Influence of Mechanical Scarification and Gibberellic Acid on Seed Germination and Seedling Performance in Pinus gerardiana Wall

Sharma, Lokinder; Reddy, B. Venkateswara; Chatterjee, Moumita; Dhawan, S. C.; Pai, Vinayak

doi:10.20546/ijcmas.2020.904.161

Cited by 3 publications

(2 citation statements)

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“…The lowest seed vigour index (132.81) was recorded for T3 treatment. Similar results were reported by Sharma et al [25,26] on Pinus gerardiana, where mechanical scarification + water soaking treatment shows the highest SVI compared to scarified and non-scarified seeds.…”

Section: Seed Vigour Index (Svi)supporting

confidence: 89%

Effect of Various Pre-sowing Treatments on Germination and Seedling Performance of Bixa orellana L. at Low Seed Moisture Content

Bharti,

Sharma,

Kumar

et al. 2024

JABB

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Natural plant-based substances are becoming more and more popular in today's society for a variety of uses. Bixin (vibrant red-orange dye) is one such compound derived from Bixa orellana L., commonly known as annatto. This plant is primarily propagated through seeds for commercial cultivation. However, there is very little information available on germination combined with the seedling performance of this species. Hence, to fill this research gap, the present study evaluated seed morphological characteristics and various pre-sowing treatments to enhance seed germination and its vigour at a low seed moisture content of 10%. This study used Completely Randomized Design (CRD), and seeds were subjected to six pre-sowing treatments with four replications. The study showed the highest germination (52%) and highest mean daily germination (4.6) in 24hrs Water-soaked seeds, whereas mechanical scarification in combination with 24hrs water-soaking increased the germination percentage from 19% to 47%. In addition, the highest seed vigour index (411.25) was also observed in mechanical scarification combined with 24-hour water soaking. These findings of germination and vigour at low seed moisture content will certainly help in the formation of desiccation and storage protocols for medium to long-term conservation of Bixa orellana L. seeds.

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Section: Seed Vigour Index (Svi)supporting

confidence: 89%

Effect of Various Pre-sowing Treatments on Germination and Seedling Performance of Bixa orellana L. at Low Seed Moisture Content

Bharti,

Sharma,

Kumar

et al. 2024

JABB

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“…GA 3 is commonly used to break seed dormancy and promote seed germination (Leadem, 1987;Sharma et al, 2020;Zhao and Jiang, 2014). Unfortunately, the inclusion of GA 3 at 1 mg • L -1 in the germination medium failed to improve seed germination in the present study (Table 3).…”

Section: Discussioncontrasting

confidence: 55%

Factors Affecting Seed Germination and Establishment of an Efficient Germination Method in Sugar Pine (Pinus lambertiana Dougl.)

Shen¹,

Cho²

2021

horts

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Mature sugar pine (Pinus lambertiana Dougl.) trees produce large amounts of viable seeds but have seed dormancy. In this study, we used three sugar pine genotypes, 8877, 9306, and 9375, to test seed germination response. Seed germination from local sources varied greatly, and germination percentages were poor. There was a large variation in seed size and seed weight among the genotypes. Seeds of 9375 and 9306 were significantly larger and heavier (30.7 and 28.8 g/100 seeds, respectively) than 8877 (23.6 g/100 seeds). Three types of seeds—intact seeds, hulled seeds, and naked embryos—were examined for germination. Intact seeds failed to germinate due to the physical restraint and water impermeability of the seed. Chemical scarification with 5 m hydrochloric acid and 5 m sodium hydroxide did not soften the hard seedcoat and also failed to induce any germination of intact seeds. Hulled seeds resulted in an extremely low germination percentage (≤5%) with abnormal seedling development even though the endosperm was water permeable. Germination of the hulled seeds was not increased by adding 1 mg·L−1 gibberellic acid to the culture medium. Artificial opening of the hulled seeds created by longitudinal or horizontal cuts on the endosperm after removal of the seedcoat to avoid physical restraint and allow air exchange also failed to improve germination, indicating that inhibitors related to germination were present in the endosperm. However, naked embryos of all three genotypes germinated rapidly and uniformly with 70% to 95% germination percentage regardless of cold stratification treatment. Our data indicate that sugar pine seeds from the current source did not have physiological dormancy of embryos themselves, but dormancy was imposed by the seedcoat and endosperm. Using the naked embryos as donor explants, we have successfully established an efficient in vitro culture system. The protocol described here can be applied for the tissue culture and genetic transformation of sugar pine.

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Seed viability and optimal germination conditions of Cedrus atlantica (Manetti ex Endl.) Carrière

et al. 2021

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Our work is part of the ex situ conservation of the Atlas cedar (Cedrus atlantica (Manetti ex Endl.) Carrière), a threatened and endemic Pinaceae of the Maghreb. Our objectives are to evaluate the viability of seeds from three Algerian cedar groves ecotypes (Thienet El Had, Chelia, Chrea) by the 2,3,5 triphenyltetrazolium chloride (TZ) test and to elucidate the effect of continuous temperatures (15°C, 20°C, 25°C) on their germinative capacity. Moreover, the effects of some physicochemical pretreatments in breaking dormancy and improving germinability were estimated on the seeds of the cedar grove of Thienet El Had. In addition, in the same ecotype, the water stress, as simulated by using the PEG6000 (0 to −2.20 bar), was induced upon seed germination. Results of the TZ test showed variability in seeds’ viability among the three ecotypes with rates of 60, 85 and 90% in those of Chelia, Chrea and Thienet El Had, respectively. However, germination tests revealed the existence of partial dormancy in all tested seeds. Dramatic differences in the degree of dormancy were noted among the three provenances investigated. Untreated seeds of the three ecotypes exhibited maximum germination at 20°C: Thienet El Had (52%), Chelia (32%) and Chrea (7%). The final germination percentage was as high as 76% in seeds soaked in hot water (80°C) for 5 min followed by 56% in seeds treated with GA3 (150 ppm). The increase in water stress regularly decreases germination with a tolerance threshold corresponding to −2.2 bar.

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Influence of Mechanical Scarification and Gibberellic Acid on Seed Germination and Seedling Performance in Pinus gerardiana Wall

Cited by 3 publications

References 0 publications

Effect of Various Pre-sowing Treatments on Germination and Seedling Performance of Bixa orellana L. at Low Seed Moisture Content

Effect of Various Pre-sowing Treatments on Germination and Seedling Performance of Bixa orellana L. at Low Seed Moisture Content

Factors Affecting Seed Germination and Establishment of an Efficient Germination Method in Sugar Pine (Pinus lambertiana Dougl.)

Seed viability and optimal germination conditions of Cedrus atlantica (Manetti ex Endl.) Carrière

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