Influence of metabolism modifiers of cyclic nucleotides on contractility of right ventricle of rat heart with intact and removed endocardial endothelium

Smiljic, Sonja; Radovic, Dragica; Miletic, Milanka; Nestorović, Vojkan; Trajković, Goran; Savic, Sladjana

doi:10.2298/sarh1010577s

Cited by 2 publications

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“…The endocardium is a natural biological barrier between the circulating blood of the ventricles and cardiomyocytes, including endocardial and microvascular endothelial cells. The endocardium is essential for cardiac embryonic development, cardiac contraction, rhythm, and remodeling ( Brutsaert, 2003 ; Smiljić et al, 2010 ). Endocardial injury reduces endothelial-mediated NO release and cardiomyocyte sensitivity to Ca 2+ and contractility ( Shen et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Single-nucleus ribonucleic acid-sequencing and spatial transcriptomics reveal the cardioprotection of Shexiang Baoxin Pill (SBP) in mice with myocardial ischemia-reperfusion injury

et al. 2023

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Graphical AbstractSingle-cell landscape of SBP treatments in a mouse model of myocardial I/R injury. A total of 75,546 cells from nine mouse cardiac tissues in 3 different groups are projected by UMAP plot. The four colored tracks (from inside to outside) indicate cluster, origin ID, group and cell type. The axis outside the circular plot depicts the scale of the total cell number for each class. The text in each of the four corners show an overview of the role of SBP on cardiomyocytes, endothelial cells, fibroblasts, and macrophages respectively. Legends denote the origin ID, group, cell type and cluster.

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Section: Discussionmentioning

confidence: 99%

Single-nucleus ribonucleic acid-sequencing and spatial transcriptomics reveal the cardioprotection of Shexiang Baoxin Pill (SBP) in mice with myocardial ischemia-reperfusion injury

et al. 2023

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“…However, studies have shown that myocardial contractility depends on the presence of EE and the degree of its damage regardless of the technique employed to remove the endothelium: immersion in 1% Triton X-100, mechanical peeling, exposure to a high-frequency ultrasound, or flow rate of dry air. Fully removing or partially damaging EE cells directly affect contractile cardiac performance causing a lower contractility of cardiomyocytes [4,5]. The inotropic effect of EE is achieved through the synthesis and release of endothelial mediators, the sensory ability to detect changes in blood plasma and the quality of bloodheart barrier to control transendothelial transport.…”

Section: Introductionmentioning

confidence: 99%

Endocardial endothelium as a blood-heart barrier

et al. 2018

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Introduction. Endocardial endothelium is formed from a single layer of closely related cells with complex interrelationships and extensive overlap at the junctional edges. Morphological characteristics of blood-heart barrier. Endocardium is composed of three layers: endocardial endothelium, subendothelial loose connective tissue and subendocardium. The fibrous component of the subendothelium consists of small amount of collagen and elastic fibers. Several cell types are present in subendocardium: telocytes, fibroblasts and nerve endings. Intercellular bonds between the endocardial endothelial cells. Endocardial endothelial cells are attached to one another via sets of binding proteins forming solid, adherent and communicating connections. Communicating connections form transmembrane channels between the neighboring cells, while solid and adherent connections form pericellular structures like stitches. The maintenance of the presumed transendocardial electrochemical potential difference provides a high gradient for certain ions as well as a selective boundary barrier, basal lamina, preventing ionic leakage. The negatively charged glycocalyx also modulates endothelial permeability. Electrophysiological characteristics of heart-blood barrier. Electrophysiological studies have shown the existence of a large number of membrane ion channels in the endocardial endothelial cells: inward rectifying K + channels, Ca 2+ dependent K + channels, voltage-dependent Clchannels, volume-activated Clchannels, stretch-activated cation channels and one carrier mediated transport mechanism-Na + K + adenosine triphosphatase. Conclusion. Numerous diseases of the cardiovascular system may be a consequence, but also the cause of the endocardial endothelium dysfunction. Selective damage to the endocardial endothelium and subendocardium is found in arrhythmia, atrial fibrillation, ischemia/reperfusion injury and heart failure. Typical lesions of endocardial and microvascular endothelium have also been described in sepsis, myocardial infarction, inflammation and thrombosis. The result of endothelial dysfunction is the weakening of the endothelial barrier regulation and electrolyte imbalance of the subendocardial interstitium.

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Influence of metabolism modifiers of cyclic nucleotides on contractility of right ventricle of rat heart with intact and removed endocardial endothelium

Abstract: An intact endocardial endothelium is necessary for completion of contractile performance of the heart.

Cited by 2 publications

References 10 publications

Single-nucleus ribonucleic acid-sequencing and spatial transcriptomics reveal the cardioprotection of Shexiang Baoxin Pill (SBP) in mice with myocardial ischemia-reperfusion injury

Single-nucleus ribonucleic acid-sequencing and spatial transcriptomics reveal the cardioprotection of Shexiang Baoxin Pill (SBP) in mice with myocardial ischemia-reperfusion injury

Endocardial endothelium as a blood-heart barrier

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