Influence of medium composition on sporulation byStreptomyces coelicolor A3(2) grown on defined solid media

Karandikar, Atul; Sharples, George P.; Hobbs, Glyn

doi:10.1007/bf00765186

Cited by 7 publications

(10 citation statements)

References 10 publications

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Section: Resultssupporting

confidence: 71%

“…On the other hand, substrate mycelia dominated the biomass when strain PCB7 was grown in R2A broth. Indeed, liquid media do not support sporulation of streptomycetes, with only few exceptions (Karandikar et al, 1996). Spores of strains PCB7 harvested from R2A agar plates displayed a higher H 2 uptake rate than the substrate mycelia grown in R2A broth (Fig.…”

Section: Characterization Of Strain Pcb7 H2 Uptake Activitymentioning

confidence: 99%

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Streptomycetes contributing to atmospheric molecular hydrogen soil uptake are widespread and encode a putative high‐affinity [NiFe]‐hydrogenase

Constant

Chowdhury

Pratscher

et al. 2010

Environmental Microbiology

137

194

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Uptake of molecular hydrogen (H2) by soil is a biological reaction responsible for approximately 80% of the global loss of atmospheric H2. Indirect evidence obtained over the last decades suggests that free soil hydrogenases with an unusually high affinity for H2 are carrying out the reaction. This assumption has recently been challenged by the isolation of Streptomyces sp. PCB7, displaying the high-affinity H2 uptake activity previously attributed to free soil enzymes. While this finding suggests that actinobacteria could be responsible for atmospheric H2 soil uptake, the ecological importance of H2-oxidizing streptomycetes remains to be investigated. Here, we show that high-affinity H2 uptake activity is widespread among the streptomycetes. Among 14 streptomycetes strains isolated from temperate forest and agricultural soils, six exhibited a high-affinity H2 uptake activity. The gene encoding the large subunit of a putative high-affinity [NiFe]-hydrogenase (hydB-like gene sequence) was detected exclusively in the isolates exhibiting high-affinity H2 uptake. Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) experiments targeting hydB-like gene transcripts and H2 uptake assays performed with strain PCB7 suggested that streptomycetes spores catalysed the H2 uptake activity. Expression of the activity in term of biomass revealed that 10(6)-10(7) H2-oxidizing bacteria per gram of soil should be sufficient to explain in situ H2 uptake by soil. We propose that specialized H2-oxidizing actinobacteria are responsible for the most important sink term in the atmospheric H2 budget.

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Section: Resultssupporting

confidence: 71%

Section: Characterization Of Strain Pcb7 H2 Uptake Activitymentioning

confidence: 99%

Streptomycetes contributing to atmospheric molecular hydrogen soil uptake are widespread and encode a putative high‐affinity [NiFe]‐hydrogenase

Constant

Chowdhury

Pratscher

et al. 2010

Environmental Microbiology

137

194

View full text Add to dashboard Cite

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“…In addition, removal of the starch from plasterboard (board 5) decreased the sporulation considerably. This finding is supported by the observation that spore formation is restricted under carbon limited conditions (Karandikar et al. 1996).…”

Section: Discussionsupporting

confidence: 63%

“…1998) or sporulation (Kendrick and Ensign 1983). At very low concentrations of phosphate, both the growth and sporulation are reduced (Karandikar et al. 1996).…”

Section: Discussionmentioning

confidence: 99%

Starch in plasterboard sustainsStreptomyces californicusgrowth and bioactivity of spores

Murtoniemi¹,

Keinänen²,

Nevalainen³

et al. 2003

Journal of Applied Microbiology

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Aims: The effects of plasterboard composition on Streptomyces californicus growth and bioactivity of spores were studied. Methods and Results: Streptomyces californicus was grown on 13 modified plasterboards under saturated humidity conditions. The total content of fatty acid methyl esters was used for quantifying S. californicus biomass, while the spore-induced cytotoxicity and production of nitric oxide (NO), tumour necrosis factor-alpha, and interleukine-6 (IL-6) in mouse macrophages was used to assess the bioactivity of spores. Removal of starch completely from the plasterboard or only from the core reduced significantly the biomass production and the biological activity of spores in comparison with reference board. The biocide added into the core or on the liner decreased the growth markedly and inhibited the sporulation totally. The biomass production correlated positively with the spore number, cytotoxicity, and production of NO and IL-6. Conclusions: Streptomyces californicus grew under nutrient limitation on all studied plasterboards. The starch is the major factor enabling S. californicus to grow and to produce biologically active metabolites on plasterboard. Significance and Impact of the Study: The composition of building material has an impact on microbial growth and bioactivity of spores which may be involved in complex mechanisms leading to respiratory symptoms in the occupants in moisture damaged buildings.

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“…It has been demonstrated in pure cultures of Bacillus thuringiensis in a stirred bioreactor that low carbon-to-nitrogen ratios of 4 : 1 resulted in high spore counts [ 19 ]. In contrast however, spore formation in Streptomyces coelicolor was stimulated under nitrogen-limiting conditions [ 20 ]. In particular, C/N ratios between 50 and 100 promoted sporulation, whereas C/N rations <40 did not allow spore formation.…”

Section: Resultsmentioning

confidence: 99%

Detection of Bacterial Endospores in Soil by Terbium Fluorescence

Ammann

Kölle

Brandl

2011

International Journal of Microbiology

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Spore formation is a survival mechanism of microorganisms when facing unfavorable environmental conditions resulting in “dormant” states. We investigated the occurrence of bacterial endospores in soils from various locations including grasslands (pasture, meadow), allotment gardens, and forests, as well as fluvial sediments. Bacterial spores are characterized by their high content of dipicolinic acid (DPA). In the presence of terbium, DPA forms a complex showing a distinctive photoluminescence spectrum. DPA was released from soil by microwaving or autoclaving. The addition of aluminium chloride reduced signal quenching by interfering compounds such as phosphate. The highest spore content (up to 109 spores per gram of dry soil) was found in grassland soils. Spore content is related to soil type, to soil depth, and to soil carbon-to-nitrogen ratio. Our study might provide a basis for the detection of “hot spots” of bacterial spores in soil.

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Influence of medium composition on sporulation byStreptomyces coelicolor A3(2) grown on defined solid media

Cited by 7 publications

References 10 publications

Streptomycetes contributing to atmospheric molecular hydrogen soil uptake are widespread and encode a putative high‐affinity [NiFe]‐hydrogenase

Streptomycetes contributing to atmospheric molecular hydrogen soil uptake are widespread and encode a putative high‐affinity [NiFe]‐hydrogenase

Starch in plasterboard sustainsStreptomyces californicusgrowth and bioactivity of spores

Detection of Bacterial Endospores in Soil by Terbium Fluorescence

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