Influence of metformin on GLUT1 gene and protein expression in rat streptozotocin<i>diabetes mellitus</i>model

Sokolovska, Jeļizaveta; Isajevs, Sergejs; Sugoka, Olga; Sharipova, Jelena; Lauberte, Lasma; Svirina, Darja; Rostoka, Evita; Sjakste, Tatjana; Kalvinsh, Ivars; Sjakste, Nikolajs

doi:10.3109/13813455.2010.494672

Cited by 33 publications

(22 citation statements)

References 35 publications

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“…Single dose STZ administration is a very well-documented and an accepted model of diabetes in rats (Leung et al 2010;Sharma and Singh 2010;Sokolovska et al 2010). STZ induced experimental diabetes is widely used for the assessment of diabetic conditions and its secondary complications like diabetic neuropathy (Chopra et al 2010), diabetic nephropathy (Marotta et al 2010), diabetic cardiomyopathy (Huynh et al 2010), cardiac hypertrophy (Feng et al 2010), endothelial dysfunction (Olukman et al 2010), post stroke dementia and tau abnormality (Zhang et al 2010), diabetic retinopathy (Rao et al 2010), diabetic erectile dysfunction (Yohannes et al 2010), etc.…”

Section: Discussionmentioning

confidence: 99%

Attenuation of vascular dementia by sodium butyrate in streptozotocin diabetic rats

Sharma

Singh

2011

Psychopharmacology

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Section: Discussionmentioning

confidence: 99%

Attenuation of vascular dementia by sodium butyrate in streptozotocin diabetic rats

Sharma

Singh

2011

Psychopharmacology

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“…GLUT1 provides cells with glucose, but its abnormal expression also has a relationship with tumor growth and diabetes [9], [10], [34], [35]. We have shown that hypoxia and low sugar can induce GLUT1 expression and that the growth and metabolism of cancer cells expressing GLUT1 is rather higher than in other cells.…”

Section: Discussionmentioning

confidence: 99%

Functional Analyse of GLUT1 and GLUT12 in Glucose Uptake in Goat Mammary Gland Epithelial Cells

Zhu

Lin

et al. 2013

PLoS ONE

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Glucose transport, mediated by glucose transporters, is necessary for mammary gland development and lactation. GLUT1 and GLUT12 could both be expressed in the pregnant and lactating mammary gland to participate in the glucose uptake process. In this study, the goat GLUT1 and GLUT12 genes were cloned from Saanen dairy goats and transfected into goat mammary gland epithelial cells to assess their biological functions and distributions. The results showed that both goat GLUT1 and GLUT12 had 12 predicted membrane-spanning helices. Goat GLUT1 and GLUT12 each influenced the mRNA expression of the other transporter and increased the glucose consumption and lactose yield in GLUT1- and GLUT12-transfected goat mammary gland epithelial cells, respectively. The overexpression of GLUT1 or GLUT12 also increased the expression of amino acid transporters SLC1A5, SLC3A2 and SLC7A5 and affected genes expressions in GMGE cells. Using immunofluorescence staining, GLUT1 was detected throughout the cytoplasm and localized to the Golgi apparatus around the nuclear membrane, whereas GLUT12 was mainly distributed in the perinuclear region and cytoplasm. This study contributes to the understanding of how GLUT1 and GLUT12 cooperate in the incorporation of nutrient uptake into mammary gland epithelial cells and the promotion of milk synthesis in the goat mammary gland during lactation.

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“…In STZ-induced diabetic rats, metformin and 5-aminoimidazole-4-carboxamide-1β-riboside (AICAR) increased renal AMPK phosphorylation, reversed mTOR activation, and inhibited renal hypertrophy (Lee et al 2007). Metformin treatment also improves hyperglycemia via mechanisms that include activation of AMPK (Sokolovska et al 2010). Treatment with resveratrol, another AMPK activator, reversed the inhibition of AMPK in the STZ-induced diabetic kidney and reduced albuminuria, ameliorated hyperglycemia and renal dysfunction, and attenuated renal hypertrophy (Ding et al 2010 a , Chang et al 2011).…”

Section: Ampk and Autophagy In Diabetic Kidneymentioning

confidence: 99%

Autophagy in diabetic nephropathy

Ding¹,

Choi²

2014

Journal of Endocrinology

262

222

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Diabetic nephropathy (DN) is the most common cause of end-stage kidney disease worldwide, and is associated with increased morbidity and mortality in patients with both type 1 and type 2 diabetes. Increasing prevalence of diabetes has made the need for effective treatment of DN critical, and identifying new therapeutic targets to improve clinical management. Autophagy is a highly conserved “self-eating” pathway by which cells degrade and recycle macromolecules and organelles. Autophagy serves as an essential mechanism to maintain homeostasis of glomeruli and tubules, and plays important roles in human health and diseases. Impairment of autophagy is implicated in the pathogenesis of DN. Emerging body of evidence suggests that targeting the autophagic pathway to activate and restore autophagy activity may be renoprotective. Here we review current advances in our understanding of the roles of autophagy in diabetic kidney injury, focusing on studies in renal cells in culture, human kidney tissues, and experimental animal models of diabetes. We discuss the major nutrient-sensing signal pathways, and diabetes-induced altered intracellular metabolism and cellular events, including accumulation of advanced glycation end-products, increased oxidative stress, endoplasmic reticulum stress, hypoxia, and activation of the renin angiotensin system, which modulate autophagic activity and contribute to the development of DN. We also highlight recent studies of autophagy and transforming growth factor-β in renal fibrosis, the final common response to injury that ultimately leads to end-stage kidney failure in both type 1 and type 2 diabetes. These findings suggest the possibility that autophagy can be a therapeutic target against DN.

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Influence of metformin on GLUT1 gene and protein expression in rat streptozotocindiabetes mellitusmodel

Abstract: Metformin is prospective for treatment of diabetic nephropathy.

Cited by 33 publications

References 35 publications

Attenuation of vascular dementia by sodium butyrate in streptozotocin diabetic rats

Attenuation of vascular dementia by sodium butyrate in streptozotocin diabetic rats

Functional Analyse of GLUT1 and GLUT12 in Glucose Uptake in Goat Mammary Gland Epithelial Cells

Autophagy in diabetic nephropathy

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