Influence of material properties upon immobilization of histidine-tagged protein on Ni–Co coated chip

Chang, Yaw Jen; Ho, Ching Yuan; Chang, Cheng Hao

doi:10.1016/j.msec.2014.01.028

Cited by 7 publications

(2 citation statements)

References 12 publications

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“…Although diagnostic methods have been established for each of these viral infections, screening cannot be done simultaneously for these and other pathogens [6,23,24]. For diagnostics, protein chips are not yet widely used for animal diseases because of theirs high preparation costs [25,26]. Our study expands the application of protein chips for diagnosing disease in pigs.…”

Section: Discussionmentioning

confidence: 96%

A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

Jing

et al. 2020

BMC Vet Res

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Background At present, pig industry in China is faced with the complex situation of mixed infection caused by multiple pathogens. It is urgent to develop some new high-throughput molecular diagnosis assays to simultaneously detect pathogens or antibodies. Biochip array technology has made it possible to screen thousands of samples simultaneously; it has been twice named as one of the top 10 scientific and technological breakthroughs. Studies have reported encouraging results using protein biochips for detecting antibodies against avian infectious bronchitis virus and ruminant bluetongue virus, but the research of this technology for the diagnosis of swine diseases is still sparse. Results In this study, a novel protein chip was developed that can simultaneously detect the antibodies of four important swine viruses as follow, classical swine fever virus (CSFV), porcine parvovirus (PPV), Japanese encephalitis virus (JEV), and porcine reproductive and respiratory syndrome virus (PRRSV). Four prokaryotic expression plasmids pET-32a-E2 of CSFV, −VP2 of PPV, −EDIII of JEV, and -N of PRRSV were induced by IPTG (Isopropyl β-D-1-Thiogalactopyranoside) and overexpressed in E.coli, respectively. The purified proteins were identified by Western blotting and then printed on epoxy-coated glass slides. The optimized parameters of this diagnostic chip showed that the spotting concentrations of E2、VP2、EDIII、N proteins were 0.2, 0.4, 0.4, and 0.4 mg/mL. The optimal primary and secondary antibody dilutions were 1:50 and 1: 600. Compared with the commercial ELISA (Enzyme-linked immunosorbent assay) kits, the positive and negative coincidence rates of this chip were 95.8% ~ 100 and 86.2% ~ 100%, as well as, no cross-reaction. Conclusion This protein chip provided a fast, specific, and sensitive method for simultaneous detection of antibodies in clinical serum samples. Compared with traditional methods, this protein chip can monitor very small amount of serum.

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Section: Discussionmentioning

confidence: 96%

A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

Jing

et al. 2020

BMC Vet Res

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“…[28][29][30] Different from the reported works, the Ni-Co film developed in our previous works is an alloy type of protein chip with bimetallic elements to enhance the specific binding capability with the histidine (His). [31][32][33] Therefore, the immobilization of different functional proteins with a histidine tag attached becomes feasible. To test the performance of this proposed droplet-based immunoassay chip, two biomaterials were adopted as examples of bioassays: tumor suppressor p53 and kinase ERK1.…”

Section: Introductionmentioning

confidence: 99%

Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins

et al. 2020

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Crystallographic structure of Ni–Co coating on the affinity adsorption of histidine-tagged protein

Chang

Chen

2015

Colloids and Surfaces B: Biointerfaces

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Influence of material properties upon immobilization of histidine-tagged protein on Ni–Co coated chip

Cited by 7 publications

References 12 publications

A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins

Crystallographic structure of Ni–Co coating on the affinity adsorption of histidine-tagged protein

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