp53 and caspase-9 in lung cancer

Bao¹,

Song²,

Xia³

et al. 2018

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Background and aimLung cancer is the leading cause of cancer death worldwide. In this study, we aim to elucidate the role of miR-1269 in the pathogenesis of lung cancer.Methods and resultsFrom the results of analyses using The Cancer Genome Atlas (TCGA) database, we noted the expression of miR-1269 was increased in lung cancer tissue. miR-1269 expression was detected in both the normal adjacent lung tissue and in the tumorous lung tissue of lung cancer patients, and miR-1269 was more highly expressed in the tumors. High expression of miR-1269 correlated with patients’ tumor stage and lymph node metastasis. A Cell Counting Kit-8 (CCK8) analysis and a cloning formation assay showed that overexpression of miR-1269 significantly promoted the growth of A549 cells, and that a lower expression of miR-1269 significantly increased cell apoptosis. We used the TargetScan 6.2 Database to predict the potential targets of miR-1269, and a luciferase activity assay was used to determine the direct interaction between miR-1269, tumor protein p53 (TP53), and caspase-9. Results from Western blots and real-time PCR showed that overexpression of miR-1269 significantly inhibited TP53 and caspase-9 expression. In addition, caspase-3 activity was found to decrease in a miR-1269 mimic group. The results showed that gene silencing of TP53 and caspase-9 significantly inhibited A549 cell growth and promoted cell apoptosis. The results also showed that the inhibition of miR-1269 and caspase-9 expression inhibited cell apoptosis. Immunohistochemistry (IHC) results demonstrated that TP53 and caspase-9 were expressed in low levels in tumor tissues, and that an inverse correlation exists between miR-1269 expression levels and TP53 or caspase-9 expression levels.ConclusionThese results demonstrate that miR-1269 promotes cell survival and proliferation by targeting TP53 and caspase-9 in lung cancer.

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Section: Introductionmentioning

confidence: 99%

miR-1269 promotes cell survival and proliferation by targeting tp53 and caspase-9 in lung cancer

Bao¹,

Song²,

Xia³

et al. 2018

OTT

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“…Later experiments revealed the participation of two miRNA molecules in this phenomenon: miR-7a and miR-24-3p (9). The results of the analysis of the level of these molecules in cases of lung adenocarcinoma (AC) and squamous-cell lung carcinoma (SQC) and their corresponding cases of NMLT point to a mechanism carried out by the cancer cells through which they reduce the primary high level of the miRNA molecules found in normal lung tissue (9,52). Therefore, the demonstration of the role of specific miRNAs and the role of the methylation of the SOX18 gene promoter in the above-described mechanism may be used in the future in diagnostics, prognostic assessment and, possibly, in NSCLC targeted therapy as well.…”

Section: Non-small Cell Lung Cancermentioning

confidence: 99%

Role of the SOX18 protein in neoplastic processes (Review)

2018

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Abstract. There is a high demand for anticancer drugs due to the fact that the chemotherapeutics currently used have numerous side effects, which lowers the patient's quality of life. However, the latest antibody therapies are extremely expensive, hence the requirement to identify novel, equally effective but low-toxic treatments that have limited side effects. As a result of this, a number of research centres around the world are attempting to identify novel molecular markers that could be effective targets for anticancer therapy in the future. The SOX18 protein has been suggested to be a significant diagnostic and prognostic marker in various types of cancer. SRY-related HMG-box 18 (SOX18) is an important transcription factor involved in the development of cardiovascular and lymphatic vessels during embryonic development. In addition, it is involved in the progression of atherosclerosis and wound-healing processes. It has been observed that its level is higher in a number of cancer types, including melanoma, pancreas, stomach, liver, breast, lung, ovarian and cervical cancer. Furthermore, an association between a high expression of SOX18 in gastric cancer stromal cells and a poor prognosis has been demonstrated. The literature indicates how complex the pathogenesis of cancer is. Knowing the molecular basis of the pathogenesis of the tumor will allow for the effective use of targeted therapy, which may result in a higher success in treating patients. It is therefore important to identify novel and effective therapies as well as new proteins that could be potential markers. The SOX18 family, represented by the SOX18 protein, seems to be in this respect a promising element in modern anticancer therapy.

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“…On the one hand, compared with normal tissues, miR-24 was found to be up-regulated in the following human cancers: lung cancer (LC) ( 6 , 44 , 45 ), hepatocellular carcinoma (HCC) ( 7 ), breast cancer (BC) ( 8 ), tongue squamous cell carcinoma (TSCC) ( 42 ), bladder cancer (BLC) ( 46 ), gastric cancer (GC) ( 47 ), acute leukemia (AL) ( 48 ), and Hodgkin Lymphoma (HL) ( 49 ). On the other hand, miR-24 was found to be down-regulated in the following human cancers: nasopharyngeal carcinoma (NPC) ( 9 ), colorectal cancer (CRC) ( 10 ), laryngeal squamous cell carcinoma (LSCC) ( 11 ), prostate cancer (PC) ( 27 , 28 ), lung adenocarcinoma (LA) ( 29 , 30 ), bladder cancer (BLC) ( 31 ), and gastric cancer (GC) ( 32 ), compared to the normal tissues. Specifically, miR-24 promoted lung cancer progression by regulating the tumor suppressor gene menin ( 45 ).…”

Section: Therapeutic Relevance Of Mir-24 In Cancermentioning

confidence: 99%

MicroRNA-24 in Cancer: A Double Side Medal With Opposite Properties

et al. 2020

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MicroRNA-24 (miR-24) has been widely studied in a variety of human cancers, which plays different roles in specific type of cancers. In the present review, we summarized the recent surveys regarding the role of miR-24 in different human cancers. On the one hand, miR-24 was reported to be down-regulated in some types of cancer, indicating its role as a tumor suppressor. On the other hand, it has shown that miR-24 was up-regulated in some other types of cancer, even in the same type of cancer, suggesting the role of miR-24 being as an oncogene. Firstly, miR-24 was dysregualted in human cancers, which is related to the clinical performance of cancer patients. Thus miR-24 could be used as a potential non-invasive diagnostic marker in human cancers. Secondly, miR-24 was associated with the tumor initiation and progression, being as a promoter or inhibitor. Therefore, miR-24 might be an effective prognostic biomarker in different type of cancers. Lastly, the abnormal expression of miR-24 was involved in the chemo- and radio- therapies of cancer patients, indicating the role of miR-24 being as a predictive biomarker to cancer treatment. Totally, miR-24 contributes to tumorigenesis, tumor progression, and tumor therapy, which closely related to clinic. The present review shows that miR-24 plays a double role in human cancers and provides plenty of evidences to apply miR-24 as a potential novel therapeutic target in treating human cancers.

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Influence of miR-7a and miR-24-3p on the SOX18 transcript in lung adenocarcinoma

Cited by 15 publications

References 53 publications

miR-1269 promotes cell survival and proliferation by targeting tp53 and caspase-9 in lung cancer

miR-1269 promotes cell survival and proliferation by targeting tp53 and caspase-9 in lung cancer

Role of the SOX18 protein in neoplastic processes (Review)

MicroRNA-24 in Cancer: A Double Side Medal With Opposite Properties

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