Influence of minerals on lead-induced alterations in liver function in rats exposed to long-term lead exposure

Herman, D'souza Sunil; Menezes, Geraldine; Venkatesh, Talavara

doi:10.1016/j.jhazmat.2008.12.070

Cited by 32 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The liver is considered as one of the target organs affected by lead toxicity owing to its storage, biotransformation and detoxification in the liver after lead exposure is of relevance in heavy metal poisoning (Herman et al, 2009). The tissues of the liver across all groups in the experimental stages were observed to have liver with focal periportal vascular congestion and hypertrophy, moderate chronic inflammatory cell infiltration and interstitial oedema as shown in Plate 3.…”

Section: Discussionmentioning

confidence: 94%

Ameliorative Effect of Different Concentration of Mushroom (Pleurotus Tuberregium) on Pathomorphological Changes Induced By Lead Toxicity in Liver and Kidney of Wistar Albino Rats

Enagbonma¹,

Tawari-fufenyi²,

Ekaye³

et al. 2015

Nig. J. Biotechnol

View full text Add to dashboard Cite

This study evaluates ameliorative effect of different concentrations of mushroom (Pleurotus tuberregium) on the effects of lead (Pb). Ninety albino rats were divided into six groups with three replicates (5 rats per group) for 21 days pre-experimental stage for acclimatization. The experimental groups were exposed to 0.1 g/l of lead daily for 21 days. At the end of exposure period, lead salt was discontinued for 21 and 42days to verify possible ameliorative effect of mushroom in the post-experimental stage. Samples of liver and kidney tissues were then collected and subsequently analyzed for structural changes every 21 days. The tissues of the pre-experimental stages showed normal tissues as those of the control except in the liver which showed mild periportal chronic inflammatory cell. However, the experimental groups, showed liver with focal periportal vascular congestion and hypertrophy, moderate chronic inflammatory cell infiltration and interstitial oedema. The kidney showed focal proximal tubular necrosis, transmural oedema, interstitial haemorrhage, and chronic inflammatory cell infiltration. The tissues of liver and kidney in the post experimental stages were improved with increased mushroom content in the feed. Results of this study demonstrated that P. tuberregium may ameliorate lead toxicity in liver and kidney tissues.

show abstract

Section: Discussionmentioning

confidence: 94%

Ameliorative Effect of Different Concentration of Mushroom (Pleurotus Tuberregium) on Pathomorphological Changes Induced By Lead Toxicity in Liver and Kidney of Wistar Albino Rats

Enagbonma¹,

Tawari-fufenyi²,

Ekaye³

et al. 2015

Nig. J. Biotechnol

View full text Add to dashboard Cite

show abstract

“…The albumin level in group F (MO+ Pb) was significantly lower compared to group C (MO), D (Pb) and the control group. The effect of lead exposure on the serum protein levels is controversial; Dioka et al (2004), Pachathundikandi et al (2006), andHerman et al (2009) observed no change in serum albumin level in automobile workers and albino rats exposed to long-term moderate lead concentrations. Histological and histochemical results of this study clearly support earlier reports that, lead has nephrotoxic effect on renal tissue and lead poisoning is associated with oxidative stress in blood and brain tissues (Adewole and Ayoka, 2009).…”

Section: Discussionmentioning

confidence: 99%

Microanatomy and histomorphometry analysis of the effects of Moringa oleifera leaf extract on lead-induced kidney damage in adult wistar rats

