Influence of Matrix Processing on the Optical and Biomechanical Properties of a Corneal Stroma Equivalent

Crabb, Rachael A.B.; Hubel, Allison

doi:10.1089/ten.a.2007.0139

Cited by 24 publications

(12 citation statements)

References 47 publications

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“…In general, the aim of corneal tissue engineering is to fabricate optically, structurally, and biologically features similar to the native tissues. 7–22 However, the previously developed corneas had various difficulties achieving both optical and biochemical properties due to the origins of their applied biomaterials. Therefore, we suggested a Co-dECM hydrogel, not an opaque sponge or a sheet type of material, with a combination of cells to maximize the induction of the cornea recovery after the transplantation.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of cornea-specific bioink: high transparency, improved in vivo safety

et al. 2019

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Corneal transplantation is a typical surgical procedure for severe corneal diseases. However, the waiting time for a donor cornea has gradually increased due to a decrease in supply caused by an aging population and increased cases of laser-based surgeries. Artificial corneas were developed to meet the increase in demand; however, these approaches have suffered from material deterioration resulted by the limited tissue integration. Here, we introduce a cornea-derived decellularized extracellular matrix (Co-dECM) as a bioink for corneal regeneration. The developed Co-dECM bioink had similar quantitative measurement results for collagen and GAGs compared with that of the native cornea and also had the proper transparency for vision. The differentiation potential of human turbinate-derived mesenchymal stem cells (hTMSCs) to a keratocyte lineage was only observed in the Co-dECM group. Moreover, the developed bioink did not have any cytotoxic effect on encapsulated cells for three-dimensional (3D) culture and has great biocompatibility evident by the xeno-implantation of the Co-dECM gel into mice and rabbits for two and one month, respectively. An in vivo safety similar to clinical-grade collagen was seen with the Co-dECM, which helped to maintain the keratocyte-specific characteristics in vivo, compared with collagen. Taken together, the Co-dECM bioink has the potential to be used in various types of corneal diseases based on its corneal-specific ability and design flexibility through 3D cell printing technology.

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Section: Discussionmentioning

confidence: 99%

“…To overcome this limitation, cell-laden collagen scaffolds were suggested. 13–17 Nam et al 13 aligned corneal fibroblasts on a patterned 2-µm thick collagen film fabricated by casting methods. The seeded cells secreted extracellular matrix forming sheet-type structures.…”

Section: Introductionmentioning

confidence: 99%

Characterization of cornea-specific bioink: high transparency, improved in vivo safety

et al. 2019

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“…In spite of the success of several tissue systems, the post-thaw functionality is still difficult to maintain during cryopreservation process (Adham et al, 1996; Crabb and Hubel, 2008; Han and Bischof, 2004; Mikos et al, 2006; Orwin and Hubel, 2000). This challenge is primarily due to the multifaceted aspects of tissue functionality, which include biological and mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Preservation of tissue microstructure and functionality during freezing by modulation of cytoskeletal structure

Park

Seawright

Park

et al. 2015

Journal of the Mechanical Behavior of Biomedical Materials

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Cryopreservation is one of the key enabling technologies for tissue engineering and regenerative medicine, which can provide a reliable long-term storage of engineered tissues (ETs) without losing their functionality. However, it is still extremely difficult to design and develop cryopreservation protocols guaranteeing the post-thaw tissue functionality. One of the major challenges in cryopreservation is associated with the difficulty of identifying effective and less toxic cryoprotective agents (CPAs) to guarantee the post-thaw tissue functionality. In this study, thus, a hypothesis was tested that the modulation of the cytoskeletal structure of cells embedded in the extracellular matrix (ECM) can mitigate the freezing-induced changes of the functionality and can reduce the amount of CPA necessary to preserve the functionality of ETs during cryopreservation. In order to test this hypothesis, we prepared dermal equivalents by seeding fibroblasts in type I collagen matrices resulting in three different cytoskeletal structures. These ETs were exposed to various freeze/thaw (F/T) conditions with and without CPAs. The freezing-induced cell-fluid-matrix interactions and subsequent functional properties of the ETs were assessed. The results showed that the cytoskeletal structure and the use of CPA were strongly correlated to the preservation of the post-thaw functional properties. As the cytoskeletal structure became stronger via stress fiber formation, the ETs functionality was preserved better. It also reduced the necessary CPA concentration to preserve the post-thaw functionality. However, if the extent of the freezing-induced cell-fluid-matrix interaction was too excessive, the cytoskeletal structure was completely destroyed and the beneficial effects became minimal.

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“…Among the scaffolds proposed for corneal regeneration, those based on type I collagen are largely investigated, in an attempt to mimic the composition of the stromal ECM. Some of the earliest trials to produce corneal substitutes involved the use of collagen-based gels ( Germain et al, 1999 ), sponges ( Orwin and Hubel, 2000 ), and films ( Crabb and Hubel, 2008 ), with the collagen derived from animal tissues, such as bovine or porcine skin or tendon. However, the achievement of suitable strength and transparency, prerequisites for corneal function, soon represented major challenges in the development of practical corneal substitutes.…”

Section: Optimization Of Type I Collagen-based Scaffolds For the Development Of Specific Tissue Substitutesmentioning

confidence: 99%

Mimicking the Hierarchical Organization of Natural Collagen: Toward the Development of Ideal Scaffolding Material for Tissue Regeneration

Salvatore

Gallo

Natali

et al. 2021

Front. Bioeng. Biotechnol.

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Biological materials found in living organisms, many of which are proteins, feature a complex hierarchical organization. Type I collagen, a fibrous structural protein ubiquitous in the mammalian body, provides a striking example of such a hierarchical material, with peculiar architectural features ranging from the amino acid sequence at the nanoscale (primary structure) up to the assembly of fibrils (quaternary structure) and fibers, with lengths of the order of microns. Collagen plays a dominant role in maintaining the biological and structural integrity of various tissues and organs, such as bone, skin, tendons, blood vessels, and cartilage. Thus, “artificial” collagen-based fibrous assemblies, endowed with appropriate structural properties, represent ideal substrates for the development of devices for tissue engineering applications. In recent years, with the ultimate goal of developing three-dimensional scaffolds with optimal bioactivity able to promote both regeneration and functional recovery of a damaged tissue, numerous studies focused on the capability to finely modulate the scaffold architecture at the microscale and the nanoscale in order to closely mimic the hierarchical features of the extracellular matrix and, in particular, the natural patterning of collagen. All of these studies clearly show that the accurate characterization of the collagen structure at the submolecular and supramolecular levels is pivotal to the understanding of the relationships between the nanostructural/microstructural properties of the fabricated scaffold and its macroscopic performance. Several studies also demonstrate that the selected processing, including any crosslinking and/or sterilization treatments, can strongly affect the architecture of collagen at various length scales. The aim of this review is to highlight the most recent findings on the development of collagen-based scaffolds with optimized properties for tissue engineering. The optimization of the scaffolds is particularly related to the modulation of the collagen architecture, which, in turn, impacts on the achieved bioactivity.

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Influence of Matrix Processing on the Optical and Biomechanical Properties of a Corneal Stroma Equivalent

Cited by 24 publications

References 47 publications

Characterization of cornea-specific bioink: high transparency, improved in vivo safety

Characterization of cornea-specific bioink: high transparency, improved in vivo safety

Preservation of tissue microstructure and functionality during freezing by modulation of cytoskeletal structure

Mimicking the Hierarchical Organization of Natural Collagen: Toward the Development of Ideal Scaffolding Material for Tissue Regeneration

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