Olatunji¹,

Adewole²,

Ayannuga³

et al. 2015

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

This study evaluated the effects of lead exposure on the histoarchitecture of kidney, assessed the effects of lead on the levels of creatinine, urea and albumin in the plasma and determined the effects of M. oleifera leaf extract (MOLE) on the histoarchitecture of kidney in Wistar rats after lead-induced kidney damage. This was with a view to providing information on the ameliorative effects of M. oleifera leaf extract on lead-induced kidney damage. Thirty adult male Wistar rats weighing 120 -180 g were used for this study. Kidney damage was experimentally induced by daily administration of lead acetate (50 mg/kg/day) for a period of 14 days. The test groups were treated with the M. oleifera leaf extract (100 mg/kg/day) for 14 days. At the end of treatment period, the rats were sacrificed and their kidneys were excised for histological and histomorphometric studies. Markers of renal function were biochemically determined in the plasma using enzyme calorimetric assay kit. Histomorphological examinations of the stained kidney sections revealed that 100 mg/kg of MOLE had no adverse effects on the kidney of group C rats as the morphology of kidney of the rats in this group were normal and comparable with that of groups A and B. However, deleterious effects such as distortion of Bowman's capsule with diminished glomerular space, structural alteration of proximal and distal convoluted tubules were observed in the kidney of group D rats following lead-induced damage. Treatment with MOLE protected the kidneys of groups E and F rats from lead-induced damage as the renal morphology appeared normal. The results of biochemical analysis revealed a significant increase in the plasma level of urea (F=203.9, p = 0.0001), and creatinine (F= 7.42, p = 0.0002), in group D rats (56.79 ± 0.06 g/l, 2.91 ± 0.07 mg/dl), respectively compared with groups A (14.02±1.53 g/l, 2.33 ± 0.06 mg/dl), B (50.44±1.75 g/l, 2.54 ± 0.06 mg/dl), C (58.91±2.95 g/ l, 2.42 ± 0.11 mg/dl), E (12.18±1.45 g/l, 2.45 ± 0.08 mg/dl) and F (7.48±1.00 g/l, 2.39 ± 0.08 mg/dl) respectively. This is an indication of impaired renal function. Also, the results of the histomorphometry analysis showed a significant decrease in the number of glomeruli present in each photomicrograph and the diameter of the urinary space in group D respectively compared with groups A, B, C, E and F. This study showed that treatment with Moringa oleifera prevented the toxicity brought about by lead exposure and this is evidenced by an enhancement in the glomerular morphology and clearly seen renal tubules. In conclusion, our findings suggest that Moringa oleifera leaf extract had ameliorative and protective properties on lead-induced kidney injury.

show abstract

“…Also, the liver being one of the major organs involved in the storage, biotransformation and detoxification of toxic substances, is of relevance in heavy metal poisoning [28]. Absorbed lead is stored in soft tissues mainly in the liver [29] via the portal vein, so that it is the first organ for which the histological analysis can be used to examine the morphological changes that reflect possible lead effects on somatic cells [30].…”

Section: Discussionmentioning

confidence: 99%

Hepatotoxic Effects of Lead Acetate in Rats: Histopathological and Cytotoxic Studies

Zidi¹

2014

J Cytol Histol

View full text Add to dashboard Cite

Lead toxicity is probably the most common form of heavy metal intoxication. The present study was conducted to assess the histopathological and cytotoxic effects of lead exposure on rat liver.Adult male Wistar rats were randomly divided in 2 groups. The first was exposed to 2 g of lead acetate in distilled water during 35 days, while the second served as a control group and was given distilled water. The structural damage in the liver was investigated by histological study and supplemented by biochemical assay of liver enzyme levels. DNA fragmentation in somatic cells was determined using terminal desoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay.The results obtained show increase in liver enzyme levels in treated rats compared to controls. The histological study showed that lead can induce several alterations such as hypertrophy of hepatocytes, portal space and central vein dilatation, vacuolation and lymphocytic infiltration. Tunel assay revealed significant DNA fragmentation in rats exposed to lead. This study showed that TUNEL assay can be used to determinate DNA fragmentation in somatic cells of rat liver. Moreover, we conclude that lead acetate may be considered as a strong hepatotoxic and genotoxic agent.

show abstract

Influence of minerals on lead-induced alterations in liver function in rats exposed to long-term lead exposure

Cited by 32 publications

References 33 publications

Ameliorative Effect of Different Concentration of Mushroom (<i>Pleurotus Tuberregium</i>) on Pathomorphological Changes Induced By Lead Toxicity in Liver and Kidney of Wistar Albino Rats

Ameliorative Effect of Different Concentration of Mushroom (<i>Pleurotus Tuberregium</i>) on Pathomorphological Changes Induced By Lead Toxicity in Liver and Kidney of Wistar Albino Rats

Microanatomy and histomorphometry analysis of the effects of <i>Moringa oleifera</i> leaf extract on lead-induced kidney damage in adult wistar rats

Hepatotoxic Effects of Lead Acetate in Rats: Histopathological and Cytotoxic Studies

Contact Info

Product

Resources

About